##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13189
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.73659868071878	32.0	32.0	32.0	21.0	32.0
2	29.341345060277504	32.0	32.0	32.0	14.0	32.0
3	30.512169231935705	32.0	32.0	32.0	32.0	32.0
4	30.97429676245356	32.0	32.0	32.0	32.0	32.0
5	30.641671089544317	32.0	32.0	32.0	32.0	32.0
6	34.205095155053456	36.0	36.0	36.0	32.0	36.0
7	34.35976950489044	36.0	36.0	36.0	32.0	36.0
8	34.17089999241792	36.0	36.0	36.0	32.0	36.0
9	34.58935476533475	36.0	36.0	36.0	32.0	36.0
10	34.10971263932065	36.0	36.0	36.0	32.0	36.0
11	34.691257866403824	36.0	36.0	36.0	32.0	36.0
12	34.39790734703162	36.0	36.0	36.0	32.0	36.0
13	34.49412389112139	36.0	36.0	36.0	32.0	36.0
14	34.401698385017816	36.0	36.0	36.0	32.0	36.0
15	34.29448783076806	36.0	36.0	36.0	32.0	36.0
16	34.31442869057548	36.0	36.0	36.0	32.0	36.0
17	34.28971112290545	36.0	36.0	36.0	32.0	36.0
18	34.30100841610433	36.0	36.0	36.0	32.0	36.0
19	34.267040715747974	36.0	36.0	36.0	32.0	36.0
20	34.30320721813633	36.0	36.0	36.0	32.0	36.0
21	34.416483433164004	36.0	36.0	36.0	32.0	36.0
22	34.40465539464705	36.0	36.0	36.0	32.0	36.0
23	34.185609219804384	36.0	36.0	36.0	32.0	36.0
24	34.21351125938282	36.0	36.0	36.0	32.0	36.0
25	34.13579498066571	36.0	36.0	36.0	32.0	36.0
26	34.03214800212298	36.0	36.0	36.0	32.0	36.0
27	33.97300780953825	36.0	36.0	36.0	32.0	36.0
28	34.02623398286451	36.0	36.0	36.0	32.0	36.0
29	34.031996360603536	36.0	36.0	36.0	32.0	36.0
30	33.8601106983092	36.0	36.0	36.0	32.0	36.0
31	33.87618469937069	36.0	36.0	36.0	32.0	36.0
32	33.870649783910835	36.0	36.0	36.0	32.0	36.0
33	33.86754113276215	36.0	36.0	36.0	32.0	36.0
34	33.85260444309652	36.0	36.0	36.0	32.0	36.0
35	33.828114337705664	36.0	36.0	36.0	32.0	36.0
36	33.74387747365228	36.0	36.0	36.0	32.0	36.0
37	33.70331336719994	36.0	36.0	36.0	32.0	36.0
38	33.64007885359011	36.0	36.0	36.0	27.0	36.0
39	33.7676093714459	36.0	36.0	36.0	32.0	36.0
40	33.73060884070058	36.0	36.0	36.0	32.0	36.0
41	33.69444233831223	36.0	36.0	36.0	27.0	36.0
42	33.55030707407688	36.0	36.0	36.0	27.0	36.0
43	33.4553036621427	36.0	36.0	36.0	21.0	36.0
44	33.40746076275684	36.0	36.0	36.0	21.0	36.0
45	33.2914550003791	36.0	36.0	36.0	21.0	36.0
46	33.34233073015392	36.0	36.0	36.0	21.0	36.0
47	33.22192736371218	36.0	36.0	36.0	21.0	36.0
48	33.25202820532262	36.0	36.0	36.0	21.0	36.0
49	33.09318371370081	36.0	36.0	36.0	14.0	36.0
50	32.2434604594738	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	3.0
23	11.0
24	19.0
25	41.0
26	87.0
27	129.0
28	255.0
29	409.0
30	641.0
31	976.0
32	1366.0
33	2017.0
34	3173.0
35	4061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79998483584805	20.00909849116688	12.358783835014027	25.832132837971038
2	19.061338994616726	21.09333535522026	36.03760709682311	23.807718553339903
3	21.57858821745394	23.929031768898323	28.827052847069528	25.665327166578212
4	14.32254151186595	17.908863446811736	36.86405337781485	30.904541663507466
5	18.34862385321101	34.1724164076124	31.450451133520357	16.028508605656228
6	33.92220790052317	37.28864963226932	15.611494427174161	13.17764804003336
7	28.796724543179923	31.02585487906589	20.137993782697702	20.039426795056485
8	28.19015846538782	32.3375540222913	18.59125028432785	20.881037227993023
9	26.582758359238763	13.769049965880658	16.99901433012359	42.64917734475699
10	14.716809462430813	25.27864129198575	32.98203047994541	27.022518765638033
11	37.23557510046251	20.60808249298658	22.116915611494427	20.039426795056485
12	24.376374251269997	23.41345060277504	28.129501857608613	24.08067328834635
13	29.350216089165215	18.826294639472287	25.566760178936992	26.256729092425505
14	23.466525134581847	18.871787095306694	25.961028129501855	31.700659640609597
15	25.6198347107438	26.2264007885359	22.26097505497005	25.892789445750246
16	24.664493138221243	26.62066873910077	23.921449692925922	24.793388429752067
17	22.715899613314125	25.665327166578212	25.27105921601334	26.347714004094318
18	24.535597846690425	25.19523845628933	25.6274167867162	24.64174691030404
19	25.142163924482524	25.369626203654562	25.938281901584652	23.54992797027826
20	25.528849799074987	24.232314807794374	24.998104481006898	25.240730912123738
21	26.62825081507317	24.92986579725529	23.70915156569869	24.732731821972855
22	25.149746000454925	25.25589506406854	25.521267723102586	24.07309121237395
23	23.89870346500872	24.368792175297596	25.642580938661002	26.08992342103268
24	23.731897793615893	25.877625293805444	25.0208507089241	25.369626203654562
25	23.693987413753888	25.695655470467816	24.907119569338086	25.703237546440217
26	24.255061035711577	25.399954507544166	25.0208507089241	25.324133747820156
27	24.899537493365685	25.301387519902953	24.891955417393284	24.907119569338086
28	24.16407612404276	24.520433694745623	26.597922511183565	24.717567670028053
29	24.846462961558874	24.75547804989006	26.54484797937675	23.853211009174313
30	24.64174691030404	25.377208279626963	25.763894154219425	24.21715065584957
31	24.02001668056714	25.695655470467816	25.6274167867162	24.65691106224884
32	24.619000682386837	25.536431875047388	24.596254454469634	25.24831298809614
33	23.519599666388658	25.301387519902953	25.25589506406854	25.92311774963985
34	24.80855258169687	25.142163924482524	25.91553567366745	24.133747820153157
35	25.945863977557053	24.76306012586246	24.899537493365685	24.3915384032148
36	23.800136477367502	25.99135643339146	24.747895973917657	25.460611115323378
37	25.832132837971038	25.6274167867162	24.3991204791872	24.14132989612556
38	23.99909008189263	24.772520473157417	26.2208067940552	25.007582650894754
39	25.35446205170976	25.225566760178936	24.32329971946319	25.09667146864812
40	23.95177799681553	26.650997042990372	25.225566760178936	24.171658200015163
41	25.11941769656532	24.92986579725529	25.900371521722647	24.050344984456746
42	25.301387519902953	25.892789445750246	24.891955417393284	23.91386761695352
43	24.672075214193644	25.08908939267571	25.453029039350973	24.785806353779666
44	24.15649404807036	25.763894154219425	24.747895973917657	25.331715823792557
45	24.854045037531275	26.499355523542345	24.64174691030404	24.004852528622337
46	24.982940329062096	25.92311774963985	24.838880885586473	24.255061035711577
47	24.33088179543559	26.08234134506028	24.990522405034497	24.596254454469634
48	24.891955417393284	26.45386306770794	24.626582758359238	24.02759875653954
49	24.952612025172492	25.961028129501855	24.975358253089695	24.111001592235954
50	24.414284631132006	25.09667146864812	25.46819319129578	25.0208507089241
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	1.5
4	0.0
5	1.5
6	3.0
7	3.5
8	4.0
9	4.5
10	5.0
11	8.0
12	11.0
13	13.0
14	15.0
15	22.5
16	30.0
17	33.5
18	37.0
19	45.0
20	53.0
21	58.5
22	64.0
23	77.0
24	90.0
25	106.5
26	123.0
27	152.0
28	181.0
29	196.0
30	211.0
31	218.5
32	226.0
33	258.5
34	291.0
35	312.5
36	334.0
37	397.5
38	461.0
39	532.0
40	603.0
41	654.0
42	705.0
43	708.0
44	711.0
45	785.5
46	860.0
47	910.5
48	961.0
49	962.0
50	963.0
51	951.0
52	939.0
53	906.5
54	874.0
55	901.0
56	928.0
57	895.0
58	862.0
59	780.5
60	699.0
61	621.0
62	543.0
63	458.0
64	373.0
65	330.0
66	287.0
67	240.0
68	193.0
69	186.0
70	179.0
71	136.0
72	93.0
73	83.0
74	73.0
75	67.0
76	61.0
77	53.5
78	46.0
79	39.5
80	33.0
81	29.0
82	25.0
83	18.5
84	12.0
85	11.5
86	11.0
87	5.5
88	0.0
89	2.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	3.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0075820759724012435
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	13189.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.12813708393358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.72533019264743	85.374175449238
2	3.4239084714393875	6.171809841534612
3	0.7655421889459073	2.0699067404655396
4	0.2944393034407336	1.0614906361361742
5	0.2944393034407336	1.3268632951702175
6	0.15142592748380584	0.8188642050193343
7	0.13460082443004964	0.8491925089089393
8	0.04206275763439051	0.30328303889604974
9	0.04206275763439051	0.341193418758056
>10	0.12618827290317153	1.6832208658730763
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	30	0.2274622791720373	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	24	0.18196982333762984	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	20	0.15164151944802487	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	20	0.15164151944802487	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	15	0.11373113958601865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0075820759724012435	0.0
31	0.0	0.0	0.0	0.0075820759724012435	0.0
32	0.0	0.0	0.0	0.03791037986200622	0.0
33	0.0	0.0	0.0	0.06065660777920995	0.0
34	0.0	0.0	0.0	0.07582075972401243	0.0
35	0.0	0.0	0.0	0.07582075972401243	0.0
36	0.0	0.0	0.0	0.10614906361361741	0.0
37	0.0	0.0	0.0	0.1592235954204261	0.0
38	0.0	0.0	0.0	0.19713397528243232	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAG	30	0.0055434094	29.333336	6
>>END_MODULE