FastQCFastQC Report
Thu 2 Feb 2017
SRR4064198_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064198_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293987
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44030.3402661695983035No Hit
TATCAACGCAGAGTACTTTTTTTTT32360.25007979214628895No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29680.22936861034925388No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18910.14613748051564662No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16560.12797655617869422No Hit
GTACATGGGGTGGTATCAACGCAAA16360.12643094559682594No Hit
GGTATCAACGCAGAGTACTTTTTTT16130.12465349342767741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG601.467497E-615.8321425
AGAACCG851.7173079E-814.52831655
CAACGGA551.9577888E-413.81981414
AACCGCG707.2601324E-613.5714567
TATACAG1205.0931703E-1113.457325
GCGTTAT1001.0119038E-813.2948851
TTATACG500.001505854813.2923152
AGTGACG1207.385097E-1012.668653519
GAACCGC1001.4423676E-712.3495476
TCTAGCG701.08903165E-412.21620117
CTAGCGG701.08903165E-412.21620118
GTCTTAA701.09455075E-412.2095881
TCGTTAA1103.804962E-812.09233712
CCGGTTT550.00306525412.09233712
AATCGAA951.039285E-612.0000257
GTATTAG1850.011.8062681
AGGACCT5400.011.7861495
CGGTCCA2500.011.78139110
TCCAACG1052.712768E-711.76374918
TGCGCCC658.0138753E-411.69368810
Produced by FastQC (version 0.11.5)