##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064198_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1293987
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193405343330344	32.0	32.0	32.0	32.0	32.0
2	30.73117272430094	32.0	32.0	32.0	32.0	32.0
3	30.75484993280458	32.0	32.0	32.0	32.0	32.0
4	30.791620781352517	32.0	32.0	32.0	32.0	32.0
5	30.677322106018067	32.0	32.0	32.0	32.0	32.0
6	34.33877001855505	36.0	36.0	36.0	32.0	36.0
7	34.261077584241576	36.0	36.0	36.0	32.0	36.0
8	34.212235517049244	36.0	36.0	36.0	32.0	36.0
9	34.36971623362522	36.0	36.0	36.0	32.0	36.0
10	34.02006047974207	36.0	36.0	36.0	32.0	36.0
11	34.37126029859651	36.0	36.0	36.0	32.0	36.0
12	34.14749993624356	36.0	36.0	36.0	32.0	36.0
13	34.25430085464537	36.0	36.0	36.0	32.0	36.0
14	34.14209339042819	36.0	36.0	36.0	32.0	36.0
15	34.070415699694045	36.0	36.0	36.0	32.0	36.0
16	34.07576505791789	36.0	36.0	36.0	32.0	36.0
17	33.988470517864556	36.0	36.0	36.0	32.0	36.0
18	33.989200046059196	36.0	36.0	36.0	32.0	36.0
19	34.00338334156371	36.0	36.0	36.0	32.0	36.0
20	33.98709724286256	36.0	36.0	36.0	32.0	36.0
21	33.95731564536583	36.0	36.0	36.0	32.0	36.0
22	33.9362296530027	36.0	36.0	36.0	32.0	36.0
23	33.889677407887405	36.0	36.0	36.0	32.0	36.0
24	33.870170256733644	36.0	36.0	36.0	32.0	36.0
25	33.476267535918055	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	9.0
5	12.0
6	56.0
7	21.0
8	66.0
9	72.0
10	102.0
11	43.0
12	81.0
13	68.0
14	140.0
15	236.0
16	373.0
17	491.0
18	684.0
19	913.0
20	1418.0
21	2150.0
22	3326.0
23	5215.0
24	7585.0
25	11174.0
26	15757.0
27	20591.0
28	28321.0
29	37941.0
30	49846.0
31	67230.0
32	95414.0
33	133816.0
34	275985.0
35	534845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.952577982701094	17.831729921793098	11.695704341426156	25.519987754079654
2	15.79394398417213	20.358909360702363	38.926054160999136	24.921092494126377
3	18.366542577979192	25.18804575387162	29.200744686688303	27.244666981460885
4	12.142333825884274	16.11870374606629	37.29543164155481	34.44353078649463
5	13.700582437830017	37.64452743393473	34.32371981154241	14.33117031669284
6	32.27287693594288	36.87948963297661	17.790424280460716	13.057209150619794
7	28.897518564581244	31.25296140530421	21.767034312494058	18.082485717620486
8	27.22148738480425	33.77147853325592	20.19413107558295	18.81290300635688
9	27.614502134713486	14.155524044713783	19.161547013952568	39.06842680662017
10	15.43142529515968	27.173170914641265	32.71418552629239	24.68121826390666
11	35.92686953193562	21.39155110356799	24.025423237364734	18.656156127131652
12	24.613371668160415	24.10338339905055	30.014607548754334	21.2686373840347
13	29.31904419821574	20.269224255855836	25.89288406948052	24.518847476447906
14	22.89842161714066	20.52910740287931	26.354145159257293	30.21832582072274
15	24.254351100649842	28.63305692077858	23.12331237299302	23.989279605578563
16	24.26477468246757	26.228159375181242	25.59181858603824	23.915247356312953
17	22.90873098723332	26.637127767338637	26.439016091740708	24.015125153687336
18	23.500878560645432	25.594575197366794	28.016572004850644	22.88797423713713
19	24.959398384597062	25.211051063684987	26.35562993599639	23.473920615721564
20	25.191434796159935	25.171326505279584	26.151218987795037	23.486019710765436
21	26.050117319878797	24.674296196732367	25.20784256708578	24.067743916303055
22	25.159453153216553	25.44986013288343	25.774682853149965	23.616003860750045
23	23.589065358036436	25.337076696404747	26.491950609263277	24.58190733629554
24	24.1796239080922	25.673937673767533	26.451024140877855	23.69541427726241
25	24.000931146942566	25.444904947994207	26.59414444687629	23.96001945818694
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	342.0
1	342.0
2	636.5
3	931.0
4	931.0
5	931.0
6	2452.5
7	3974.0
8	3974.0
9	3974.0
10	3897.5
11	3821.0
12	3821.0
13	3821.0
14	4045.5
15	4270.0
16	4270.0
17	4270.0
18	7665.5
19	11061.0
20	11061.0
21	11061.0
22	17550.0
23	24039.0
24	24039.0
25	24039.0
26	36518.0
27	48997.0
28	48997.0
29	48997.0
30	61395.5
31	73794.0
32	73794.0
33	73794.0
34	85420.0
35	97046.0
36	97046.0
37	97046.0
38	106752.5
39	116459.0
40	116459.0
41	116459.0
42	129198.5
43	141938.0
44	141938.0
45	141938.0
46	156065.5
47	170193.0
48	170193.0
49	170193.0
50	169167.0
51	168141.0
52	168141.0
53	168141.0
54	151590.0
55	135039.0
56	135039.0
57	135039.0
58	121760.0
59	108481.0
60	108481.0
61	108481.0
62	94480.0
63	80479.0
64	80479.0
65	80479.0
66	65883.0
67	51287.0
68	51287.0
69	51287.0
70	38471.0
71	25655.0
72	25655.0
73	25655.0
74	19638.0
75	13621.0
76	13621.0
77	13621.0
78	10829.0
79	8037.0
80	8037.0
81	8037.0
82	5500.5
83	2964.0
84	2964.0
85	2964.0
86	2304.0
87	1644.0
88	1644.0
89	1644.0
90	1178.5
91	713.0
92	713.0
93	713.0
94	506.0
95	299.0
96	299.0
97	299.0
98	530.5
99	762.0
100	762.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038253861901240124
2	0.005177795449258764
3	0.0015456105818682877
4	0.003323062751016819
5	0.008887260845742654
6	0.013369531533160688
7	0.02009293756428774
8	0.033076066451981356
9	0.0467547201015157
10	0.054405492481763726
11	0.052705320841708606
12	0.0633700338565998
13	0.061051617983797365
14	0.06398827808934711
15	0.0580376773491542
16	0.06275178962385247
17	0.06004697110558297
18	0.07442114951695805
19	0.07225729470234245
20	0.0763531627442934
21	0.07272097787690293
22	0.07527123533698561
23	0.07975350602440365
24	0.07357106369693049
25	0.07403474687149098
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1293987.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.52272725304752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.50676724468944	40.948508362499034
2	14.091312009320106	15.08410898624866
3	4.390883419478011	7.050361669819506
4	1.7850439524422526	3.821616824050744
5	0.8574736959238329	2.294716537679695
6	0.5481874045292857	1.7604290961706193
7	0.33601541824727565	1.2589123108567337
8	0.22686761265039798	0.9714058683549828
9	0.1783131632586358	0.8589426122448088
>10	0.8967867808431588	8.832311830342553
>50	0.09028166435613712	3.4127641137404936
>100	0.0820791766789678	8.863831913826472
>500	0.00796181401504203	2.906803517226617
>1k	0.002026643567465244	1.9352863569391594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4403	0.3402661695983035	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3236	0.25007979214628895	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2968	0.22936861034925388	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1891	0.14613748051564662	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1656	0.12797655617869422	No Hit
GTACATGGGGTGGTATCAACGCAAA	1636	0.12643094559682594	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1613	0.12465349342767741	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.728052909341439E-5
2	0.0	0.0	0.0	0.0	7.728052909341439E-5
3	0.0	0.0	0.0	0.0	7.728052909341439E-5
4	0.0	0.0	0.0	0.0	7.728052909341439E-5
5	0.0	0.0	0.0	0.0	7.728052909341439E-5
6	0.0	0.0	0.0	0.0	7.728052909341439E-5
7	0.0	0.0	0.0	0.0	7.728052909341439E-5
8	0.0	0.0	0.0	0.0	7.728052909341439E-5
9	0.0	0.0	0.0	0.0	7.728052909341439E-5
10	0.0	0.0	0.0	0.0	7.728052909341439E-5
11	0.0	0.0	0.0	0.0	7.728052909341439E-5
12	0.0	0.0	0.0	0.0	2.3184158728024315E-4
13	0.0	0.0	0.0	0.0	3.0912211637365756E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCACCG	60	1.467497E-6	15.832142	5
AGAACCG	85	1.7173079E-8	14.5283165	5
CAACGGA	55	1.9577888E-4	13.819814	14
AACCGCG	70	7.2601324E-6	13.571456	7
TATACAG	120	5.0931703E-11	13.45732	5
GCGTTAT	100	1.0119038E-8	13.294885	1
TTATACG	50	0.0015058548	13.292315	2
AGTGACG	120	7.385097E-10	12.6686535	19
GAACCGC	100	1.4423676E-7	12.349547	6
TCTAGCG	70	1.08903165E-4	12.216201	17
CTAGCGG	70	1.08903165E-4	12.216201	18
GTCTTAA	70	1.09455075E-4	12.209588	1
TCGTTAA	110	3.804962E-8	12.092337	12
CCGGTTT	55	0.003065254	12.092337	12
AATCGAA	95	1.039285E-6	12.000025	7
GTATTAG	185	0.0	11.806268	1
AGGACCT	540	0.0	11.786149	5
CGGTCCA	250	0.0	11.781391	10
TCCAACG	105	2.712768E-7	11.763749	18
TGCGCCC	65	8.0138753E-4	11.693688	10
>>END_MODULE