FastQCFastQC Report
Thu 2 Feb 2017
SRR4064198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293987
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13510.10440599480520284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC3350.020.358281
CTAAGAC2350.018.7220233
CGCAAAT1658.185452E-1118.66817523
GACGTGA4300.018.4172467
TAGGACG8250.018.1319944
TTAGGAC5350.017.6809393
TAACGGC1751.9099389E-1017.60074236
TTAACGG1751.9099389E-1017.60074235
ACTATAC1509.606083E-917.5987013
ACCGTCG2650.017.4326768
ATACACT5050.017.4244564
ATTGTCG1408.37681E-817.28510743
AGGACGT8500.017.081095
GATATAT1551.4391844E-817.0323181
GGACGTG8450.016.921836
TAAGACC1954.9112714E-1116.9218274
GTCCTAC8750.016.8457741
GTATCAA37250.016.7732121
GTGTATA5550.016.6487061
TCCTAAA5650.016.3527762
Produced by FastQC (version 0.11.5)