##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064198_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1293987
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262657198256242	32.0	32.0	32.0	32.0	32.0
2	31.403572833421048	32.0	32.0	32.0	32.0	32.0
3	31.474671692992278	32.0	32.0	32.0	32.0	32.0
4	31.562509515165143	32.0	32.0	32.0	32.0	32.0
5	31.507409270726832	32.0	32.0	32.0	32.0	32.0
6	35.088633038817235	36.0	36.0	36.0	36.0	36.0
7	35.11186202025213	36.0	36.0	36.0	36.0	36.0
8	35.055502103189596	36.0	36.0	36.0	36.0	36.0
9	35.17120496573768	36.0	36.0	36.0	36.0	36.0
10	35.02308214842962	36.0	36.0	36.0	36.0	36.0
11	35.16519022215834	36.0	36.0	36.0	36.0	36.0
12	35.07422253855719	36.0	36.0	36.0	36.0	36.0
13	35.12016040346619	36.0	36.0	36.0	36.0	36.0
14	35.065936520227794	36.0	36.0	36.0	36.0	36.0
15	35.04181340307128	36.0	36.0	36.0	36.0	36.0
16	35.05392326198022	36.0	36.0	36.0	36.0	36.0
17	35.01463229537855	36.0	36.0	36.0	36.0	36.0
18	35.00873501820343	36.0	36.0	36.0	36.0	36.0
19	35.00362213839861	36.0	36.0	36.0	36.0	36.0
20	34.995411082182436	36.0	36.0	36.0	36.0	36.0
21	34.98678580233032	36.0	36.0	36.0	36.0	36.0
22	34.97468753550074	36.0	36.0	36.0	36.0	36.0
23	34.926440528382436	36.0	36.0	36.0	36.0	36.0
24	34.895687514635	36.0	36.0	36.0	32.0	36.0
25	34.87263241439056	36.0	36.0	36.0	32.0	36.0
26	34.8176558187988	36.0	36.0	36.0	32.0	36.0
27	34.80397716514926	36.0	36.0	36.0	32.0	36.0
28	34.78040196694403	36.0	36.0	36.0	32.0	36.0
29	34.74577333466256	36.0	36.0	36.0	32.0	36.0
30	34.718321745118	36.0	36.0	36.0	32.0	36.0
31	34.71304580339679	36.0	36.0	36.0	32.0	36.0
32	34.664230784389645	36.0	36.0	36.0	32.0	36.0
33	34.64256364244772	36.0	36.0	36.0	32.0	36.0
34	34.62042895330479	36.0	36.0	36.0	32.0	36.0
35	34.58076935857934	36.0	36.0	36.0	32.0	36.0
36	34.54313683213201	36.0	36.0	36.0	32.0	36.0
37	34.51821386149938	36.0	36.0	36.0	32.0	36.0
38	34.47622039479531	36.0	36.0	36.0	32.0	36.0
39	34.457319122989645	36.0	36.0	36.0	32.0	36.0
40	34.43674550053439	36.0	36.0	36.0	32.0	36.0
41	34.40309833097241	36.0	36.0	36.0	32.0	36.0
42	34.33802039742285	36.0	36.0	36.0	32.0	36.0
43	34.331450006839326	36.0	36.0	36.0	32.0	36.0
44	34.25183869698846	36.0	36.0	36.0	32.0	36.0
45	34.205607938874195	36.0	36.0	36.0	32.0	36.0
46	34.161903481256	36.0	36.0	36.0	32.0	36.0
47	34.11855219565575	36.0	36.0	36.0	32.0	36.0
48	34.05939085941358	36.0	36.0	36.0	32.0	36.0
49	34.035971767877115	36.0	36.0	36.0	32.0	36.0
50	33.450973618745785	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	12.0
21	32.0
22	135.0
23	395.0
24	1139.0
25	2571.0
26	5306.0
27	9642.0
28	16052.0
29	24134.0
30	34229.0
31	47529.0
32	68335.0
33	108642.0
34	222588.0
35	753243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02550702190854	18.676398643149785	12.243107740368304	26.05498659457337
2	15.121052501421964	21.145624675421026	38.36353463412222	25.369788189034796
3	17.6033632668202	25.814773025858205	29.509575109352543	27.072288597969056
4	11.739839735638766	16.870262220563266	37.41080860936006	33.9790894344379
5	13.266284746291888	38.13600909437266	34.01772969898461	14.579976460350839
6	32.391770216455704	37.15017894361827	17.464448914707535	12.993601925218492
7	28.79155663851337	31.40819807308729	21.845119000422724	17.95512628797662
8	27.176934544164666	34.04616893369099	19.94100404409009	18.835892478054262
9	27.564970493951957	14.019932425122029	19.0867344888452	39.328362592080815
10	15.345350567051142	27.359802160010823	32.76547073938832	24.529376533549723
11	36.0900349771945	21.358809137038577	23.946472372962305	18.60468351280462
12	24.572290581196395	24.193461134819078	30.021035776275784	21.21321250770874
13	29.466575165883807	20.251117097817737	25.880038145934474	24.40226959036398
14	22.859966908477443	20.589619524771113	26.38890498899912	30.16150857775233
15	24.334997754924288	28.651803054694064	23.074741313952778	23.93845787642887
16	24.361308650714776	26.239321655612862	25.542398842635166	23.856970851037193
17	22.885392202549177	26.65111782421307	26.41981720063648	24.043672772601273
18	23.538603615961545	25.547436794089577	28.075750491698127	22.838209098250754
19	24.99153777552462	25.161786912686846	26.473083943721182	23.373591368067352
20	25.208754674520456	24.993218612609287	26.375230586491476	23.422796126378785
21	26.014094422896054	24.530153703244313	25.494228303684658	23.96152357017497
22	25.073126926706145	25.35605181830055	25.96950655456834	23.601314700424965
23	23.512596378777772	25.340672945471464	26.562791010391944	24.583939665358816
24	24.23014830952184	25.569501299476116	26.583888660051947	23.616461730950096
25	24.080215089828133	25.30798603678135	26.731124576980474	23.880674296410042
26	23.4256597958302	26.17396862100759	27.122015761370655	23.27835582179156
27	23.96695620050405	25.949383230325857	26.265477205296467	23.818183363873626
28	23.365006742997135	25.75701252381302	26.748252423807994	24.129728309381846
29	23.73194019930987	25.41382494889738	26.411147133036827	24.44308771875592
30	23.432603395043838	25.76338252398672	26.869843772339628	23.93417030862981
31	24.403855636224804	25.349447947210678	25.942539520116732	24.304156896447786
32	23.662424861624828	26.02867507608509	25.99358860416123	24.315311458128853
33	23.32604026669284	25.73066558056391	26.76140697707669	24.181887175666557
34	24.14015623406051	25.576449276482354	26.8610370058951	23.42235748356204
35	24.435197986937663	25.66863145301708	26.53814022036417	23.358030339681086
36	23.513863599125738	26.122350242524107	26.339756332058627	24.024029826291528
37	24.410077430114665	25.844532324116788	25.758902453821307	23.986487791947237
38	23.801011220497696	26.194815292275493	26.146742902366675	23.857430584860133
39	24.452696619227396	25.595798861612057	25.736455076955178	24.215049442205366
40	24.84651671777624	25.764015975863092	26.251282891482834	23.138184414877834
41	23.865425293077053	25.798045199827197	26.811768370013205	23.524761137082546
42	24.52974252920465	26.621441576788367	26.02714741895497	22.82166847505201
43	23.53680806881772	25.91298555065848	26.432472493545056	24.117733886978744
44	23.40069707953044	26.270006259805097	25.94766493813612	24.381631722528343
45	23.687143153020312	26.497636734025154	25.907670335988726	23.90754977696581
46	23.695707110713716	25.832375691376274	25.869547913139108	24.6023692847709
47	24.209310143628304	25.851472025904876	26.065079040004946	23.874138790461874
48	24.244321154525338	26.38873110561381	25.248286473632675	24.11866126622817
49	23.889262841938418	26.313483252871173	25.571046914835822	24.226206990354587
50	23.649812050993532	26.981733705933998	25.238645777948143	24.12980846512433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	146.0
1	93.0
2	40.0
3	174.0
4	308.0
5	348.0
6	388.0
7	384.0
8	380.0
9	505.0
10	630.0
11	975.5
12	1321.0
13	2205.5
14	3090.0
15	4059.0
16	5028.0
17	5411.5
18	5795.0
19	5925.5
20	6056.0
21	6610.0
22	7164.0
23	7992.0
24	8820.0
25	9902.0
26	10984.0
27	13884.5
28	16785.0
29	20193.5
30	23602.0
31	26675.5
32	29749.0
33	35104.5
34	40460.0
35	46696.5
36	52933.0
37	57700.0
38	62467.0
39	64383.5
40	66300.0
41	69513.0
42	72726.0
43	72002.5
44	71279.0
45	76754.5
46	82230.0
47	86093.0
48	89956.0
49	91657.0
50	93358.0
51	89984.0
52	86610.0
53	84873.0
54	83136.0
55	81864.0
56	80592.0
57	78265.5
58	75939.0
59	68634.5
60	61330.0
61	53734.5
62	46139.0
63	39768.5
64	33398.0
65	28334.5
66	23271.0
67	20021.5
68	16772.0
69	15022.5
70	13273.0
71	10440.5
72	7608.0
73	6515.5
74	5423.0
75	4186.5
76	2950.0
77	2598.5
78	2247.0
79	1823.5
80	1400.0
81	1126.5
82	853.0
83	693.0
84	533.0
85	397.0
86	261.0
87	184.0
88	107.0
89	82.5
90	58.0
91	45.5
92	33.0
93	25.0
94	17.0
95	19.5
96	22.0
97	16.0
98	10.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008346297142088753
2	2.3184158728024317E-4
3	5.409637036539007E-4
4	0.0
5	0.0
6	3.0912211637365756E-4
7	0.0
8	0.0
9	0.0011592079364012159
10	9.273663491209727E-4
11	7.728052909341439E-5
12	7.728052909341439E-5
13	6.955247618407295E-4
14	0.0
15	0.003400343280110233
16	0.001004646878214387
17	0.0
18	1.5456105818682878E-4
19	7.728052909341439E-5
20	3.0912211637365756E-4
21	0.0
22	3.0912211637365756E-4
23	3.0912211637365756E-4
24	3.0912211637365756E-4
25	0.0012364884654946303
26	0.004945953861978521
27	0.005255075978352178
28	0.004018587512857548
29	0.0017001716400551164
30	0.003245782221923404
31	0.00687796708931388
32	0.00316850169282999
33	0.004945953861978521
34	0.002395696401895846
35	0.0029366601055497467
36	0.007341650263874368
37	0.0035549043382970616
38	0.008114455554808511
39	0.004018587512857548
40	0.0027048185182695036
41	0.003400343280110233
42	0.0011592079364012159
43	0.001391049523681459
44	0.0013137689945880444
45	0.0011592079364012159
46	7.728052909341439E-4
47	0.0017001716400551164
48	0.001391049523681459
49	3.0912211637365756E-4
50	0.0014683300527748733
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1293987.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.95260612094667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.59893536900766	44.970605368448986
2	13.732767447889149	15.916993257161558
3	4.095183384200811	7.119796489729051
4	1.6942337844365218	3.927410527450025
5	0.8360040080948471	2.422430549832669
6	0.484370106793423	1.6842306009456072
7	0.29352820165179533	1.1907506979001412
8	0.20766997601410053	0.9628013058473288
9	0.15077902518106528	0.7864233711856717
>10	0.765215842879828	8.06557411020139
>50	0.06890126083121788	2.7934171928440374
>100	0.0665114060364685	7.65429870547646
>500	0.005229711189513356	2.0684506993045186
>1k	6.704757935273533E-4	0.4368171236724912
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1351	0.10440599480520284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.3184158728024315E-4
13	0.0	0.0	0.0	0.0	3.0912211637365756E-4
14	0.0	0.0	0.0	0.0	3.0912211637365756E-4
15	0.0	0.0	0.0	7.728052909341439E-5	3.864026454670719E-4
16	0.0	0.0	0.0	7.728052909341439E-5	3.864026454670719E-4
17	0.0	0.0	0.0	1.5456105818682878E-4	3.864026454670719E-4
18	0.0	0.0	0.0	3.864026454670719E-4	3.864026454670719E-4
19	0.0	0.0	0.0	3.864026454670719E-4	3.864026454670719E-4
20	0.0	0.0	0.0	5.409637036539007E-4	4.636831745604863E-4
21	0.0	0.0	0.0	6.182442327473151E-4	4.636831745604863E-4
22	0.0	0.0	0.0	8.500858200275582E-4	4.636831745604863E-4
23	0.0	0.0	0.0	0.0023184158728024313	4.636831745604863E-4
24	0.0	0.0	0.0	0.00440499015832462	4.636831745604863E-4
25	0.0	0.0	0.0	0.006027881269286322	4.636831745604863E-4
26	0.0	0.0	0.0	0.007418930792967781	6.182442327473151E-4
27	0.0	0.0	0.0	0.009119102433022898	6.182442327473151E-4
28	0.0	0.0	0.0	0.013524092591347518	6.182442327473151E-4
29	0.0	0.0	0.0	0.021252145500688956	6.182442327473151E-4
30	0.0	0.0	0.0	0.0343898354465694	6.182442327473151E-4
31	0.0	0.0	0.0	0.06321547279841297	6.955247618407295E-4
32	0.0	0.0	0.0	0.09590513660492725	6.955247618407295E-4
33	0.0	0.0	0.0	0.12519445713133132	6.955247618407295E-4
34	0.0	0.0	0.0	0.15425193607045512	9.273663491209726E-4
35	0.0	0.0	0.0	0.1901873820988928	9.273663491209726E-4
36	0.0	0.0	0.0	0.24096068971326606	9.273663491209726E-4
37	0.0	0.0	0.0	0.32048235415038945	9.273663491209726E-4
38	0.0	0.0	0.0	0.4275158869447684	9.273663491209726E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	335	0.0	20.35828	1
CTAAGAC	235	0.0	18.722023	3
CGCAAAT	165	8.185452E-11	18.668175	23
GACGTGA	430	0.0	18.417246	7
TAGGACG	825	0.0	18.131994	4
TTAGGAC	535	0.0	17.680939	3
TAACGGC	175	1.9099389E-10	17.600742	36
TTAACGG	175	1.9099389E-10	17.600742	35
ACTATAC	150	9.606083E-9	17.598701	3
ACCGTCG	265	0.0	17.432676	8
ATACACT	505	0.0	17.424456	4
ATTGTCG	140	8.37681E-8	17.285107	43
AGGACGT	850	0.0	17.08109	5
GATATAT	155	1.4391844E-8	17.032318	1
GGACGTG	845	0.0	16.92183	6
TAAGACC	195	4.9112714E-11	16.921827	4
GTCCTAC	875	0.0	16.845774	1
GTATCAA	3725	0.0	16.773212	1
GTGTATA	555	0.0	16.648706	1
TCCTAAA	565	0.0	16.352776	2
>>END_MODULE