FastQCFastQC Report
Thu 2 Feb 2017
SRR4064197_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064197_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057820
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21830.2063678130494791No Hit
TATCAACGCAGAGTACTTTTTTTTT16400.1550358284018075No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11800.11155016921593466No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10900.10304210546217693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG250.006037857418.996175
CGAACGA601.4623074E-615.83688316
GTCTAAC456.767712E-414.7754971
TTGCGCG852.688903E-713.41477118
AACCGCG852.698216E-713.4109647
AGACCTA500.001501273513.2973195
CGGTTCT2100.013.11950512
TCCAACG1352.7284841E-1112.66950518
ACTGATC905.394104E-712.6677088
CGGTTTC1352.7284841E-1112.667706513
GAGCGTT604.0921636E-412.6659117
CGAGCCG2000.012.35276815
GCCGGTT1001.4400575E-712.350430511
CCAACGT853.9300794E-612.29687419
GCGTAAC701.0899095E-412.21471111
GTATAAA1405.2750693E-1112.21241
TATACAG1405.2750693E-1112.21182255
CGTCGTA1950.012.18048910
GAACCGC951.0400599E-611.9987156
CGCGGTT2300.011.97924510
Produced by FastQC (version 0.11.5)