##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064197_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1057820
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.946178934034144	32.0	32.0	32.0	32.0	32.0
2	30.35733678697699	32.0	32.0	32.0	32.0	32.0
3	30.326957327333574	32.0	32.0	32.0	21.0	32.0
4	30.37069066570872	32.0	32.0	32.0	21.0	32.0
5	30.080704656746896	32.0	32.0	32.0	21.0	32.0
6	33.738112344255164	36.0	36.0	36.0	32.0	36.0
7	33.65027509406137	36.0	36.0	36.0	21.0	36.0
8	33.55240967272315	36.0	36.0	36.0	21.0	36.0
9	33.87710385509822	36.0	36.0	36.0	32.0	36.0
10	33.27331020400446	36.0	36.0	36.0	21.0	36.0
11	33.83264449528275	36.0	36.0	36.0	32.0	36.0
12	33.485727250382865	36.0	36.0	36.0	21.0	36.0
13	33.68610160518803	36.0	36.0	36.0	27.0	36.0
14	33.53017810213458	36.0	36.0	36.0	21.0	36.0
15	33.41558866347772	36.0	36.0	36.0	21.0	36.0
16	33.42333856421698	36.0	36.0	36.0	21.0	36.0
17	33.320663250836624	36.0	36.0	36.0	21.0	36.0
18	33.33078501068235	36.0	36.0	36.0	21.0	36.0
19	33.36526630239549	36.0	36.0	36.0	21.0	36.0
20	33.34319733035866	36.0	36.0	36.0	21.0	36.0
21	33.30196158136545	36.0	36.0	36.0	21.0	36.0
22	33.28449263579815	36.0	36.0	36.0	21.0	36.0
23	33.18717645724225	36.0	36.0	36.0	21.0	36.0
24	33.151220434478454	36.0	36.0	36.0	21.0	36.0
25	32.64602295286533	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	11.0
5	11.0
6	33.0
7	16.0
8	58.0
9	70.0
10	69.0
11	40.0
12	55.0
13	35.0
14	129.0
15	237.0
16	503.0
17	820.0
18	1278.0
19	1684.0
20	2480.0
21	3354.0
22	4718.0
23	6681.0
24	9253.0
25	12464.0
26	17232.0
27	22485.0
28	30105.0
29	40306.0
30	53810.0
31	74135.0
32	103062.0
33	136392.0
34	241492.0
35	294800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.76356015872355	17.952954126774575	11.583585200428205	25.699900514073665
2	16.613488905464592	19.81918615808874	38.478671699882675	25.08865323656399
3	18.990704353440655	24.050228444319238	28.84335233175806	28.115714870482044
4	12.160447725919134	15.612739768763175	36.839731893854164	35.387080611463524
5	13.948365578060477	37.292861032537175	34.2099789741185	14.54879441528385
6	33.45620804052501	36.48915541680809	17.070256032568256	12.984380510098637
7	29.621736310326373	30.733948423917052	21.1468747813491	18.497440484407473
8	27.525465528393166	33.948868471916995	19.497252030244688	19.02841396944515
9	27.374541547738616	13.89098530115514	18.62663874812978	40.107834402976465
10	15.271228088095928	27.053765149408587	32.441818893750316	25.233187868745166
11	36.982520786181574	20.739553944763756	23.41775803380834	18.86016723524633
12	24.545436487855447	23.503934363894846	29.898800915227913	22.051828233021794
13	29.61885015635316	19.75800730593953	25.519521916872712	25.103620620834604
14	23.013969960187175	20.1550893542074	25.64545680700948	31.18548387859595
15	24.786689379443676	28.280339480979627	22.44573478072113	24.487236358855572
16	25.10884874304886	25.90453471005041	24.959492092799763	24.02712445410097
17	23.36607628461302	26.302537302844716	25.9730899288231	24.358296483719172
18	23.982820356364083	25.293903402345176	27.37163750573522	23.351638735555525
19	25.56570289057149	24.82073461912492	25.601082971018606	24.012479519284984
20	25.560270000520326	24.73834830445539	25.516279025765463	24.185102669258825
21	26.41232956926538	24.399939078668925	24.5789191382848	24.608812213780897
22	25.458910874229474	25.005865280190108	25.214650334699375	24.32057351088104
23	23.986698391224344	24.768333467358552	25.78610521137354	25.45886293004357
24	24.631437544935103	25.14965754720551	25.777140802966663	24.441764104892723
25	24.685781209498746	24.80195181757809	25.83395927974223	24.678307693180937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	71.0
2	223.0
3	375.0
4	375.0
5	375.0
6	1048.0
7	1721.0
8	1721.0
9	1721.0
10	1879.0
11	2037.0
12	2037.0
13	2037.0
14	2413.5
15	2790.0
16	2790.0
17	2790.0
18	5013.0
19	7236.0
20	7236.0
21	7236.0
22	12106.0
23	16976.0
24	16976.0
25	16976.0
26	25901.0
27	34826.0
28	34826.0
29	34826.0
30	45394.5
31	55963.0
32	55963.0
33	55963.0
34	65700.5
35	75438.0
36	75438.0
37	75438.0
38	84237.5
39	93037.0
40	93037.0
41	93037.0
42	103985.0
43	114933.0
44	114933.0
45	114933.0
46	125734.5
47	136536.0
48	136536.0
49	136536.0
50	137454.5
51	138373.0
52	138373.0
53	138373.0
54	126732.5
55	115092.0
56	115092.0
57	115092.0
58	104398.0
59	93704.0
60	93704.0
61	93704.0
62	82464.5
63	71225.0
64	71225.0
65	71225.0
66	58855.5
67	46486.0
68	46486.0
69	46486.0
70	35170.5
71	23855.0
72	23855.0
73	23855.0
74	18313.5
75	12772.0
76	12772.0
77	12772.0
78	10151.5
79	7531.0
80	7531.0
81	7531.0
82	5391.5
83	3252.0
84	3252.0
85	3252.0
86	2489.0
87	1726.0
88	1726.0
89	1726.0
90	1278.0
91	830.0
92	830.0
93	830.0
94	598.5
95	367.0
96	367.0
97	367.0
98	517.5
99	668.0
100	668.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03630107201603297
2	0.004443099960295703
3	0.0012289425422094497
4	0.0028360212512525762
5	0.007184587169839859
6	0.009737006295967177
7	0.018717740258267
8	0.029494621013026794
9	0.04329659110245599
10	0.04896863360496115
11	0.04792875914616854
12	0.05643682289992627
13	0.056531356941634685
14	0.058894707984345165
15	0.052844529315006335
16	0.058516571817511485
17	0.05624775481650943
18	0.07137320148985649
19	0.06882078236372918
20	0.07420922274110907
21	0.06796997598835341
22	0.07108959936473125
23	0.07609990357527746
24	0.06995519086423022
25	0.07127866744814808
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1057820.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.373263042899666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.79295914521522	46.35355697052679
2	11.694263249846472	13.418760830527127
3	3.378859684239605	5.8156861634678325
4	1.291717786611561	2.9644025739382895
5	0.6984730461898742	2.0036838903713527
6	0.4449665435535249	1.5317509529151752
7	0.2782794476342038	1.1176059963985
8	0.20012558149989523	0.9185486103205396
9	0.16505458457372688	0.8522748087466466
>10	0.8913086272341691	9.37083099488201
>50	0.07820489371122163	3.1462374347756277
>100	0.07785331471102518	8.909403906515221
>500	0.007272920397907941	3.0194311234881845
>1k	6.611745816279946E-4	0.5778257431267787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2183	0.2063678130494791	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1640	0.1550358284018075	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1180	0.11155016921593466	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1090	0.10304210546217693	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.45340417084192E-5
2	0.0	0.0	0.0	0.0	9.45340417084192E-5
3	0.0	0.0	0.0	0.0	9.45340417084192E-5
4	0.0	0.0	0.0	0.0	9.45340417084192E-5
5	0.0	0.0	0.0	0.0	9.45340417084192E-5
6	0.0	0.0	0.0	0.0	9.45340417084192E-5
7	0.0	0.0	0.0	0.0	9.45340417084192E-5
8	0.0	0.0	0.0	0.0	9.45340417084192E-5
9	0.0	0.0	0.0	0.0	9.45340417084192E-5
10	0.0	0.0	0.0	0.0	9.45340417084192E-5
11	0.0	0.0	0.0	0.0	9.45340417084192E-5
12	0.0	0.0	0.0	0.0	9.45340417084192E-5
13	0.0	0.0	0.0	0.0	9.45340417084192E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	25	0.0060378574	18.99617	5
CGAACGA	60	1.4623074E-6	15.836883	16
GTCTAAC	45	6.767712E-4	14.775497	1
TTGCGCG	85	2.688903E-7	13.414771	18
AACCGCG	85	2.698216E-7	13.410964	7
AGACCTA	50	0.0015012735	13.297319	5
CGGTTCT	210	0.0	13.119505	12
TCCAACG	135	2.7284841E-11	12.669505	18
ACTGATC	90	5.394104E-7	12.667708	8
CGGTTTC	135	2.7284841E-11	12.6677065	13
GAGCGTT	60	4.0921636E-4	12.665911	7
CGAGCCG	200	0.0	12.352768	15
GCCGGTT	100	1.4400575E-7	12.3504305	11
CCAACGT	85	3.9300794E-6	12.296874	19
GCGTAAC	70	1.0899095E-4	12.214711	11
GTATAAA	140	5.2750693E-11	12.2124	1
TATACAG	140	5.2750693E-11	12.2118225	5
CGTCGTA	195	0.0	12.180489	10
GAACCGC	95	1.0400599E-6	11.998715	6
CGCGGTT	230	0.0	11.979245	10
>>END_MODULE