FastQCFastQC Report
Thu 2 Feb 2017
SRR4064197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057820
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG10960.10360930971242745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG250.002353094835.1986774
TAACGGC703.2111064E-522.00333236
GTATCAA19800.020.8881031
CGAATGC2950.020.88155743
ATACGAA2950.020.13578840
CGCAATA3150.019.55851736
GTCCTAC6400.019.2492751
ATTACTC808.989815E-519.2492753
GTAATAC1152.0216066E-719.1297153
TGTAGGA7750.019.0186392
GTCCTAA2900.018.9648041
TACGAAT3250.018.9540341
GATATAC4450.018.785811
TCTAGCG3300.018.66684728
CGTGATT2250.018.57795528
CGGTCCA3450.018.49118410
TTAACGG851.42896E-418.12039235
CTGTAGG8500.017.8581521
TCCTACA7650.017.8293932
TAGGACG6050.017.817514
Produced by FastQC (version 0.11.5)