##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064197_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1057820
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.945122043447846	32.0	32.0	32.0	32.0	32.0
2	30.57017923654308	32.0	32.0	32.0	32.0	32.0
3	31.031047815318296	32.0	32.0	32.0	32.0	32.0
4	31.29469853094099	32.0	32.0	32.0	32.0	32.0
5	31.10581951560757	32.0	32.0	32.0	32.0	32.0
6	34.707359475147	36.0	36.0	36.0	32.0	36.0
7	34.76478039742111	36.0	36.0	36.0	32.0	36.0
8	34.63278062430281	36.0	36.0	36.0	32.0	36.0
9	34.908480648881664	36.0	36.0	36.0	32.0	36.0
10	34.544415874156286	36.0	36.0	36.0	32.0	36.0
11	34.909320111172036	36.0	36.0	36.0	32.0	36.0
12	34.73593711595546	36.0	36.0	36.0	32.0	36.0
13	34.835582613299046	36.0	36.0	36.0	32.0	36.0
14	34.75162409483655	36.0	36.0	36.0	32.0	36.0
15	34.67282997107258	36.0	36.0	36.0	32.0	36.0
16	34.71426424155338	36.0	36.0	36.0	32.0	36.0
17	34.655380877654046	36.0	36.0	36.0	32.0	36.0
18	34.67118602408728	36.0	36.0	36.0	32.0	36.0
19	34.66298614130949	36.0	36.0	36.0	32.0	36.0
20	34.66879998487455	36.0	36.0	36.0	32.0	36.0
21	34.666115218090034	36.0	36.0	36.0	32.0	36.0
22	34.64105991567563	36.0	36.0	36.0	32.0	36.0
23	34.577834603240625	36.0	36.0	36.0	32.0	36.0
24	34.54405853547863	36.0	36.0	36.0	32.0	36.0
25	34.50725359702029	36.0	36.0	36.0	32.0	36.0
26	34.4255478247717	36.0	36.0	36.0	32.0	36.0
27	34.426226579191166	36.0	36.0	36.0	32.0	36.0
28	34.38773798945	36.0	36.0	36.0	32.0	36.0
29	34.35832561305326	36.0	36.0	36.0	32.0	36.0
30	34.33060255998185	36.0	36.0	36.0	32.0	36.0
31	34.34311414040196	36.0	36.0	36.0	32.0	36.0
32	34.28648257737611	36.0	36.0	36.0	32.0	36.0
33	34.262311168251685	36.0	36.0	36.0	32.0	36.0
34	34.23249607683727	36.0	36.0	36.0	32.0	36.0
35	34.19944224915392	36.0	36.0	36.0	32.0	36.0
36	34.14575636686771	36.0	36.0	36.0	32.0	36.0
37	34.14920402336882	36.0	36.0	36.0	32.0	36.0
38	34.0889830027793	36.0	36.0	36.0	32.0	36.0
39	34.07526516798699	36.0	36.0	36.0	32.0	36.0
40	34.06416119944792	36.0	36.0	36.0	32.0	36.0
41	34.034756385774514	36.0	36.0	36.0	32.0	36.0
42	33.959668941785935	36.0	36.0	36.0	32.0	36.0
43	33.94902251800873	36.0	36.0	36.0	32.0	36.0
44	33.85335028643814	36.0	36.0	36.0	32.0	36.0
45	33.81389083208864	36.0	36.0	36.0	32.0	36.0
46	33.78164337978106	36.0	36.0	36.0	32.0	36.0
47	33.75287572554877	36.0	36.0	36.0	32.0	36.0
48	33.70413491898432	36.0	36.0	36.0	27.0	36.0
49	33.684271426140555	36.0	36.0	36.0	27.0	36.0
50	32.89789567223157	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	2.0
20	4.0
21	26.0
22	105.0
23	333.0
24	777.0
25	1855.0
26	3931.0
27	7774.0
28	13393.0
29	22294.0
30	34896.0
31	54367.0
32	84760.0
33	143125.0
34	267149.0
35	423027.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.471913107846596	18.26463383851514	12.01044460473959	25.253008448898672
2	15.807584502015947	20.5980251745343	38.34857701961118	25.24581330383857
3	18.54731291459125	24.830996607170036	29.26899592463361	27.352694553605104
4	12.210962167476508	16.646688472518953	37.26219961808247	33.88014974192207
5	14.26792838101	37.23194872473577	33.447656501106046	15.052466393148173
6	33.61564349322191	37.02851146697926	16.769677260781606	12.586167779017224
7	29.698492557320826	30.96250961885693	20.89291352576719	18.44608429805505
8	27.470552646007828	34.261972736382376	19.251573991794444	19.01590062581536
9	27.46211512464431	13.730821224983691	18.299127442546393	40.5079362078256
10	15.086707278798123	27.124385051408	32.49698434126291	25.29192332853097
11	37.27064838124481	20.71148277729933	23.192909183896298	18.824959657559564
12	24.629426556503	23.413340643965892	29.738235238509386	22.218997561021723
13	29.705138034272473	19.71475217358012	25.469339214627862	25.110770577519553
14	23.10248369992163	20.05517022320496	25.62097224756267	31.22137382931074
15	24.771078460104405	28.230662912914333	22.365298841164467	24.63295978581679
16	25.021931980609104	25.942791562993943	24.868597185143727	24.166679271253226
17	23.41324610992418	26.34890624113743	25.775935414342705	24.46191223459568
18	24.12071275088224	25.39305002661141	27.09391132870809	23.392325893798265
19	25.535086815419273	24.87079547635276	25.593130771899542	24.000986936328427
20	25.586610135042516	24.745253186993946	25.478179076681652	24.18995760128189
21	26.51642056304475	24.380707492768146	24.553893857177968	24.548978087009132
22	25.41533609751394	25.14969493807063	25.191100926624426	24.243868037791
23	23.988315537215865	24.754895704825515	25.730680695584766	25.526108062373854
24	24.58504203936783	25.243283816308665	25.68939080257662	24.48228334174688
25	24.749221738937255	24.860016997429586	25.878539279849726	24.51222198378343
26	23.96383723161775	25.865001280993184	26.24362694075194	23.927534546637133
27	24.650563022501046	25.31753032077771	25.5414914164003	24.49041524032094
28	23.92542165861369	25.372215767446566	25.858419511678626	24.84394306226112
29	24.044620911325392	25.010115333711475	25.72329362828512	25.221970126678013
30	24.083090059293323	25.40641260285576	25.958411483543074	24.552085854307844
31	24.91715898573291	24.928692912968955	25.17109446570027	24.98305363559786
32	24.43285002963729	25.53278583914183	25.154544053681782	24.879820077539094
33	24.045003729512498	25.0367042643123	25.886598240275145	25.03169376590006
34	24.67656715444326	25.30646355194258	25.971527510580994	24.045441783033166
35	25.177870843929323	25.135423855396628	25.765038429177817	23.92166687149623
36	24.04922916349562	25.706241662703842	25.5806917911372	24.66383738266334
37	24.870342394397355	25.412134401476287	25.12549797407037	24.592025230055985
38	24.44710418067046	25.520326655945784	25.35611000341291	24.676459159970843
39	25.17721109722456	24.858146038204616	25.094963215343864	24.869679649226963
40	25.7285660670242	25.173544640417774	25.3789715796131	23.718917712944922
41	24.285719687874018	25.21540436651169	26.335677240403943	24.163198705210355
42	25.44443698661088	26.013826823432428	25.25564875746716	23.286087432489538
43	24.388000412175046	25.21301225275406	25.802252404275656	24.596734930795243
44	24.1430880781993	25.725697431485806	25.141566065739596	24.989648424575304
45	24.396838346721168	25.92897908018616	25.16513030333683	24.509052269755845
46	24.23994055654502	25.16628663694494	25.206463908520604	25.387308897989435
47	24.88513940200642	25.15494363795183	25.51578553304771	24.444131426994037
48	25.179167844417744	25.488297959835776	24.63946636768333	24.693067828063153
49	24.63112677441067	25.643968327194898	25.01937955183132	24.705525346563107
50	24.6920022688599	25.951975798827753	24.53053507279259	24.825486859519756
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	10.5
2	13.0
3	42.5
4	72.0
5	104.5
6	137.0
7	126.0
8	115.0
9	176.5
10	238.0
11	340.5
12	443.0
13	787.5
14	1132.0
15	1597.5
16	2063.0
17	2266.5
18	2470.0
19	2616.5
20	2763.0
21	3201.0
22	3639.0
23	4297.5
24	4956.0
25	6054.0
26	7152.0
27	9695.0
28	12238.0
29	14529.5
30	16821.0
31	19190.5
32	21560.0
33	25487.5
34	29415.0
35	34186.0
36	38957.0
37	43360.0
38	47763.0
39	50024.5
40	52286.0
41	54782.5
42	57279.0
43	57416.0
44	57553.0
45	62665.5
46	67778.0
47	71549.5
48	75321.0
49	76912.0
50	78503.0
51	75835.5
52	73168.0
53	71386.0
54	69604.0
55	69758.5
56	69913.0
57	68295.5
58	66678.0
59	60180.0
60	53682.0
61	47550.0
62	41418.0
63	36141.5
64	30865.0
65	26224.0
66	21583.0
67	18755.0
68	15927.0
69	14450.5
70	12974.0
71	10136.0
72	7298.0
73	6217.5
74	5137.0
75	4091.0
76	3045.0
77	2656.5
78	2268.0
79	1851.5
80	1435.0
81	1186.0
82	937.0
83	753.0
84	569.0
85	423.0
86	277.0
87	204.5
88	132.0
89	107.5
90	83.0
91	68.0
92	53.0
93	41.0
94	29.0
95	29.5
96	30.0
97	22.0
98	14.0
99	20.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00463216804371254
2	4.72670208542096E-4
3	9.45340417084192E-5
4	0.0
5	0.0
6	0.0
7	1.890680834168384E-4
8	0.0
9	9.45340417084192E-4
10	7.562723336673536E-4
11	9.45340417084192E-5
12	0.0
13	2.836021251252576E-4
14	2.836021251252576E-4
15	0.0032141574180862527
16	3.781361668336768E-4
17	0.0
18	2.836021251252576E-4
19	9.45340417084192E-5
20	4.72670208542096E-4
21	0.0
22	1.890680834168384E-4
23	4.72670208542096E-4
24	1.890680834168384E-4
25	0.0012289425422094497
26	0.004443099960295703
27	0.004443099960295703
28	0.0031196233763778336
29	0.001890680834168384
30	0.0026469531678357376
31	0.006711916961297764
32	0.0029305552929609954
33	0.0038758957100451874
34	0.0025524191261273184
35	0.0028360212512525762
36	0.006900985044714602
37	0.0032141574180862527
38	0.006711916961297764
39	0.0039704297517536066
40	0.0021742829592936415
41	0.0039704297517536066
42	0.0019852148758768033
43	0.0016070787090431264
44	9.45340417084192E-4
45	0.0017961467924599648
46	7.562723336673536E-4
47	0.0015125446673347072
48	0.0012289425422094497
49	3.781361668336768E-4
50	0.001890680834168384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1057820.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.06721410228614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.51906981719253	49.78142489945708
2	11.354350550216699	13.867571120849872
3	3.2488758628184606	5.951993937194537
4	1.2972895195735432	3.1688742737779796
5	0.6877751500564777	2.100025617136545
6	0.3971211774619826	1.455065038117372
7	0.26713809004356603	1.1419365257696368
8	0.1853328245873769	0.905420742340703
9	0.14557304817712252	0.8000766450499279
>10	0.7680937510360886	8.601834811824423
>50	0.06400334893574766	2.7545371099541818
>100	0.060562887916122916	7.373484455453816
>500	0.0043481037278092554	1.7961924914716536
>1k	4.6586825655099164E-4	0.30156233160233664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1096	0.10360930971242745	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.45340417084192E-5
2	0.0	0.0	0.0	0.0	9.45340417084192E-5
3	0.0	0.0	0.0	0.0	9.45340417084192E-5
4	0.0	0.0	0.0	0.0	9.45340417084192E-5
5	0.0	0.0	0.0	0.0	9.45340417084192E-5
6	0.0	0.0	0.0	0.0	9.45340417084192E-5
7	0.0	0.0	0.0	0.0	9.45340417084192E-5
8	0.0	0.0	0.0	0.0	9.45340417084192E-5
9	0.0	0.0	0.0	0.0	9.45340417084192E-5
10	0.0	0.0	0.0	9.45340417084192E-5	9.45340417084192E-5
11	0.0	0.0	0.0	9.45340417084192E-5	9.45340417084192E-5
12	0.0	0.0	0.0	9.45340417084192E-5	2.836021251252576E-4
13	0.0	0.0	0.0	9.45340417084192E-5	2.836021251252576E-4
14	0.0	0.0	0.0	9.45340417084192E-5	2.836021251252576E-4
15	9.45340417084192E-5	0.0	0.0	9.45340417084192E-5	2.836021251252576E-4
16	9.45340417084192E-5	0.0	0.0	9.45340417084192E-5	2.836021251252576E-4
17	9.45340417084192E-5	0.0	0.0	9.45340417084192E-5	2.836021251252576E-4
18	9.45340417084192E-5	0.0	0.0	9.45340417084192E-5	2.836021251252576E-4
19	9.45340417084192E-5	0.0	0.0	9.45340417084192E-5	2.836021251252576E-4
20	9.45340417084192E-5	0.0	0.0	9.45340417084192E-5	3.781361668336768E-4
21	9.45340417084192E-5	0.0	0.0	9.45340417084192E-5	4.72670208542096E-4
22	9.45340417084192E-5	0.0	0.0	1.890680834168384E-4	4.72670208542096E-4
23	9.45340417084192E-5	0.0	0.0	3.781361668336768E-4	4.72670208542096E-4
24	9.45340417084192E-5	0.0	0.0	3.781361668336768E-4	5.672042502505152E-4
25	9.45340417084192E-5	0.0	0.0	3.781361668336768E-4	5.672042502505152E-4
26	9.45340417084192E-5	0.0	0.0	5.672042502505152E-4	5.672042502505152E-4
27	9.45340417084192E-5	0.0	0.0	0.0010398744587926112	6.617382919589344E-4
28	9.45340417084192E-5	0.0	0.0	0.0014180106256262881	6.617382919589344E-4
29	9.45340417084192E-5	0.0	0.0	0.0031196233763778336	6.617382919589344E-4
30	9.45340417084192E-5	0.0	0.0	0.006333780794464086	6.617382919589344E-4
31	9.45340417084192E-5	0.0	0.0	0.0142746402979713	6.617382919589344E-4
32	9.45340417084192E-5	0.0	0.0	0.02268817001002061	8.508063753757728E-4
33	9.45340417084192E-5	0.0	0.0	0.031857972055737274	8.508063753757728E-4
34	9.45340417084192E-5	0.0	0.0	0.04206764856024654	8.508063753757728E-4
35	9.45340417084192E-5	0.0	0.0	0.05473521014917472	8.508063753757728E-4
36	9.45340417084192E-5	0.0	0.0	0.07175133765669017	8.508063753757728E-4
37	9.45340417084192E-5	0.0	0.0	0.10285303737876009	8.508063753757728E-4
38	9.45340417084192E-5	0.0	0.0	0.1443534816887561	8.508063753757728E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	25	0.0023530948	35.198677	4
TAACGGC	70	3.2111064E-5	22.003332	36
GTATCAA	1980	0.0	20.888103	1
CGAATGC	295	0.0	20.881557	43
ATACGAA	295	0.0	20.135788	40
CGCAATA	315	0.0	19.558517	36
GTCCTAC	640	0.0	19.249275	1
ATTACTC	80	8.989815E-5	19.249275	3
GTAATAC	115	2.0216066E-7	19.129715	3
TGTAGGA	775	0.0	19.018639	2
GTCCTAA	290	0.0	18.964804	1
TACGAAT	325	0.0	18.95403	41
GATATAC	445	0.0	18.78581	1
TCTAGCG	330	0.0	18.666847	28
CGTGATT	225	0.0	18.577955	28
CGGTCCA	345	0.0	18.491184	10
TTAACGG	85	1.42896E-4	18.120392	35
CTGTAGG	850	0.0	17.858152	1
TCCTACA	765	0.0	17.829393	2
TAGGACG	605	0.0	17.81751	4
>>END_MODULE