FastQCFastQC Report
Thu 2 Feb 2017
SRR4064196_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064196_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647481
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40750.2473473138688701No Hit
TATCAACGCAGAGTACTTTTTTTTT28190.1711097123426613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT1800.013.194939
CGGTCCA3150.012.96951910
AGAACCG1251.03682396E-1012.91733555
CCGTCGT2000.012.8254729
ACCGTCG2150.012.81442458
CGAACGA1155.304173E-912.39439716
GATAACG1155.318725E-912.39213811
CGTTATT1405.2750693E-1112.2084292
GCGTTAT1405.2750693E-1112.2076871
CGTCGTA2150.011.93139610
ACGAACG1209.980795E-911.87760315
TAAATCC1850.011.8076584
ATACCGT2350.011.722416
GCGTAAC1302.6120688E-911.69309311
GTCCTAC1552.5465852E-1111.638871
CTTATAC907.488472E-611.60730653
CTTACAC1657.2759576E-1211.5113773
CGAGCCG2150.011.49088915
GGTCCAA3500.011.40076511
CGCCGGT3250.011.4000737
Produced by FastQC (version 0.11.5)