##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064196_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1647481
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28155043973193	32.0	32.0	32.0	32.0	32.0
2	30.88035977349663	32.0	32.0	32.0	32.0	32.0
3	30.901549092220183	32.0	32.0	32.0	32.0	32.0
4	30.926975182111356	32.0	32.0	32.0	32.0	32.0
5	30.8298572183837	32.0	32.0	32.0	32.0	32.0
6	34.49725611403106	36.0	36.0	36.0	32.0	36.0
7	34.440273969775674	36.0	36.0	36.0	32.0	36.0
8	34.399891713470446	36.0	36.0	36.0	32.0	36.0
9	34.542982893277674	36.0	36.0	36.0	32.0	36.0
10	34.23186671045068	36.0	36.0	36.0	32.0	36.0
11	34.522148055121725	36.0	36.0	36.0	32.0	36.0
12	34.33909040529147	36.0	36.0	36.0	32.0	36.0
13	34.41842060697513	36.0	36.0	36.0	32.0	36.0
14	34.31910231438177	36.0	36.0	36.0	32.0	36.0
15	34.24529933880876	36.0	36.0	36.0	32.0	36.0
16	34.254001715346035	36.0	36.0	36.0	32.0	36.0
17	34.18904740024316	36.0	36.0	36.0	32.0	36.0
18	34.17744119659043	36.0	36.0	36.0	32.0	36.0
19	34.191162750890605	36.0	36.0	36.0	32.0	36.0
20	34.17201108844351	36.0	36.0	36.0	32.0	36.0
21	34.17381019872156	36.0	36.0	36.0	32.0	36.0
22	34.149456655342306	36.0	36.0	36.0	32.0	36.0
23	34.0963076357178	36.0	36.0	36.0	32.0	36.0
24	34.08335877621654	36.0	36.0	36.0	32.0	36.0
25	33.68169223195897	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	9.0
5	23.0
6	79.0
7	23.0
8	103.0
9	101.0
10	136.0
11	39.0
12	96.0
13	94.0
14	150.0
15	279.0
16	455.0
17	589.0
18	729.0
19	1076.0
20	1576.0
21	2223.0
22	3510.0
23	5664.0
24	8196.0
25	11953.0
26	17557.0
27	23562.0
28	31686.0
29	42729.0
30	56670.0
31	78363.0
32	111078.0
33	157939.0
34	342453.0
35	748338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.17829037491863	17.264570723508438	11.749664817495555	25.807474084077377
2	16.680466239405995	19.581897226771495	37.35172035347949	26.385916180343017
3	18.59879013901383	23.554169989960315	28.87963424953049	28.967405621495367
4	12.736012548082673	15.539603368412363	35.76063949574578	35.96374458775918
5	14.784577112615779	36.36917705021131	33.622834206874444	15.223411630298461
6	34.13763085238904	35.311872448818534	16.95441443935321	13.596082259439218
7	30.249451912492997	30.5807632972313	20.733584240336448	18.436200549939258
8	28.312414270907272	32.57319951448264	19.387419902690077	19.72696631192001
9	27.358232185919746	14.175900853166567	18.679665680647652	39.786201280266035
10	16.019913369433453	26.578651371488682	31.585174172138775	25.816261086939097
11	36.7895210870026	21.265311253668962	22.60888154438616	19.33628611494228
12	24.55587536996467	23.613296802011124	28.83856755267878	22.992260275345426
13	29.39770569009992	19.47922190010939	25.25156080978073	25.87151160000996
14	23.662166881785744	19.49683395338243	25.327704806013212	31.513294358818616
15	25.16494068330356	27.0345417342595	22.31914830297704	25.481369279459898
16	25.552594831413856	25.831147209404453	23.63255139036267	24.98370656881902
17	24.042000617084494	25.955326969750715	25.140483901140147	24.862188512024645
18	24.67592187621491	25.216193822408833	25.962940284581293	24.144944016794962
19	25.56643956439127	24.91240687915794	25.510919375452545	24.010234180998246
20	25.83008189197743	24.327524172439077	24.921925922416143	24.92046801316735
21	26.627207433108822	24.182326442545996	24.66153502249954	24.528931101845643
22	25.917133596077306	24.13911661653149	24.968290916866316	24.97545887052489
23	24.55056135407801	24.12191416242788	25.567930191743077	25.759594291751032
24	24.71403943370392	24.820526714153633	25.29610510470743	25.16932874743501
25	24.87213068547672	24.46246291486151	25.383121979429134	25.28228442023263
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	141.0
1	141.0
2	340.5
3	540.0
4	540.0
5	540.0
6	1471.5
7	2403.0
8	2403.0
9	2403.0
10	2646.5
11	2890.0
12	2890.0
13	2890.0
14	3623.0
15	4356.0
16	4356.0
17	4356.0
18	7589.0
19	10822.0
20	10822.0
21	10822.0
22	16929.5
23	23037.0
24	23037.0
25	23037.0
26	33748.5
27	44460.0
28	44460.0
29	44460.0
30	55586.5
31	66713.0
32	66713.0
33	66713.0
34	83663.0
35	100613.0
36	100613.0
37	100613.0
38	117493.5
39	134374.0
40	134374.0
41	134374.0
42	155327.0
43	176280.0
44	176280.0
45	176280.0
46	196355.0
47	216430.0
48	216430.0
49	216430.0
50	222148.5
51	227867.0
52	227867.0
53	227867.0
54	213988.5
55	200110.0
56	200110.0
57	200110.0
58	182064.5
59	164019.0
60	164019.0
61	164019.0
62	142381.5
63	120744.0
64	120744.0
65	120744.0
66	97964.0
67	75184.0
68	75184.0
69	75184.0
70	56671.5
71	38159.0
72	38159.0
73	38159.0
74	28648.0
75	19137.0
76	19137.0
77	19137.0
78	15058.5
79	10980.0
80	10980.0
81	10980.0
82	7516.5
83	4053.0
84	4053.0
85	4053.0
86	3054.5
87	2056.0
88	2056.0
89	2056.0
90	1488.0
91	920.0
92	920.0
93	920.0
94	643.5
95	367.0
96	367.0
97	367.0
98	596.5
99	826.0
100	826.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03745111476247678
2	0.005280789277691215
3	0.0011532758192658974
4	0.0035205261851274767
5	0.009893892554754804
6	0.012443239102605735
7	0.02367250365861579
8	0.0350838643966152
9	0.04807339204518899
10	0.05760309223596508
11	0.0545074571421461
12	0.06312667642297544
13	0.06434065096957112
14	0.06713279242674118
15	0.05948475278318839
16	0.0674969847907199
17	0.062458990422347815
18	0.07726947989081513
19	0.07484153079762378
20	0.0781799608007619
21	0.07435594097898549
22	0.07678389007217686
23	0.08327865389646376
24	0.0773908773454747
25	0.077026684981496
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1647481.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.92134540874275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.52688533524726	41.8035396262586
2	13.016225589754088	14.037047918864925
3	3.871231394278133	6.262260155041199
4	1.7695359710738088	3.816630412378634
5	0.9418596528909301	2.5393169835045195
6	0.60289404081496	1.9505314691793663
7	0.39798974242016316	1.5022099659121921
8	0.30318235811955757	1.307840052320144
9	0.2287451787279598	1.1100823013497423
>10	1.1923029734159358	11.450670227628045
>50	0.07604666368718795	2.819479319460052
>100	0.06392121501474747	7.141201651903895
>500	0.006799914485378708	2.4372537042879943
>1k	0.0023799700698825476	1.8219362119108957
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4075	0.2473473138688701	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2819	0.1711097123426613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.0698727329784075E-5	0.0
6	0.0	0.0	0.0	6.0698727329784075E-5	0.0
7	0.0	0.0	0.0	6.0698727329784075E-5	0.0
8	0.0	0.0	0.0	6.0698727329784075E-5	0.0
9	0.0	0.0	0.0	6.0698727329784075E-5	6.0698727329784075E-5
10	0.0	0.0	0.0	6.0698727329784075E-5	1.2139745465956815E-4
11	0.0	0.0	0.0	6.0698727329784075E-5	1.2139745465956815E-4
12	0.0	0.0	0.0	6.0698727329784075E-5	2.427949093191363E-4
13	0.0	0.0	0.0	6.0698727329784075E-5	2.427949093191363E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTAT	180	0.0	13.19493	9
CGGTCCA	315	0.0	12.969519	10
AGAACCG	125	1.03682396E-10	12.9173355	5
CCGTCGT	200	0.0	12.825472	9
ACCGTCG	215	0.0	12.8144245	8
CGAACGA	115	5.304173E-9	12.394397	16
GATAACG	115	5.318725E-9	12.392138	11
CGTTATT	140	5.2750693E-11	12.208429	2
GCGTTAT	140	5.2750693E-11	12.207687	1
CGTCGTA	215	0.0	11.931396	10
ACGAACG	120	9.980795E-9	11.877603	15
TAAATCC	185	0.0	11.807658	4
ATACCGT	235	0.0	11.72241	6
GCGTAAC	130	2.6120688E-9	11.693093	11
GTCCTAC	155	2.5465852E-11	11.63887	1
CTTATAC	90	7.488472E-6	11.6073065	3
CTTACAC	165	7.2759576E-12	11.511377	3
CGAGCCG	215	0.0	11.490889	15
GGTCCAA	350	0.0	11.400765	11
CGCCGGT	325	0.0	11.400073	7
>>END_MODULE