FastQCFastQC Report
Thu 2 Feb 2017
SRR4064196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647481
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27600.022.3176541
TAGACCG550.004487244419.9977235
TATACTA901.0033067E-519.553335
ATACCGT3700.018.4303346
CTAGACA2050.018.2418254
ACCGTCG3400.018.1155858
TACCGTC3750.017.5979987
GTATTAG3400.017.469661
GGTATCA14250.016.9815541
AACGCAG37000.016.8251136
CGTCGTA3900.016.35909810
CCGTCGT3800.016.2101579
TCAACGC39250.015.5803934
ATCAACG39550.015.51833
GTATTAA3000.015.3991821
TCTAGAC2001.2787496E-915.3987143
TATCAAC40100.015.3603142
CAACGCA40950.015.0947415
AATAGCG3500.015.0839975
TAGCGTA3550.014.8715487
Produced by FastQC (version 0.11.5)