##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064196_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1647481
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.354516379855063	32.0	32.0	32.0	32.0	32.0
2	31.43947031862583	32.0	32.0	32.0	32.0	32.0
3	31.504674712485304	32.0	32.0	32.0	32.0	32.0
4	31.58564802871778	32.0	32.0	32.0	32.0	32.0
5	31.53848511758254	32.0	32.0	32.0	32.0	32.0
6	35.14495341676171	36.0	36.0	36.0	36.0	36.0
7	35.15983735169025	36.0	36.0	36.0	36.0	36.0
8	35.11160189404309	36.0	36.0	36.0	36.0	36.0
9	35.21228530101409	36.0	36.0	36.0	36.0	36.0
10	35.079564498771155	36.0	36.0	36.0	36.0	36.0
11	35.21036540026865	36.0	36.0	36.0	36.0	36.0
12	35.1273774932761	36.0	36.0	36.0	36.0	36.0
13	35.15673625371097	36.0	36.0	36.0	36.0	36.0
14	35.11014026868899	36.0	36.0	36.0	36.0	36.0
15	35.08432995585382	36.0	36.0	36.0	36.0	36.0
16	35.091452951505964	36.0	36.0	36.0	36.0	36.0
17	35.05808807506733	36.0	36.0	36.0	36.0	36.0
18	35.059377923023085	36.0	36.0	36.0	36.0	36.0
19	35.06126322549395	36.0	36.0	36.0	36.0	36.0
20	35.04455407983461	36.0	36.0	36.0	36.0	36.0
21	35.052608193964	36.0	36.0	36.0	36.0	36.0
22	35.03467900388532	36.0	36.0	36.0	36.0	36.0
23	34.984232898588814	36.0	36.0	36.0	36.0	36.0
24	34.95955643798016	36.0	36.0	36.0	36.0	36.0
25	34.93216492329805	36.0	36.0	36.0	36.0	36.0
26	34.876459273278414	36.0	36.0	36.0	32.0	36.0
27	34.87765261025772	36.0	36.0	36.0	32.0	36.0
28	34.8559352126064	36.0	36.0	36.0	32.0	36.0
29	34.82440950760586	36.0	36.0	36.0	32.0	36.0
30	34.81333563179181	36.0	36.0	36.0	32.0	36.0
31	34.80636256199616	36.0	36.0	36.0	32.0	36.0
32	34.76863223308797	36.0	36.0	36.0	32.0	36.0
33	34.74142463554966	36.0	36.0	36.0	32.0	36.0
34	34.74131598482774	36.0	36.0	36.0	32.0	36.0
35	34.704655167495105	36.0	36.0	36.0	32.0	36.0
36	34.678331950414	36.0	36.0	36.0	32.0	36.0
37	34.6689321454997	36.0	36.0	36.0	32.0	36.0
38	34.63990662107788	36.0	36.0	36.0	32.0	36.0
39	34.64384536149431	36.0	36.0	36.0	32.0	36.0
40	34.617839598757136	36.0	36.0	36.0	32.0	36.0
41	34.60250224433544	36.0	36.0	36.0	32.0	36.0
42	34.53327291786673	36.0	36.0	36.0	32.0	36.0
43	34.52443700412933	36.0	36.0	36.0	32.0	36.0
44	34.470741695958864	36.0	36.0	36.0	32.0	36.0
45	34.44179750783165	36.0	36.0	36.0	32.0	36.0
46	34.42207406337311	36.0	36.0	36.0	32.0	36.0
47	34.39218904497229	36.0	36.0	36.0	32.0	36.0
48	34.34980008874154	36.0	36.0	36.0	32.0	36.0
49	34.33471038512735	36.0	36.0	36.0	32.0	36.0
50	33.75256952887469	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	1.0
20	13.0
21	31.0
22	149.0
23	477.0
24	1194.0
25	2689.0
26	5645.0
27	10334.0
28	17257.0
29	26384.0
30	38367.0
31	54950.0
32	80036.0
33	129518.0
34	276056.0
35	1004378.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.3645858310544	18.16200105018105	12.280258720903763	26.193154397860784
2	15.957571435074888	20.376879770557977	36.86423162718706	26.801317167180077
3	17.821564623703168	24.15664932296434	29.23162461850734	28.79016143482515
4	12.369065257808739	16.165163665013434	35.82275000440065	35.64302107277717
5	14.292607926889596	36.92619216852881	33.39334414175338	15.38785576282822
6	34.24843123472662	35.692399394466925	16.66569548290292	13.393473887903543
7	30.230127364294173	30.75820693313845	20.702479363925125	18.309186338642252
8	28.35514140089191	32.77522809091952	19.25651252610807	19.6131179820805
9	27.52961214374075	14.012307386009788	18.399772741895738	40.058307728353725
10	15.938292887232466	26.903839850436427	31.618734294004103	25.539132968327
11	37.03480467137689	21.121288270570812	22.534598295578704	19.309308762473595
12	24.635048237366448	23.681287398071476	28.73992854532807	22.943735819234004
13	29.54494550138303	19.519470121816866	25.283570656393156	25.652013720406952
14	23.758239103502987	19.312051929038248	25.43467928878001	31.49502967867876
15	25.20684032958481	27.134780375936472	22.20490880815051	25.453470486328207
16	25.75019529934063	25.92100255482494	23.614740411212093	24.71406173462234
17	24.090778588645332	26.035080222472974	25.18414476403673	24.68999642484496
18	24.85532431996057	25.121003133274012	25.93825228622173	24.085420260543692
19	25.64254155283126	24.933094827800744	25.476834027220953	23.947529592147042
20	25.981229445806125	24.295497227877345	24.979999684365275	24.743273641951255
21	26.77645447807896	24.179338031819487	24.66177151663661	24.382435973464943
22	25.948328293306496	24.11619457133874	25.04537238130039	24.89010475405438
23	24.6767152423361	24.01715606532222	25.54374481020526	25.762383882136426
24	24.911242854966364	24.832455524309047	25.2715522500217	24.98474937070289
25	25.126664914471213	24.35687244037325	25.375229823904355	25.141232821251176
26	24.90900791309436	25.13190513999063	25.48616119014493	24.472925756770078
27	25.32216326661928	24.74075485968924	24.87132590618188	25.065755967509602
28	24.7587419251842	24.677766136580782	25.696944165554818	24.8665477726802
29	24.807747288066764	24.829174459054983	25.441275286945025	24.921802965933225
30	24.766664886100415	24.98015073302675	25.62922783316418	24.62395654770865
31	25.243083841313506	24.678791822621942	24.895136137877614	25.182988198186933
32	25.099989255973103	24.783131513566307	24.657845233810058	25.459033996650533
33	24.809578267763186	24.502244754752347	25.08571102515728	25.602465952327186
34	25.336752811205365	24.562028620422783	25.353263426256127	24.747955142115728
35	25.763140882644837	24.468400877728104	25.21204174975037	24.556416489876685
36	24.941815643305915	24.95523112878259	24.94661122408717	25.15634200382432
37	25.575737415541617	24.76494908064725	24.98626631273715	24.673047191073984
38	24.989695703032286	24.71258751064575	25.231899796706386	25.065816989615573
39	25.428034483135388	24.657372099545107	24.71163896871096	25.202954448608544
40	25.586453213033995	24.667333557518727	25.230326214030686	24.51588701541659
41	24.6590459356236	25.127774311111327	25.36304900463508	24.850130748629994
42	25.65727136704796	25.119593554907414	24.931849428360714	24.291285649683907
43	25.26480253773398	24.36978513512365	25.14716647627187	25.218245850870495
44	24.874913427284078	24.863077017394666	24.906780684678647	25.35522887064261
45	24.916629134096286	25.004886315766473	25.017572526886877	25.060912023250363
46	24.87656861352592	24.802819346466165	25.027466290818552	25.293145749189367
47	25.078591203733776	24.716941161759788	25.260751622660244	24.943716011846192
48	25.785414123584243	25.173435439040986	24.390464495584975	24.650685941789792
49	25.019909242987453	25.24516290373881	24.603636107597318	25.131291745676414
50	25.00696524660615	25.36885457354178	24.64999256433369	24.974187615518385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	27.0
2	30.0
3	113.5
4	197.0
5	225.5
6	254.0
7	241.0
8	228.0
9	314.0
10	400.0
11	651.5
12	903.0
13	1701.0
14	2499.0
15	3309.5
16	4120.0
17	4364.0
18	4608.0
19	4503.5
20	4399.0
21	4861.5
22	5324.0
23	6322.0
24	7320.0
25	8450.5
26	9581.0
27	11507.5
28	13434.0
29	15874.0
30	18314.0
31	21223.5
32	24133.0
33	27705.0
34	31277.0
35	36755.5
36	42234.0
37	49705.5
38	57177.0
39	63584.5
40	69992.0
41	77022.5
42	84053.0
43	87783.5
44	91514.0
45	100919.5
46	110325.0
47	117273.5
48	124222.0
49	128539.5
50	132857.0
51	130774.5
52	128692.0
53	126677.5
54	124663.0
55	123883.0
56	123103.0
57	118395.5
58	113688.0
59	103767.0
60	93846.0
61	81691.5
62	69537.0
63	59952.5
64	50368.0
65	42397.5
66	34427.0
67	29267.5
68	24108.0
69	21271.5
70	18435.0
71	14181.5
72	9928.0
73	8428.0
74	6928.0
75	5407.5
76	3887.0
77	3264.5
78	2642.0
79	2128.5
80	1615.0
81	1295.0
82	975.0
83	797.0
84	619.0
85	451.0
86	283.0
87	192.0
88	101.0
89	86.5
90	72.0
91	58.0
92	44.0
93	41.5
94	39.0
95	36.0
96	33.0
97	25.5
98	18.0
99	14.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008862014190148475
2	3.6419236397870443E-4
3	3.034936366489204E-4
4	0.0
5	0.0
6	4.2489109130848854E-4
7	1.2139745465956816E-4
8	1.2139745465956816E-4
9	9.104809099467611E-4
10	0.0012746732739254657
11	6.069872732978408E-5
12	1.8209618198935222E-4
13	4.8558981863827265E-4
14	1.2139745465956816E-4
15	0.004552404549733806
16	6.069872732978408E-4
17	0.0
18	1.8209618198935222E-4
19	0.0
20	4.2489109130848854E-4
21	0.0
22	1.8209618198935222E-4
23	0.0010318783646063292
24	4.8558981863827265E-4
25	0.0014567694559148177
26	0.0051593918230316465
27	0.006191270187637976
28	0.004552404549733806
29	0.002427949093191363
30	0.003945417276435965
31	0.0064947638242868954
32	0.0032777312758083404
33	0.0051593918230316465
34	0.0033991287304679083
35	0.0027921414571700676
36	0.007951533280201714
37	0.004370308367744454
38	0.006676860006276248
39	0.004066814731095533
40	0.0021244554565424427
41	0.0027314427298402834
42	0.001578166910574386
43	0.0016388656379041702
44	0.0013353720012552496
45	0.0013960707285850338
46	4.2489109130848854E-4
47	0.0019423592745530906
48	0.0023065516385317947
49	3.034936366489204E-4
50	9.711796372765453E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1647481.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.85137231330813
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63956336488747	44.707670954132034
2	12.455815371213639	14.162603942693858
3	3.782729762412744	6.451601342506755
4	1.6135876312799602	3.6693868474418396
5	0.889369048586006	2.5280925452547827
6	0.5502426542203318	1.8769229998645771
7	0.3757143553727881	1.4951913690527057
8	0.2656689117610039	1.2082913771676989
9	0.2035813355190126	1.0416490471468698
>10	1.0967763824530248	10.889158379208677
>50	0.06730665091745014	2.658793739742702
>100	0.0524314484858029	6.154477982010097
>500	0.006028845401270242	2.33748107245005
>1k	0.0011842374895352263	0.818678401327325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.0698727329784075E-5
2	0.0	0.0	0.0	0.0	6.0698727329784075E-5
3	0.0	0.0	0.0	0.0	6.0698727329784075E-5
4	0.0	0.0	0.0	0.0	1.2139745465956815E-4
5	0.0	0.0	0.0	6.0698727329784075E-5	1.2139745465956815E-4
6	0.0	0.0	0.0	6.0698727329784075E-5	3.6419236397870443E-4
7	0.0	0.0	0.0	6.0698727329784075E-5	3.6419236397870443E-4
8	0.0	0.0	0.0	6.0698727329784075E-5	3.6419236397870443E-4
9	0.0	0.0	0.0	6.0698727329784075E-5	3.6419236397870443E-4
10	0.0	0.0	0.0	6.0698727329784075E-5	3.6419236397870443E-4
11	0.0	0.0	0.0	6.0698727329784075E-5	3.6419236397870443E-4
12	0.0	0.0	0.0	6.0698727329784075E-5	4.2489109130848854E-4
13	0.0	0.0	0.0	6.0698727329784075E-5	4.2489109130848854E-4
14	0.0	0.0	0.0	6.0698727329784075E-5	5.462885459680567E-4
15	0.0	0.0	0.0	6.0698727329784075E-5	6.676860006276248E-4
16	0.0	0.0	0.0	6.0698727329784075E-5	7.283847279574089E-4
17	0.0	0.0	0.0	6.0698727329784075E-5	7.283847279574089E-4
18	0.0	0.0	0.0	6.0698727329784075E-5	7.89083455287193E-4
19	0.0	0.0	0.0	6.0698727329784075E-5	8.497821826169771E-4
20	0.0	0.0	0.0	1.2139745465956815E-4	9.104809099467611E-4
21	0.0	0.0	0.0	1.2139745465956815E-4	9.104809099467611E-4
22	0.0	0.0	0.0	2.427949093191363E-4	9.104809099467611E-4
23	0.0	0.0	0.0	4.855898186382726E-4	9.104809099467611E-4
24	0.0	0.0	0.0	8.497821826169771E-4	9.104809099467611E-4
25	0.0	0.0	0.0	0.0012139745465956815	9.104809099467611E-4
26	0.0	0.0	0.0	0.001578166910574386	9.104809099467611E-4
27	0.0	0.0	0.0	0.0020030580018828743	9.104809099467611E-4
28	0.0	0.0	0.0	0.0027921414571700676	9.104809099467611E-4
29	0.0	0.0	0.0	0.005462885459680567	9.104809099467611E-4
30	0.0	0.0	0.0	0.01044018110072286	9.104809099467611E-4
31	0.0	0.0	0.0	0.022701324021339245	9.104809099467611E-4
32	0.0	0.0	0.0	0.03720831985315764	9.711796372765452E-4
33	0.0	0.0	0.0	0.05329348259555042	9.711796372765452E-4
34	0.0	0.0	0.0	0.06986423515658147	9.711796372765452E-4
35	0.0	0.0	0.0	0.09286905281456964	9.711796372765452E-4
36	0.0	0.0	0.0	0.12346121138878081	9.711796372765452E-4
37	0.0	0.0	0.0	0.16607171797428924	0.0010318783646063292
38	0.0	0.0	0.0	0.2256778682121372	0.0010925770919361134
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2760	0.0	22.317654	1
TAGACCG	55	0.0044872444	19.997723	5
TATACTA	90	1.0033067E-5	19.55333	5
ATACCGT	370	0.0	18.430334	6
CTAGACA	205	0.0	18.241825	4
ACCGTCG	340	0.0	18.115585	8
TACCGTC	375	0.0	17.597998	7
GTATTAG	340	0.0	17.46966	1
GGTATCA	1425	0.0	16.981554	1
AACGCAG	3700	0.0	16.825113	6
CGTCGTA	390	0.0	16.359098	10
CCGTCGT	380	0.0	16.210157	9
TCAACGC	3925	0.0	15.580393	4
ATCAACG	3955	0.0	15.5183	3
GTATTAA	300	0.0	15.399182	1
TCTAGAC	200	1.2787496E-9	15.398714	3
TATCAAC	4010	0.0	15.360314	2
CAACGCA	4095	0.0	15.094741	5
AATAGCG	350	0.0	15.083997	5
TAGCGTA	355	0.0	14.871548	7
>>END_MODULE