FastQCFastQC Report
Thu 2 Feb 2017
SRR4064195_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064195_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3475
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT160.46043165467625896No Hit
TATCAACGCAGAGTACTTTTTTTTT140.4028776978417266No Hit
GAGTACTTTTTTTTTTTTTTTTTTT120.34532374100719426No Hit
GTACTTTTTTTTTTTTTTTTTTTTT80.23021582733812948No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60.17266187050359713No Hit
TTCTTATACATCTCCTCCATCATGT50.14388489208633093No Hit
GCTAAGAGCATCGAGGGGGCGCCGA50.14388489208633093No Hit
GTATCTGATCGTCTTCGAACCTCCG40.11510791366906474No Hit
CTCTTAATCATGGCCTCAGTTCCGA40.11510791366906474No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG40.11510791366906474No Hit
GCGCAAGACGGACCAGAGCGAAAGC40.11510791366906474No Hit
GAATAACGCCGCCGCATCGCCAGTC40.11510791366906474No Hit
CTCCGGAATCGAACCCTGATTCCCC40.11510791366906474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG250.004919993719.06
ACGCAGA250.004919993719.07
GTATCAA250.004919993719.01
TCAACGC305.7997426E-419.04
CAGAGTA250.004919993719.010
AGAGTAC250.004919993719.011
GCAGAGT250.004919993719.09
CGCAGAG250.004919993719.08
TATCAAC250.004919993719.02
ATCAACG305.7997426E-419.03
CAACGCA305.7997426E-419.05
Produced by FastQC (version 0.11.5)