##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064195_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3475
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.281438848920864	32.0	32.0	32.0	21.0	32.0
2	29.064460431654677	32.0	32.0	32.0	14.0	32.0
3	29.246906474820143	32.0	32.0	32.0	14.0	32.0
4	29.18302158273381	32.0	32.0	32.0	14.0	32.0
5	29.03453237410072	32.0	32.0	32.0	14.0	32.0
6	32.41956834532374	36.0	32.0	36.0	21.0	36.0
7	32.46676258992806	36.0	32.0	36.0	21.0	36.0
8	32.30503597122302	36.0	32.0	36.0	21.0	36.0
9	32.73208633093525	36.0	32.0	36.0	21.0	36.0
10	31.755971223021582	36.0	32.0	36.0	14.0	36.0
11	32.627913669064746	36.0	32.0	36.0	21.0	36.0
12	32.233093525179854	36.0	32.0	36.0	14.0	36.0
13	32.65122302158274	36.0	32.0	36.0	21.0	36.0
14	32.229928057553956	36.0	32.0	36.0	14.0	36.0
15	32.193093525179854	36.0	32.0	36.0	14.0	36.0
16	31.994244604316545	36.0	32.0	36.0	14.0	36.0
17	31.909928057553955	36.0	32.0	36.0	14.0	36.0
18	31.951079136690648	36.0	32.0	36.0	14.0	36.0
19	31.862158273381294	36.0	32.0	36.0	14.0	36.0
20	31.95971223021583	36.0	32.0	36.0	14.0	36.0
21	31.789928057553958	36.0	32.0	36.0	14.0	36.0
22	31.80978417266187	36.0	32.0	36.0	14.0	36.0
23	31.83251798561151	36.0	32.0	36.0	14.0	36.0
24	31.703884892086332	36.0	32.0	36.0	14.0	36.0
25	31.118273381294966	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	4.0
15	4.0
16	7.0
17	10.0
18	16.0
19	17.0
20	37.0
21	35.0
22	54.0
23	67.0
24	57.0
25	87.0
26	103.0
27	125.0
28	143.0
29	170.0
30	196.0
31	282.0
32	332.0
33	378.0
34	595.0
35	755.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.82014388489209	18.61870503597122	11.53956834532374	27.02158273381295
2	15.428900402993667	19.976971790443294	38.543465745538285	26.050662061024752
3	17.496402877697843	25.151079136690647	28.086330935251798	29.26618705035971
4	11.942446043165468	15.194244604316548	35.884892086330936	36.97841726618705
5	14.70503597122302	37.20863309352518	33.697841726618705	14.388489208633093
6	33.89928057553957	36.69064748201439	16.892086330935253	12.51798561151079
7	29.3321819228555	30.19573978123201	21.81922855497985	18.65284974093264
8	27.325079182263174	33.54448603512813	19.29167866397927	19.838756118629426
9	26.605240426144544	13.56176216527498	19.234091563489777	40.5989058450907
10	15.22740356937248	27.979274611398964	32.843983880253305	23.949337938975248
11	36.62539591131587	21.220846530377198	24.07140800460697	18.082349553699974
12	22.948459545061905	22.80449179383818	31.788079470198678	22.458969190901236
13	28.64133563615429	20.581462291306853	24.29476108232585	26.482440990213014
14	21.934369602763386	19.919401266551525	27.605066206102475	30.541162924582615
15	24.52504317789292	26.770293609671846	23.43120322394934	25.273459988485897
16	25.302245250431778	25.15831894070236	25.734024179620036	23.805411629245825
17	23.668298301180535	24.848833861215088	27.44025338324215	24.042614454362223
18	23.553124100201554	25.48229196659948	26.80679527785776	24.157788655341204
19	24.553828439838803	25.129533678756477	26.94300518134715	23.37363270005757
20	23.639504750935792	24.4745177080334	27.267492081773682	24.618485459257126
21	25.432027649769584	25.316820276497698	25.57603686635945	23.67511520737327
22	23.35156924848834	24.647279009501872	27.12352433054996	24.877627411459834
23	23.322775698243596	23.95623380362799	27.267492081773682	25.453498416354737
24	24.179620034542314	25.331030512377662	26.59758203799655	23.89176741508348
25	24.35934350705442	25.19435646415203	26.720414627123525	23.725885401670027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	6.5
7	10.0
8	10.0
9	10.0
10	14.5
11	19.0
12	19.0
13	19.0
14	18.5
15	18.0
16	18.0
17	18.0
18	23.5
19	29.0
20	29.0
21	29.0
22	47.0
23	65.0
24	65.0
25	65.0
26	103.5
27	142.0
28	142.0
29	142.0
30	159.0
31	176.0
32	176.0
33	176.0
34	205.5
35	235.0
36	235.0
37	235.0
38	274.5
39	314.0
40	314.0
41	314.0
42	350.5
43	387.0
44	387.0
45	387.0
46	400.0
47	413.0
48	413.0
49	413.0
50	412.0
51	411.0
52	411.0
53	411.0
54	397.0
55	383.0
56	383.0
57	383.0
58	351.0
59	319.0
60	319.0
61	319.0
62	275.0
63	231.0
64	231.0
65	231.0
66	197.5
67	164.0
68	164.0
69	164.0
70	117.0
71	70.0
72	70.0
73	70.0
74	54.0
75	38.0
76	38.0
77	38.0
78	30.0
79	22.0
80	22.0
81	22.0
82	16.5
83	11.0
84	11.0
85	11.0
86	7.5
87	4.0
88	4.0
89	4.0
90	4.0
91	4.0
92	4.0
93	4.0
94	3.0
95	2.0
96	2.0
97	2.0
98	3.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.028776978417266185
3	0.0
4	0.0
5	0.0
6	0.0
7	0.028776978417266185
8	0.05755395683453237
9	0.05755395683453237
10	0.028776978417266185
11	0.05755395683453237
12	0.05755395683453237
13	0.028776978417266185
14	0.028776978417266185
15	0.028776978417266185
16	0.028776978417266185
17	0.05755395683453237
18	0.05755395683453237
19	0.028776978417266185
20	0.05755395683453237
21	0.08633093525179857
22	0.05755395683453237
23	0.05755395683453237
24	0.028776978417266185
25	0.05755395683453237
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3475.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.36690647482014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.34459867229933	92.83453237410072
2	1.9915509957754978	3.798561151079137
3	0.27157513578756787	0.776978417266187
4	0.18105009052504525	0.6906474820143885
5	0.06035003017501509	0.28776978417266186
6	0.030175015087507546	0.17266187050359713
7	0.0	0.0
8	0.030175015087507546	0.23021582733812948
9	0.0	0.0
>10	0.09052504526252263	1.20863309352518
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	16	0.46043165467625896	No Hit
TATCAACGCAGAGTACTTTTTTTTT	14	0.4028776978417266	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	12	0.34532374100719426	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	8	0.23021582733812948	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	6	0.17266187050359713	No Hit
TTCTTATACATCTCCTCCATCATGT	5	0.14388489208633093	No Hit
GCTAAGAGCATCGAGGGGGCGCCGA	5	0.14388489208633093	No Hit
GTATCTGATCGTCTTCGAACCTCCG	4	0.11510791366906474	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	4	0.11510791366906474	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	4	0.11510791366906474	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	4	0.11510791366906474	No Hit
GAATAACGCCGCCGCATCGCCAGTC	4	0.11510791366906474	No Hit
CTCCGGAATCGAACCCTGATTCCCC	4	0.11510791366906474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAG	25	0.0049199937	19.0	6
ACGCAGA	25	0.0049199937	19.0	7
GTATCAA	25	0.0049199937	19.0	1
TCAACGC	30	5.7997426E-4	19.0	4
CAGAGTA	25	0.0049199937	19.0	10
AGAGTAC	25	0.0049199937	19.0	11
GCAGAGT	25	0.0049199937	19.0	9
CGCAGAG	25	0.0049199937	19.0	8
TATCAAC	25	0.0049199937	19.0	2
ATCAACG	30	5.7997426E-4	19.0	3
CAACGCA	30	5.7997426E-4	19.0	5
>>END_MODULE