FastQCFastQC Report
Thu 2 Feb 2017
SRR4064194_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064194_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764339
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38550.5043573597579084No Hit
TATCAACGCAGAGTACTTTTTTTTT28290.3701237278223406No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28030.36672209582397336No Hit
GTACATGGGGTGGTATCAACGCAAA17100.22372271989261308No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16910.2212369118938063No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15130.19794881590498456No Hit
GGTATCAACGCAGAGTACTTTTTTT13800.1805481599133369No Hit
GTATCAACGCAGAGTACATGGGGTG10870.14221438393173708No Hit
GATTAAGAGGGACGGCCGGGGGCAT9960.13030867193745185No Hit
GTATCTGATCGTCTTCGAACCTCCG9220.120627103942099No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9110.11918795194278979No Hit
CTATTGGAGCTGGAATTACCGCGGC8900.11644047994410858No Hit
GAATAGGACCGCGGTTCTATTTTGT8820.11539382394461097No Hit
GAATAACGCCGCCGCATCGCCAGTC8180.10702057594863011No Hit
GTCCTATTCCATTATTCCTAGCTGC8090.10584308794919532No Hit
CTTTAATATACGCTATTGGAGCTGG7750.1013947999513305No Hit
GTACATGGGTGGTATCAACGCAAAA7740.10126396795139331No Hit
ATCAGATACCGTCGTAGTTCCGACC7710.10087147195158169No Hit
GTCTTGCGCCGGTCCAAGAATTTCA7670.10034814395183289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG250.006037735418.9954175
GTAAACC250.006037735418.9954174
GTTAGGC504.543919E-617.10483416
ACGAACG601.4601173E-615.83780915
ATAACGA601.4621128E-615.83573412
CGTTATT653.3866236E-614.6070782
CGTCTTA400.005265963714.25402815
CGAACGA759.623436E-713.93727216
CGTTAGG551.953407E-413.82208815
TCGTTAG551.9544929E-413.82118314
GCCTTAT551.9653811E-413.8121361
GTATTAG1257.2759576E-1213.6740141
TAACGAA707.2347284E-613.57437613
CGGTCCA1351.8189894E-1213.37239910
TTTAGGC500.001502537313.2950523
GGTCCAA1600.013.0627711
TTAGGCT604.080309E-412.66941717
TGGCGAG1800.012.66858818
GATAACG751.475938E-512.66692911
TCCATTA1457.2759576E-1212.4501648
Produced by FastQC (version 0.11.5)