##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064194_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 764339 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.174777160396108 32.0 32.0 32.0 32.0 32.0 2 30.743434523163153 32.0 32.0 32.0 32.0 32.0 3 30.765265150672672 32.0 32.0 32.0 32.0 32.0 4 30.793097042019312 32.0 32.0 32.0 32.0 32.0 5 30.68464385567137 32.0 32.0 32.0 32.0 32.0 6 34.326223050243414 36.0 36.0 36.0 32.0 36.0 7 34.24050715716456 36.0 36.0 36.0 32.0 36.0 8 34.19336577094718 36.0 36.0 36.0 32.0 36.0 9 34.34424254159477 36.0 36.0 36.0 32.0 36.0 10 34.009924915515235 36.0 36.0 36.0 32.0 36.0 11 34.36385425838535 36.0 36.0 36.0 32.0 36.0 12 34.13296979481618 36.0 36.0 36.0 32.0 36.0 13 34.236693404366385 36.0 36.0 36.0 32.0 36.0 14 34.12726028633892 36.0 36.0 36.0 32.0 36.0 15 34.04537907917822 36.0 36.0 36.0 32.0 36.0 16 34.05143267581531 36.0 36.0 36.0 32.0 36.0 17 33.956033906421105 36.0 36.0 36.0 32.0 36.0 18 33.964585085936996 36.0 36.0 36.0 32.0 36.0 19 33.97442626897227 36.0 36.0 36.0 32.0 36.0 20 33.94338637698718 36.0 36.0 36.0 32.0 36.0 21 33.91910657443883 36.0 36.0 36.0 32.0 36.0 22 33.90232737044688 36.0 36.0 36.0 32.0 36.0 23 33.85114458375145 36.0 36.0 36.0 32.0 36.0 24 33.84910491287243 36.0 36.0 36.0 32.0 36.0 25 33.449110931144425 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 5.0 5 8.0 6 34.0 7 19.0 8 53.0 9 62.0 10 64.0 11 22.0 12 51.0 13 43.0 14 107.0 15 176.0 16 246.0 17 324.0 18 415.0 19 599.0 20 888.0 21 1327.0 22 2051.0 23 3196.0 24 4623.0 25 6805.0 26 9489.0 27 12286.0 28 16816.0 29 22387.0 30 29403.0 31 40012.0 32 55788.0 33 78707.0 34 163053.0 35 315278.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.39660103003056 17.63825902585547 11.879773052987023 25.08536689112695 2 16.229667776172832 20.406147292176936 37.95217609746907 25.412008834181165 3 18.506762821099898 25.284237354217964 28.73590588309177 27.47309394159037 4 12.360298831625911 15.732621580248852 36.20520469443027 35.70187489369497 5 14.405006614163337 37.09583929218639 33.05289106322512 15.44626303042515 6 32.93109471973495 35.875392392011236 17.53192458222273 13.661588306031092 7 29.48457223919114 30.9424164618618 21.288844220053342 18.284167078893713 8 28.241436357068437 32.08349257372612 19.953693766327593 19.721377302877848 9 28.098050611834562 13.863799395757736 19.013210599896325 39.02493939251138 10 16.503179201723853 26.48483840857946 30.936637207067687 26.075345182629007 11 36.46406742340837 21.326265988906766 23.219603895330344 18.990062692354517 12 25.44038228651851 23.80990410107027 28.81550093280529 21.93421267960593 13 29.700109451016743 19.6550983749928 25.51989232952611 25.124899844464345 14 24.012563564093032 20.1239598007845 25.620322703968075 30.243153931154392 15 24.699120894945963 27.44836976088081 23.01802067173314 24.834488672440088 16 25.10320177534548 25.719597535988058 24.650986193939474 24.52621449472699 17 23.321000552487632 26.018239947212557 25.739508626400532 24.92125087389928 18 24.21210998314821 24.723425038332262 27.405308510875102 23.65915646764443 19 25.49411125441083 24.49691978343557 26.169598491643153 23.839370470510445 20 25.923016085018567 23.8382751161463 25.6644039743771 24.574304824458025 21 27.224923860925397 23.67119965851985 24.90932706937232 24.19454941118243 22 26.11172144145041 24.10583695494191 25.400319234047792 24.382122369559895 23 24.03727797943609 24.274815757632897 26.38502595368668 25.30288030924433 24 24.628170161171 24.946741161389667 26.23830891566707 24.186779761772264 25 24.645283275806985 24.569468959340483 26.227170593996657 24.558077170855878 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 635.0 1 635.0 2 790.5 3 946.0 4 946.0 5 946.0 6 2166.5 7 3387.0 8 3387.0 9 3387.0 10 3130.0 11 2873.0 12 2873.0 13 2873.0 14 2462.5 15 2052.0 16 2052.0 17 2052.0 18 3712.5 19 5373.0 20 5373.0 21 5373.0 22 8818.0 23 12263.0 24 12263.0 25 12263.0 26 18648.0 27 25033.0 28 25033.0 29 25033.0 30 30948.0 31 36863.0 32 36863.0 33 36863.0 34 43349.5 35 49836.0 36 49836.0 37 49836.0 38 55101.0 39 60366.0 40 60366.0 41 60366.0 42 69133.0 43 77900.0 44 77900.0 45 77900.0 46 88328.0 47 98756.0 48 98756.0 49 98756.0 50 100976.5 51 103197.0 52 103197.0 53 103197.0 54 93632.0 55 84067.0 56 84067.0 57 84067.0 58 77509.5 59 70952.0 60 70952.0 61 70952.0 62 62908.0 63 54864.0 64 54864.0 65 54864.0 66 45401.0 67 35938.0 68 35938.0 69 35938.0 70 27082.0 71 18226.0 72 18226.0 73 18226.0 74 13945.0 75 9664.0 76 9664.0 77 9664.0 78 7940.0 79 6216.0 80 6216.0 81 6216.0 82 4266.0 83 2316.0 84 2316.0 85 2316.0 86 1813.0 87 1310.0 88 1310.0 89 1310.0 90 937.5 91 565.0 92 565.0 93 565.0 94 409.0 95 253.0 96 253.0 97 253.0 98 370.5 99 488.0 100 488.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037156287982164984 2 0.004579119997802022 3 0.0017008159991836085 4 0.0037941279981788183 5 0.009158239995604044 6 0.015176511992715273 7 0.025773903987628525 8 0.03859543998147419 9 0.05324862397444066 10 0.0612293759706099 11 0.05887439997174029 12 0.06724764796772113 13 0.06894846396690474 14 0.07104177596589996 15 0.06463100796897711 16 0.07117260796583715 17 0.06816347196728154 18 0.0813775039609388 19 0.07771420796269718 20 0.08464830395936881 21 0.07915335996200638 22 0.0832091519600596 23 0.08778827195786162 24 0.08059251196131559 25 0.0825549919603736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 764339.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.046640519968335 #Duplication Level Percentage of deduplicated Percentage of total 1 76.17599378950696 38.88528571224302 2 14.207502417981901 14.50490537234606 3 4.538454523378666 6.950185697134049 4 1.8258346027482713 3.7281089046164078 5 0.9016520870410817 2.3013154980632646 6 0.5338760609963261 1.6351547620737688 7 0.3454830590736518 1.234502466559018 8 0.2285945885647624 0.9335188629820391 9 0.1753785169345118 0.8057235697993083 >10 0.8392183914162847 8.04295604463086 >50 0.10191067685860034 3.681472374405286 >100 0.11374850651152994 11.521657270462564 >500 0.010293982489580723 3.559292880491327 >1k 0.0020587964979161444 2.2159205841930945 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3855 0.5043573597579084 No Hit TATCAACGCAGAGTACTTTTTTTTT 2829 0.3701237278223406 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2803 0.36672209582397336 No Hit GTACATGGGGTGGTATCAACGCAAA 1710 0.22372271989261308 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1691 0.2212369118938063 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1513 0.19794881590498456 No Hit GGTATCAACGCAGAGTACTTTTTTT 1380 0.1805481599133369 No Hit GTATCAACGCAGAGTACATGGGGTG 1087 0.14221438393173708 No Hit GATTAAGAGGGACGGCCGGGGGCAT 996 0.13030867193745185 No Hit GTATCTGATCGTCTTCGAACCTCCG 922 0.120627103942099 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 911 0.11918795194278979 No Hit CTATTGGAGCTGGAATTACCGCGGC 890 0.11644047994410858 No Hit GAATAGGACCGCGGTTCTATTTTGT 882 0.11539382394461097 No Hit GAATAACGCCGCCGCATCGCCAGTC 818 0.10702057594863011 No Hit GTCCTATTCCATTATTCCTAGCTGC 809 0.10584308794919532 No Hit CTTTAATATACGCTATTGGAGCTGG 775 0.1013947999513305 No Hit GTACATGGGTGGTATCAACGCAAAA 774 0.10126396795139331 No Hit ATCAGATACCGTCGTAGTTCCGACC 771 0.10087147195158169 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 767 0.10034814395183289 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.3083199993720065E-4 5 1.3083199993720065E-4 0.0 0.0 0.0 1.3083199993720065E-4 6 1.3083199993720065E-4 0.0 0.0 0.0 1.3083199993720065E-4 7 1.3083199993720065E-4 0.0 0.0 0.0 1.3083199993720065E-4 8 1.3083199993720065E-4 0.0 0.0 0.0 1.3083199993720065E-4 9 1.3083199993720065E-4 0.0 0.0 0.0 1.3083199993720065E-4 10 1.3083199993720065E-4 0.0 0.0 0.0 1.3083199993720065E-4 11 1.3083199993720065E-4 0.0 0.0 0.0 1.3083199993720065E-4 12 1.3083199993720065E-4 0.0 0.0 0.0 1.3083199993720065E-4 13 1.3083199993720065E-4 0.0 0.0 0.0 1.3083199993720065E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 25 0.0060377354 18.995417 5 GTAAACC 25 0.0060377354 18.995417 4 GTTAGGC 50 4.543919E-6 17.104834 16 ACGAACG 60 1.4601173E-6 15.837809 15 ATAACGA 60 1.4621128E-6 15.835734 12 CGTTATT 65 3.3866236E-6 14.607078 2 CGTCTTA 40 0.0052659637 14.254028 15 CGAACGA 75 9.623436E-7 13.937272 16 CGTTAGG 55 1.953407E-4 13.822088 15 TCGTTAG 55 1.9544929E-4 13.821183 14 GCCTTAT 55 1.9653811E-4 13.812136 1 GTATTAG 125 7.2759576E-12 13.674014 1 TAACGAA 70 7.2347284E-6 13.574376 13 CGGTCCA 135 1.8189894E-12 13.372399 10 TTTAGGC 50 0.0015025373 13.295052 3 GGTCCAA 160 0.0 13.06277 11 TTAGGCT 60 4.080309E-4 12.669417 17 TGGCGAG 180 0.0 12.668588 18 GATAACG 75 1.475938E-5 12.666929 11 TCCATTA 145 7.2759576E-12 12.450164 8 >>END_MODULE