FastQCFastQC Report
Thu 2 Feb 2017
SRR4064194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764339
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.15464342392577116No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11480.15019513592790634No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10590.1385510879334955No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9520.12455206394021501No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9310.1218045919415338No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8750.11447799994505055No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC8600.11251551994599254No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8160.10675891194875572No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG7970.1042731039499489No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7850.10270311995070251No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA7760.1015256319512677No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7700.10074063995164448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG554.950236E-628.00557935
TAACGGC609.731788E-625.67178336
CGTCTTA602.8713804E-422.00006715
GTATAGA500.002582474821.9957491
GTACTAC703.2181575E-521.9957471
GTATATA1205.984475E-1021.9957471
TCACGTC1057.736526E-820.95244442
GGCCGCG856.1084284E-620.70594440
ACTTCGC654.936311E-420.3037668
TAGGACG2900.019.7203254
TCTAGAC808.996405E-519.2462793
TAGGACC5400.019.1444474
ATGCGGC3050.018.75783735
TAAGGCC1303.5901394E-818.6117864
CGCCTCG951.596716E-518.52515811
TGCGGCG3100.018.4552936
GTCCTAA1801.4551915E-1118.329791
GTAATGG600.00741793618.329791
TTAGGAC2650.018.260623
CGGCGTT3150.018.15878341
Produced by FastQC (version 0.11.5)