##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064194_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	764339
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2293759706099	32.0	32.0	32.0	32.0	32.0
2	31.420657587798086	32.0	32.0	32.0	32.0	32.0
3	31.487483956726006	32.0	32.0	32.0	32.0	32.0
4	31.57008212324636	32.0	32.0	32.0	32.0	32.0
5	31.51281041527385	32.0	32.0	32.0	32.0	32.0
6	35.08928760667714	36.0	36.0	36.0	36.0	36.0
7	35.11343788554555	36.0	36.0	36.0	36.0	36.0
8	35.073578608444684	36.0	36.0	36.0	36.0	36.0
9	35.170925466317954	36.0	36.0	36.0	36.0	36.0
10	35.02642806398732	36.0	36.0	36.0	36.0	36.0
11	35.171348053677754	36.0	36.0	36.0	36.0	36.0
12	35.07652494508327	36.0	36.0	36.0	36.0	36.0
13	35.1177108586635	36.0	36.0	36.0	36.0	36.0
14	35.05656783181285	36.0	36.0	36.0	36.0	36.0
15	35.03829975966162	36.0	36.0	36.0	36.0	36.0
16	35.03649820302248	36.0	36.0	36.0	36.0	36.0
17	34.99655650176165	36.0	36.0	36.0	36.0	36.0
18	34.99405368560286	36.0	36.0	36.0	36.0	36.0
19	34.99297824656337	36.0	36.0	36.0	36.0	36.0
20	34.979424051369875	36.0	36.0	36.0	36.0	36.0
21	34.97360071905267	36.0	36.0	36.0	36.0	36.0
22	34.96782186961545	36.0	36.0	36.0	36.0	36.0
23	34.91985885843847	36.0	36.0	36.0	32.0	36.0
24	34.88498166389521	36.0	36.0	36.0	32.0	36.0
25	34.85092347767156	36.0	36.0	36.0	32.0	36.0
26	34.79960593401619	36.0	36.0	36.0	32.0	36.0
27	34.79820341497686	36.0	36.0	36.0	32.0	36.0
28	34.76349630203352	36.0	36.0	36.0	32.0	36.0
29	34.73127368876899	36.0	36.0	36.0	32.0	36.0
30	34.70253251502278	36.0	36.0	36.0	32.0	36.0
31	34.68535558175103	36.0	36.0	36.0	32.0	36.0
32	34.63410476241563	36.0	36.0	36.0	32.0	36.0
33	34.592777550275464	36.0	36.0	36.0	32.0	36.0
34	34.56950253748664	36.0	36.0	36.0	32.0	36.0
35	34.51364904839345	36.0	36.0	36.0	32.0	36.0
36	34.47835580809039	36.0	36.0	36.0	32.0	36.0
37	34.45551777418135	36.0	36.0	36.0	32.0	36.0
38	34.39299316141136	36.0	36.0	36.0	32.0	36.0
39	34.3910489978923	36.0	36.0	36.0	32.0	36.0
40	34.35148932607129	36.0	36.0	36.0	32.0	36.0
41	34.32084978000599	36.0	36.0	36.0	32.0	36.0
42	34.22760843029075	36.0	36.0	36.0	32.0	36.0
43	34.21483137717688	36.0	36.0	36.0	32.0	36.0
44	34.117381162023655	36.0	36.0	36.0	32.0	36.0
45	34.074994210684004	36.0	36.0	36.0	32.0	36.0
46	34.02073425535005	36.0	36.0	36.0	32.0	36.0
47	33.95618697986103	36.0	36.0	36.0	32.0	36.0
48	33.88080158149722	36.0	36.0	36.0	32.0	36.0
49	33.8477141687131	36.0	36.0	36.0	32.0	36.0
50	33.248324369160805	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	7.0
21	26.0
22	108.0
23	291.0
24	703.0
25	1593.0
26	3480.0
27	6207.0
28	10245.0
29	14958.0
30	21155.0
31	29129.0
32	41613.0
33	65147.0
34	131645.0
35	438025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.481190621675765	18.60539362243596	12.415982247181308	25.49743350870697
2	15.531409656760452	21.226425585639806	37.237860362275704	26.00430439532404
3	17.772896284104675	25.841577940649294	29.091056574447432	27.2944692007986
4	12.08272769020029	16.508643416075852	36.08320391868006	35.325424975043795
5	13.898283353328825	37.521963421989454	33.024744256148125	15.555008968533595
6	32.9123579158904	36.35129053191267	17.13291013376316	13.603441418433778
7	29.236749283037827	31.178957945196878	21.32491469720123	18.25937807456407
8	28.28901835442127	32.42265539243713	19.664049590561255	19.624276662580346
9	28.044111951936866	13.690588333804335	19.029788258444018	39.23551145581478
10	16.41232559843418	26.842402738091497	30.974921761338063	25.770349902136257
11	36.64682817440952	21.211137989818653	23.08295141292018	19.05908242285164
12	25.238408611885564	24.030567588465328	28.897779650129067	21.83324414952004
13	29.761713184471127	19.605118724740983	25.623584546485752	25.00958354430214
14	23.974833156492082	20.163827830321363	25.69056400366853	30.17077500951803
15	24.915282179033944	27.509152141431002	23.101470361192302	24.47409531834275
16	25.01596165017296	25.660445984205815	24.79262938094964	24.530962984671582
17	23.362670228785916	25.979571891529808	25.828460931602336	24.82929694808194
18	24.3263433882481	24.539992882711267	27.65105922002889	23.482604509011743
19	25.571768547725547	24.323369604324782	26.4508287553036	23.654033092646067
20	25.947130512144547	23.760523854069223	25.889694963595804	24.402650670190425
21	27.14162171497202	23.6710412526379	25.16004024392318	24.0272967884669
22	26.079072451026185	24.021995533383837	25.728441773720228	24.170490241869754
23	24.062255506100737	24.038705592057923	26.62448615395887	25.274552747882474
24	24.770258106382535	24.70955182014193	26.456035042180403	24.064155031295137
25	24.65230440732784	24.322991215698178	26.475236747008445	24.549467629965537
26	24.158824945570256	25.51459659186066	26.87978877072517	23.44678969184391
27	24.780934461557383	24.943181000406923	25.929221255866718	24.346663282168972
28	23.882395579694123	24.967584336332628	26.64652599598844	24.503494087984812
29	24.11040095249933	24.6854331713125	26.493219329979524	24.71094654620865
30	23.762851662571403	25.126848428230875	26.834035938861543	24.276263970336178
31	24.64639274807462	24.915801566470314	25.574472762101152	24.863332923353912
32	24.367847974732236	25.124886496557608	25.4357616309954	25.071503897714752
33	23.794763767679807	24.79896374412854	26.316712242735086	25.089560245456568
34	24.832558985423244	24.74895296212608	26.411783869925255	24.006704182525425
35	25.32284358800579	24.774630970480256	26.160863114318683	23.74166232719527
36	24.036296310834594	25.569010748889454	25.809110714215617	24.585582226060332
37	25.156581800007068	25.4474373182153	25.341719253117574	24.05426162866006
38	24.26864468653584	25.381607974187	25.97184749812238	24.377899841154782
39	25.064569055525464	24.92744976435893	25.46846910498261	24.539512075132997
40	25.34736941265131	25.294511694116046	25.78187555932363	23.576243333909012
41	24.01305761443403	25.656511471205867	26.370885968297998	23.95954494606211
42	25.4815070670754	26.19102756833207	25.489749698751435	22.837715665841095
43	24.42685171738422	25.176657756914217	25.736101244094513	24.660389281607053
44	23.987900466685073	25.889050105517665	25.151538543058145	24.971510884739125
45	24.25381872894384	26.087462793968534	25.177509567265233	24.481208909822392
46	24.12766736880667	25.600853034683972	25.176036528724506	25.095443067784856
47	24.624275339090936	25.517222430830945	25.5025689400947	24.355933289983422
48	25.23625544764569	26.126719009421436	24.31033557889944	24.326689964033434
49	24.41995253383992	26.32003809852761	24.570541150858134	24.689468216774344
50	24.508294203150736	26.636697024448896	24.435419825755663	24.419588946644705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	357.0
1	208.0
2	59.0
3	172.0
4	285.0
5	305.0
6	325.0
7	296.0
8	267.0
9	400.0
10	533.0
11	821.5
12	1110.0
13	1887.0
14	2664.0
15	3599.5
16	4535.0
17	4737.0
18	4939.0
19	4871.0
20	4803.0
21	4997.0
22	5191.0
23	5544.5
24	5898.0
25	5711.0
26	5524.0
27	6631.5
28	7739.0
29	8986.0
30	10233.0
31	11816.0
32	13399.0
33	15807.5
34	18216.0
35	20619.5
36	23023.0
37	26385.5
38	29748.0
39	31274.0
40	32800.0
41	35253.0
42	37706.0
43	37877.5
44	38049.0
45	42416.5
46	46784.0
47	49875.5
48	52967.0
49	54970.5
50	56974.0
51	54683.5
52	52393.0
53	51924.0
54	51455.0
55	52756.0
56	54057.0
57	52641.0
58	51225.0
59	46624.0
60	42023.0
61	37344.0
62	32665.0
63	28183.0
64	23701.0
65	19944.0
66	16187.0
67	13915.5
68	11644.0
69	10645.0
70	9646.0
71	7406.0
72	5166.0
73	4430.0
74	3694.0
75	2891.0
76	2088.0
77	1876.5
78	1665.0
79	1394.0
80	1123.0
81	856.0
82	589.0
83	529.0
84	469.0
85	336.0
86	203.0
87	144.0
88	85.0
89	69.0
90	53.0
91	43.0
92	33.0
93	25.0
94	17.0
95	15.5
96	14.0
97	10.0
98	6.0
99	8.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008634911995855242
2	5.233279997488025E-4
3	1.3083199993720062E-4
4	0.0
5	0.0
6	3.924959998116019E-4
7	3.924959998116019E-4
8	0.0
9	0.001439151999309207
10	0.001439151999309207
11	0.0
12	0.0
13	0.001046655999497605
14	0.0
15	0.004840783997676424
16	9.158239995604046E-4
17	0.0
18	3.924959998116019E-4
19	0.0
20	5.233279997488025E-4
21	0.0
22	2.6166399987440124E-4
23	6.541599996860032E-4
24	3.924959998116019E-4
25	0.0027474719986812137
26	0.006672431996797233
27	0.009158239995604044
28	0.006279935996985631
29	0.004448287997864822
30	0.005364111997425226
31	0.009550735995415647
32	0.005887439997174029
33	0.006410767996922832
34	0.005233279997488026
35	0.004840783997676424
36	0.009681567995352847
37	0.005494943997362427
38	0.009550735995415647
39	0.005364111997425226
40	0.003009135998555615
41	0.004448287997864822
42	0.002616639998744013
43	0.001046655999497605
44	0.0015699839992464077
45	0.0018316479991208091
46	0.0011774879994348058
47	0.00209331199899521
48	0.0023549759988696116
49	6.541599996860032E-4
50	0.0013083199993720064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	764339.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.543303775176014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5030705734063	43.04776592367611
2	13.671358257156593	15.187048093930189
3	4.217470347048256	7.02756709946695
4	1.7225934957663467	3.827141352659703
5	0.856896127980275	2.379742097009026
6	0.47988294029127204	1.5992570357473666
7	0.28555817837341085	1.1102591252816179
8	0.19989022517806104	0.8882050799002703
9	0.13243609579550403	0.662034446961119
>10	0.7454040148918197	7.63996881290213
>50	0.08573360219349413	3.4076672429571153
>100	0.09189069611571786	10.092987657435604
>500	0.007104950729978953	2.6845529227441216
>1k	7.104950729978953E-4	0.4458031093286862
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1182	0.15464342392577116	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1148	0.15019513592790634	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1059	0.1385510879334955	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	952	0.12455206394021501	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	931	0.1218045919415338	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	875	0.11447799994505055	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	860	0.11251551994599254	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	816	0.10675891194875572	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	797	0.1042731039499489	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	785	0.10270311995070251	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	776	0.1015256319512677	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	770	0.10074063995164448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	3.924959998116019E-4	0.0
17	0.0	0.0	0.0	3.924959998116019E-4	0.0
18	0.0	0.0	0.0	5.233279997488026E-4	1.3083199993720065E-4
19	0.0	0.0	0.0	5.233279997488026E-4	1.3083199993720065E-4
20	0.0	0.0	0.0	5.233279997488026E-4	1.3083199993720065E-4
21	0.0	0.0	0.0	7.849919996232038E-4	1.3083199993720065E-4
22	0.0	0.0	0.0	0.001439151999309207	1.3083199993720065E-4
23	0.0	0.0	0.0	0.0034016319983672166	1.3083199993720065E-4
24	0.0	0.0	0.0	0.006279935996985631	2.616639998744013E-4
25	0.0	0.0	0.0	0.008111583996106439	2.616639998744013E-4
26	0.0	0.0	0.0	0.008896575995729643	2.616639998744013E-4
27	0.0	0.0	0.0	0.01020489599510165	2.616639998744013E-4
28	0.0	0.0	0.0	0.015569007992526876	2.616639998744013E-4
29	0.0	0.0	0.0	0.025250575987879723	2.616639998744013E-4
30	0.0	0.0	0.0	0.04343622397915061	2.616639998744013E-4
31	0.0	0.0	0.0	0.07679838396313678	2.616639998744013E-4
32	0.0	0.0	0.0	0.11330051194561576	2.616639998744013E-4
33	0.0	0.0	0.0	0.14875598392859712	2.616639998744013E-4
34	0.0	0.0	0.0	0.1838189599117669	3.924959998116019E-4
35	0.0	0.0	0.0	0.22607769589148272	3.924959998116019E-4
36	0.0	0.0	0.0	0.2781488318664886	3.924959998116019E-4
37	0.0	0.0	0.0	0.3590030078276786	3.924959998116019E-4
38	0.0	0.0	0.0	0.46157529577844386	5.233279997488026E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	55	4.950236E-6	28.005579	35
TAACGGC	60	9.731788E-6	25.671783	36
CGTCTTA	60	2.8713804E-4	22.000067	15
GTATAGA	50	0.0025824748	21.995749	1
GTACTAC	70	3.2181575E-5	21.995747	1
GTATATA	120	5.984475E-10	21.995747	1
TCACGTC	105	7.736526E-8	20.952444	42
GGCCGCG	85	6.1084284E-6	20.705944	40
ACTTCGC	65	4.936311E-4	20.303766	8
TAGGACG	290	0.0	19.720325	4
TCTAGAC	80	8.996405E-5	19.246279	3
TAGGACC	540	0.0	19.144447	4
ATGCGGC	305	0.0	18.757837	35
TAAGGCC	130	3.5901394E-8	18.611786	4
CGCCTCG	95	1.596716E-5	18.525158	11
TGCGGCG	310	0.0	18.45529	36
GTCCTAA	180	1.4551915E-11	18.32979	1
GTAATGG	60	0.007417936	18.32979	1
TTAGGAC	265	0.0	18.26062	3
CGGCGTT	315	0.0	18.158783	41
>>END_MODULE