FastQCFastQC Report
Thu 2 Feb 2017
SRR4064193_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064193_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322921
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT127280.9621133839435612No Hit
GTCCTACAGTGGACATTTCTAAATT112960.8538680692195528No Hit
CTGTAGGACGTGGAATATGGCAAGA112340.8491814703977033No Hit
CTTTAGGACGTGAAATATGGCGAGG105000.7936981875712911No Hit
GTCCTACAGTGTGCATTTCTCATTT75350.5695729374618741No Hit
CTGTAGGACCTGGAATATGGCGAGA57340.4334348007175031No Hit
CTGAAGGACCTGGAATATGGCGAGA55220.41740965635892086No Hit
ATTTAGAAATGTCCACTGTAGGACG52690.39828530955363173No Hit
GTCCTTCAGTGTGCATTTCTCATTT44790.3385689697268393No Hit
TTTCTAAATTTTCCACCTTTTTCAG42110.3183107683678768No Hit
GAATATGGCAAGAAAACTGAAAATC37090.2803644359716113No Hit
GGAATATGGCGAGAAAACTGAAAAT30400.22979452287778335No Hit
GTATCAACGCAGAGTACTTTTTTTT28460.215130003983609No Hit
ATTCCAGGTCCTTCAGTGTGCATTT26550.20069225600016935No Hit
GAAATATGGCGAGGAAAACTGAAAA25620.19366235776739502No Hit
CTGTAGGACATGGAATATGGCAAGA25290.19116787774931382No Hit
GCCATATTCCACGTCCTACAGTGGA24880.1880686753026069No Hit
TTCCAGGTCCTTCAGTGTGCATTTC24250.18330648617717915No Hit
ACAGTGGACATTTCTAAATTTTCCA23230.1755962752122009No Hit
CCATATTCCAGGTCCTTCAGTGTGC22800.17234589215833748No Hit
ACCTGGAATATGGCGAGAAAACTGA22000.16629866787208006No Hit
GACCTGGAATATGGCGAGAAAACTG21780.16463568119335925No Hit
GTCCACTGTAGGACGTGGAATATGG21750.16440891028262458No Hit
CCATATTTCACGTCCTAAAGTGTGT21550.1628971042110602No Hit
ATACACACTTTAGGACGTGAAATAT21460.16221679147885626No Hit
CCTAAAGTGTGTATTTCTCATTTTC20820.1573790120498503No Hit
GATATACACTGTTCTACAAATCCCG20640.15601838658544237No Hit
TATCAACGCAGAGTACTTTTTTTTT19930.15065147503138887No Hit
CACTTTAGGACGTGAAATATGGCGA19600.14815699501330767No Hit
GTGTATTTCTCATTTTCCGTGATTT18740.1416562289055809No Hit
TCCTAAAGTGTGTATTTCTCATTTT18590.14052237435190762No Hit
TTGTAGAACAGTGTATATCAATGAG18580.14044678404832941No Hit
CCACTGTAGGACGTGGAATATGGCA18350.1387082070660304No Hit
GAAATACACACTTTAGGACGTGAAA17760.13424837915491553No Hit
ATTCCACGTCCTACAGTGGACATTT17000.12850351608297095No Hit
CTACAGTGGACATTTCTAAATTTTC16130.12192715967166597No Hit
AGTGTGTATTTCTCATTTTCCGTGA15840.11973504086789763No Hit
GTAGGACGTGGAATATGGCAAGAAA15340.11595552568898673No Hit
GTGTATATCAATGAGTTACAATGAA15010.11346104567090552No Hit
ATATTTCACGTCCTAAAGTGTGTAT14370.10862326624189954No Hit
TTTCTAAATATTCCACCTTTTTCAG13550.10242486134848566No Hit
TTTTTCAAGTCGTCAAGTGGATGTT13380.10113982618765595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCGC307.7392836E-418.9936471
CCCCGAT350.002167689616.28827714
TCCCCGA350.002168785816.28704513
CCAACGT1250.015.96371819
AGCCCCG508.69788E-515.20296616
AGGCCCG508.709116E-515.20066510
CATCGCC704.424728E-714.93148515
CCAACGA1850.014.89536219
TTTGGCG400.00527172214.2527816
ATCCCCG400.00527701814.25062412
ATCGAGG400.00527701814.25062410
CGCCCCT400.00528099314.2490065
CCGCCCC400.005284970614.2473894
TCCAACG3350.014.18240818
CCGCCTG551.9559328E-413.82140219
GCATCGC551.9571888E-413.82035614
CGCATCG551.9584456E-413.8193113
ATCGCCA707.245957E-613.57407816
TTGGCGA852.6915768E-713.41438317
GCCGCCT500.001496710513.30309918
Produced by FastQC (version 0.11.5)