##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064193_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1322921
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244683544973586	32.0	32.0	32.0	32.0	32.0
2	30.884727054752325	32.0	32.0	32.0	32.0	32.0
3	30.863739406963834	32.0	32.0	32.0	32.0	32.0
4	30.900888261657347	32.0	32.0	32.0	32.0	32.0
5	30.820522918602094	32.0	32.0	32.0	32.0	32.0
6	34.374141010687715	36.0	36.0	36.0	32.0	36.0
7	34.26041615485732	36.0	36.0	36.0	32.0	36.0
8	34.071726883162334	36.0	36.0	36.0	32.0	36.0
9	34.443987963000055	36.0	36.0	36.0	32.0	36.0
10	34.01905102421082	36.0	36.0	36.0	32.0	36.0
11	34.384974612996544	36.0	36.0	36.0	32.0	36.0
12	34.1116589728336	36.0	36.0	36.0	32.0	36.0
13	34.20930879470505	36.0	36.0	36.0	32.0	36.0
14	34.11539237792733	36.0	36.0	36.0	32.0	36.0
15	34.018097830482695	36.0	36.0	36.0	32.0	36.0
16	34.23083464545502	36.0	36.0	36.0	32.0	36.0
17	33.961745259165134	36.0	36.0	36.0	32.0	36.0
18	34.124386112247066	36.0	36.0	36.0	32.0	36.0
19	34.05766481898768	36.0	36.0	36.0	32.0	36.0
20	33.91088659111164	36.0	36.0	36.0	32.0	36.0
21	33.90784559319869	36.0	36.0	36.0	32.0	36.0
22	33.88020070737406	36.0	36.0	36.0	32.0	36.0
23	33.77602366278863	36.0	36.0	36.0	32.0	36.0
24	33.78622079474133	36.0	36.0	36.0	32.0	36.0
25	33.04164950136856	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	12.0
5	19.0
6	44.0
7	16.0
8	50.0
9	85.0
10	110.0
11	48.0
12	78.0
13	68.0
14	164.0
15	416.0
16	707.0
17	940.0
18	1100.0
19	1391.0
20	1833.0
21	2319.0
22	3048.0
23	4280.0
24	6025.0
25	8577.0
26	12059.0
27	17100.0
28	24684.0
29	34822.0
30	49162.0
31	72829.0
32	113314.0
33	172514.0
34	353656.0
35	441448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.86871267797554	21.56024872149499	13.374111398374069	25.196927202155408
2	10.89855865648975	21.002023641148025	49.60128146300864	18.49813623935358
3	17.86161354111863	27.700876254059253	31.004744108416034	23.432766096406088
4	10.013493440373734	18.162548710563815	41.49040151489381	30.333556334168644
5	9.4410098471894	41.71259149056481	37.51676350567805	11.329635156567742
6	27.837274317604173	40.700395988406115	19.968006435000596	11.494323258989118
7	25.59026572825964	32.88786973591993	23.950850818838	17.57101371698243
8	21.384255573022646	44.85873963222323	20.563302323576714	13.19370247117741
9	26.45544338814319	15.089436895054106	22.30526593608701	36.14985378071569
10	15.123580039630319	29.879067023642058	36.10238840737547	18.89496452935215
11	31.856501631694577	20.5957292332359	31.20639518394851	16.34137395112101
12	26.008006722439923	23.917606242611406	35.28237197450495	14.79201506044372
13	29.9993873462031	24.451391215754732	26.9685663220426	18.580655115999566
14	17.545659043841454	27.196648921927686	28.33137430204783	26.926317732183026
15	18.148938374839563	42.22676189388513	22.581647910898063	17.042651820377245
16	16.527997966864735	28.67953613050792	36.66382774019436	18.128638162432985
17	16.999674014081684	33.751568476329716	31.98693333474518	17.26182417484342
18	17.358198263348083	27.356617288302527	39.88777346518715	15.397410983162239
19	23.722444150780976	25.75444573626113	28.820035143801388	21.70307496915651
20	23.93471120518147	29.413317679595625	30.99228256610351	15.659688549119402
21	20.041967637716713	27.43153972363993	27.603706889182895	24.922785749460466
22	22.504841296257073	34.27494024024932	27.80379134013132	15.416427123362281
23	16.696585881005497	33.86091007365952	31.98057634014251	17.46192770519248
24	22.888025525695898	27.812221531329946	33.02871035627348	16.271042586700677
25	18.999526458560588	31.404194094499505	32.72262121072291	16.873658236216993
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	93.0
1	93.0
2	339.0
3	585.0
4	585.0
5	585.0
6	1351.0
7	2117.0
8	2117.0
9	2117.0
10	2603.0
11	3089.0
12	3089.0
13	3089.0
14	4557.5
15	6026.0
16	6026.0
17	6026.0
18	11839.5
19	17653.0
20	17653.0
21	17653.0
22	33389.0
23	49125.0
24	49125.0
25	49125.0
26	79451.0
27	109777.0
28	109777.0
29	109777.0
30	148131.0
31	186485.0
32	186485.0
33	186485.0
34	195803.5
35	205122.0
36	205122.0
37	205122.0
38	201030.5
39	196939.0
40	196939.0
41	196939.0
42	188424.5
43	179910.0
44	179910.0
45	179910.0
46	170670.5
47	161431.0
48	161431.0
49	161431.0
50	133415.0
51	105399.0
52	105399.0
53	105399.0
54	73846.5
55	42294.0
56	42294.0
57	42294.0
58	32548.0
59	22802.0
60	22802.0
61	22802.0
62	18517.0
63	14232.0
64	14232.0
65	14232.0
66	11471.0
67	8710.0
68	8710.0
69	8710.0
70	6531.0
71	4352.0
72	4352.0
73	4352.0
74	3473.0
75	2594.0
76	2594.0
77	2594.0
78	2357.0
79	2120.0
80	2120.0
81	2120.0
82	1470.0
83	820.0
84	820.0
85	820.0
86	659.5
87	499.0
88	499.0
89	499.0
90	347.0
91	195.0
92	195.0
93	195.0
94	128.0
95	61.0
96	61.0
97	61.0
98	276.0
99	491.0
100	491.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036132165110388303
2	0.0043842376075366556
3	0.0012850351608297095
4	0.004233057000380219
5	0.008088162482869348
6	0.011716497054623822
7	0.019199937108867422
8	0.032806191752946696
9	0.04474945971830518
10	0.05298880280833096
11	0.0510990452188755
12	0.05911161739816664
13	0.06077460407688743
14	0.06342026470212507
15	0.056692727683663645
16	0.06236200045203001
17	0.05880925618385376
18	0.07196196900646373
19	0.06984544050627362
20	0.07400290720307562
21	0.0718107883993073
22	0.07264228173866769
23	0.07770683207840831
24	0.07249110113151125
25	0.073095823560137
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1322921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.19709093133868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.29642967745711	39.31349165742801
2	10.35192327777482	9.771613285107593
3	2.420532194897014	3.427262343143616
4	0.9721348681175965	1.8352775107228454
5	0.5614146568763403	1.3248569305389468
6	0.3742737530472964	1.0598779413471986
7	0.26511122543351756	0.8758735029593049
8	0.20387715958924754	0.7697927071965412
9	0.16636780808466786	0.7066868909597614
>10	1.1301681275263606	10.480160159585807
>50	0.13806160133817535	4.5664959636593565
>100	0.0986567394031426	9.426147590037864
>500	0.01156886681481996	3.908798189592496
>1k	0.008194613993830804	7.238970179961222
>5k	6.427148230455533E-4	1.8246063372639436
>10k+	6.427148230455533E-4	3.470088810495575
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	12728	0.9621133839435612	No Hit
GTCCTACAGTGGACATTTCTAAATT	11296	0.8538680692195528	No Hit
CTGTAGGACGTGGAATATGGCAAGA	11234	0.8491814703977033	No Hit
CTTTAGGACGTGAAATATGGCGAGG	10500	0.7936981875712911	No Hit
GTCCTACAGTGTGCATTTCTCATTT	7535	0.5695729374618741	No Hit
CTGTAGGACCTGGAATATGGCGAGA	5734	0.4334348007175031	No Hit
CTGAAGGACCTGGAATATGGCGAGA	5522	0.41740965635892086	No Hit
ATTTAGAAATGTCCACTGTAGGACG	5269	0.39828530955363173	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	4479	0.3385689697268393	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	4211	0.3183107683678768	No Hit
GAATATGGCAAGAAAACTGAAAATC	3709	0.2803644359716113	No Hit
GGAATATGGCGAGAAAACTGAAAAT	3040	0.22979452287778335	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2846	0.215130003983609	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	2655	0.20069225600016935	No Hit
GAAATATGGCGAGGAAAACTGAAAA	2562	0.19366235776739502	No Hit
CTGTAGGACATGGAATATGGCAAGA	2529	0.19116787774931382	No Hit
GCCATATTCCACGTCCTACAGTGGA	2488	0.1880686753026069	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	2425	0.18330648617717915	No Hit
ACAGTGGACATTTCTAAATTTTCCA	2323	0.1755962752122009	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	2280	0.17234589215833748	No Hit
ACCTGGAATATGGCGAGAAAACTGA	2200	0.16629866787208006	No Hit
GACCTGGAATATGGCGAGAAAACTG	2178	0.16463568119335925	No Hit
GTCCACTGTAGGACGTGGAATATGG	2175	0.16440891028262458	No Hit
CCATATTTCACGTCCTAAAGTGTGT	2155	0.1628971042110602	No Hit
ATACACACTTTAGGACGTGAAATAT	2146	0.16221679147885626	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	2082	0.1573790120498503	No Hit
GATATACACTGTTCTACAAATCCCG	2064	0.15601838658544237	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1993	0.15065147503138887	No Hit
CACTTTAGGACGTGAAATATGGCGA	1960	0.14815699501330767	No Hit
GTGTATTTCTCATTTTCCGTGATTT	1874	0.1416562289055809	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	1859	0.14052237435190762	No Hit
TTGTAGAACAGTGTATATCAATGAG	1858	0.14044678404832941	No Hit
CCACTGTAGGACGTGGAATATGGCA	1835	0.1387082070660304	No Hit
GAAATACACACTTTAGGACGTGAAA	1776	0.13424837915491553	No Hit
ATTCCACGTCCTACAGTGGACATTT	1700	0.12850351608297095	No Hit
CTACAGTGGACATTTCTAAATTTTC	1613	0.12192715967166597	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	1584	0.11973504086789763	No Hit
GTAGGACGTGGAATATGGCAAGAAA	1534	0.11595552568898673	No Hit
GTGTATATCAATGAGTTACAATGAA	1501	0.11346104567090552	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	1437	0.10862326624189954	No Hit
TTTCTAAATATTCCACCTTTTTCAG	1355	0.10242486134848566	No Hit
TTTTTCAAGTCGTCAAGTGGATGTT	1338	0.10113982618765595	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.55903035782182E-5	0.0
8	0.0	0.0	0.0	7.55903035782182E-5	0.0
9	0.0	0.0	0.0	1.511806071564364E-4	0.0
10	0.0	0.0	0.0	1.511806071564364E-4	0.0
11	0.0	0.0	0.0	1.511806071564364E-4	0.0
12	0.0	0.0	0.0	1.511806071564364E-4	0.0
13	0.0	0.0	0.0	1.511806071564364E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCCGC	30	7.7392836E-4	18.993647	1
CCCCGAT	35	0.0021676896	16.288277	14
TCCCCGA	35	0.0021687858	16.287045	13
CCAACGT	125	0.0	15.963718	19
AGCCCCG	50	8.69788E-5	15.202966	16
AGGCCCG	50	8.709116E-5	15.200665	10
CATCGCC	70	4.424728E-7	14.931485	15
CCAACGA	185	0.0	14.895362	19
TTTGGCG	40	0.005271722	14.25278	16
ATCCCCG	40	0.005277018	14.250624	12
ATCGAGG	40	0.005277018	14.250624	10
CGCCCCT	40	0.005280993	14.249006	5
CCGCCCC	40	0.0052849706	14.247389	4
TCCAACG	335	0.0	14.182408	18
CCGCCTG	55	1.9559328E-4	13.821402	19
GCATCGC	55	1.9571888E-4	13.820356	14
CGCATCG	55	1.9584456E-4	13.81931	13
ATCGCCA	70	7.245957E-6	13.574078	16
TTGGCGA	85	2.6915768E-7	13.414383	17
GCCGCCT	50	0.0014967105	13.303099	18
>>END_MODULE