FastQCFastQC Report
Thu 2 Feb 2017
SRR4064193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322921
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA58260.4403891086466992No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA49280.37250901603345926No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC46530.3517216825494493No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA44870.33917369215546506No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA29340.22178195069849221No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA28520.21558354580507832No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT19170.1449066119594443No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA17920.13545782401216702No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA17040.12880587729728382No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA16600.1254799039398422No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG15970.12071771481441446No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT14980.11323427476017085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT250.002353001435.19960820
AGGACGC300.005747643829.3307885
CTACGTC300.005747643829.3307884
GGACGCG300.005747643829.3307886
GGCCGCG554.9619466E-627.99968740
TAGGACC25250.026.0492254
CGGCCGC609.754731E-625.6663839
TCGGAAA1300.025.38529238
CTCGGAA1151.2732926E-1124.87022837
GTATCAA28850.024.5543171
CGTCGAA450.001398066324.4450979
GTGTAGG2350.024.3401531
ATCGTGG551.5930331E-424.00154535
GAGCGAT1102.0190782E-1024.00063925
TCTCGGA1202.3646862E-1123.8348736
CCGTTCT1301.8189894E-1223.692949
TAACGGC703.2140495E-522.0014236
CTGTACG602.8731933E-421.9997541
GACGGGA803.617508E-621.9989227
GCCGTTC1205.984475E-1021.9989228
Produced by FastQC (version 0.11.5)