##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064193_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1322921
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23747147410919	32.0	32.0	32.0	32.0	32.0
2	31.20569179867883	32.0	32.0	32.0	32.0	32.0
3	31.372194560370573	32.0	32.0	32.0	32.0	32.0
4	31.481959240196506	32.0	32.0	32.0	32.0	32.0
5	31.41246529460187	32.0	32.0	32.0	32.0	32.0
6	35.00476823634971	36.0	36.0	36.0	36.0	36.0
7	35.033654314959094	36.0	36.0	36.0	36.0	36.0
8	34.9277500319369	36.0	36.0	36.0	36.0	36.0
9	35.11792162948505	36.0	36.0	36.0	36.0	36.0
10	34.937433149825274	36.0	36.0	36.0	36.0	36.0
11	35.116997160072295	36.0	36.0	36.0	36.0	36.0
12	35.04263822253937	36.0	36.0	36.0	36.0	36.0
13	35.08060647612367	36.0	36.0	36.0	36.0	36.0
14	35.00719014967636	36.0	36.0	36.0	36.0	36.0
15	34.95243555737644	36.0	36.0	36.0	36.0	36.0
16	35.01426011077003	36.0	36.0	36.0	36.0	36.0
17	35.02885357477884	36.0	36.0	36.0	36.0	36.0
18	35.02661307818078	36.0	36.0	36.0	36.0	36.0
19	35.00272200683185	36.0	36.0	36.0	36.0	36.0
20	34.98337769224315	36.0	36.0	36.0	36.0	36.0
21	34.95779944531835	36.0	36.0	36.0	36.0	36.0
22	34.930351850186064	36.0	36.0	36.0	36.0	36.0
23	34.915057664063085	36.0	36.0	36.0	36.0	36.0
24	34.918755541714134	36.0	36.0	36.0	36.0	36.0
25	34.8669814750843	36.0	36.0	36.0	32.0	36.0
26	34.81774195133345	36.0	36.0	36.0	32.0	36.0
27	34.75363079125662	36.0	36.0	36.0	32.0	36.0
28	34.703707931161425	36.0	36.0	36.0	32.0	36.0
29	34.687991951144475	36.0	36.0	36.0	32.0	36.0
30	34.617161568982574	36.0	36.0	36.0	32.0	36.0
31	34.631992386544624	36.0	36.0	36.0	32.0	36.0
32	34.5915583772576	36.0	36.0	36.0	32.0	36.0
33	34.63595180664605	36.0	36.0	36.0	32.0	36.0
34	34.614020035965865	36.0	36.0	36.0	32.0	36.0
35	34.58789073572798	36.0	36.0	36.0	32.0	36.0
36	34.52231085605263	36.0	36.0	36.0	32.0	36.0
37	34.41102454341567	36.0	36.0	36.0	32.0	36.0
38	34.36406784683288	36.0	36.0	36.0	32.0	36.0
39	34.20071568899428	36.0	36.0	36.0	32.0	36.0
40	34.31051665216593	36.0	36.0	36.0	32.0	36.0
41	34.2710796789831	36.0	36.0	36.0	32.0	36.0
42	34.26650268610144	36.0	36.0	36.0	32.0	36.0
43	34.25988173141102	36.0	36.0	36.0	32.0	36.0
44	34.12449193867207	36.0	36.0	36.0	32.0	36.0
45	34.1112175254607	36.0	36.0	36.0	32.0	36.0
46	33.946110916676055	36.0	36.0	36.0	32.0	36.0
47	33.9762533061309	36.0	36.0	36.0	32.0	36.0
48	33.913978990431026	36.0	36.0	36.0	32.0	36.0
49	33.85766723787739	36.0	36.0	36.0	32.0	36.0
50	33.375603683061954	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	6.0
21	18.0
22	60.0
23	228.0
24	632.0
25	1569.0
26	3554.0
27	7143.0
28	13222.0
29	22059.0
30	35266.0
31	54324.0
32	83250.0
33	140748.0
34	283901.0
35	676937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.154477494033614	22.352053594309762	13.588851754613415	24.90461715704321
2	10.650689875948565	21.78016945859875	48.87419411165763	18.694946553795056
3	17.520827081366406	28.52711092230276	31.36727398619868	22.584788010132158
4	10.067872533582882	19.0837548122677	41.67709182936849	29.171280824780922
5	9.536548289731586	42.142954870321056	36.79683064975157	11.523666190195787
6	27.999425513258547	40.942460617422064	19.680933087412694	11.377180781906691
7	25.697491683164277	33.10625971809309	23.933816053741765	17.262432545000866
8	21.67635104439343	44.86722941128004	20.2851870973399	13.17123244698663
9	26.40272733113867	15.089594490912045	22.270684591864118	36.23699358608517
10	15.181096291144932	30.216756178590458	35.62526692947014	18.97688060079446
11	31.83863247759876	20.82328609936519	31.026110461872918	16.311970961163127
12	26.06627762827684	24.057237021135062	34.99848819278565	14.877997157802437
13	29.916305973026212	24.440137454135343	26.920419619113563	18.723136953724882
14	17.692061733089126	27.296565705737528	28.243334258054713	26.76803830311863
15	18.306230685962056	42.15472094049916	22.515179095180788	17.023869278358
16	16.74103548915989	29.015708539727918	35.92335387641346	18.319902094698737
17	17.25741748751437	33.950931310335235	31.46423709352259	17.3274141086278
18	17.742925476749846	27.523516232323143	39.26205443127152	15.471503859655488
19	23.969987625867304	25.833439789677538	28.92228636479427	21.274286219660887
20	24.284118242002126	29.58884704523499	30.520502466524658	15.606532246238228
21	20.46085896285568	27.45265968262655	27.516155537632255	24.570325816885514
22	22.868276237414207	34.017022949233514	27.823677924591056	15.291022888761225
23	16.94693095112471	33.99296101496843	31.598859490913267	17.461248542993594
24	23.15829337743789	27.972881140691364	32.55827841051253	16.31054707135822
25	19.408191882197755	31.46598936423526	32.193409151897924	16.932409601669065
26	15.481334438007233	33.392472695605896	33.48628430435782	17.639908562029053
27	17.694301066490077	32.695616414331546	31.045913199137615	18.56416932004076
28	16.43856430445599	33.218280542849605	29.091271938080133	21.251883214614274
29	19.9949201972052	29.73612238696305	28.071277494810587	22.19767992102116
30	16.812662809385078	30.107660186292488	31.703740212538534	21.3759367917839
31	22.30736840832069	27.79275836600966	30.37587039233518	19.524002833334468
32	18.34606200018596	32.39441304379049	30.71117504631985	18.548349909703695
33	16.102313753711623	33.314636639070144	34.06709039120943	16.515959216008806
34	18.632632790022967	32.057151150748595	33.12474770916139	16.18546835006705
35	17.287308429046366	33.311839791726825	32.12291685249887	17.277934926727937
36	16.029361182611588	31.42772908185828	34.275930104661576	18.26697963086855
37	17.218385851919887	31.37062360860708	28.659160231769683	22.751830307703354
38	18.77408683583078	34.866928027891966	28.533781669133624	17.825203467143627
39	22.199090767525856	28.845075192346357	29.787417395761047	19.168416644366733
40	23.411989882695508	28.7603883037187	31.58490326423672	16.24271854934907
41	22.476180685113345	27.084019968613838	33.13417130554942	17.305628040723395
42	19.462393227001286	33.21248771638068	31.843072038702854	15.482047017915187
43	16.231777792056118	33.81142258892362	32.78602771929957	17.17077189972069
44	15.974298888804897	32.92569355204475	31.175977020182934	19.92403053896742
45	16.60114158029727	33.63716009412633	30.438286102609187	19.323412222967217
46	18.38475296052781	28.651468811843017	31.396788585130007	21.566989642499166
47	22.683023808515916	28.91174460725322	29.993468834852234	18.41176274937863
48	17.25159460517742	31.030510288775098	29.958621148418096	21.759273957629386
49	21.055213592387126	29.227079249869796	29.79559564205464	19.92211151568844
50	17.537264636938612	34.801795747685205	28.54948548759811	19.111454127778078
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	9.5
2	10.0
3	34.0
4	58.0
5	72.0
6	86.0
7	106.0
8	126.0
9	187.5
10	249.0
11	398.0
12	547.0
13	986.5
14	1426.0
15	2110.5
16	2795.0
17	3219.0
18	3643.0
19	4183.0
20	4723.0
21	5715.5
22	6708.0
23	8726.5
24	10745.0
25	14481.0
26	18217.0
27	26958.5
28	35700.0
29	48523.0
30	61346.0
31	79485.5
32	97625.0
33	124383.0
34	151141.0
35	176640.5
36	202140.0
37	195289.0
38	188438.0
39	165865.5
40	143293.0
41	120665.5
42	98038.0
43	83125.0
44	68212.0
45	59577.0
46	50942.0
47	46131.5
48	41321.0
49	36879.0
50	32437.0
51	27736.0
52	23035.0
53	19991.0
54	16947.0
55	15908.5
56	14870.0
57	13951.0
58	13032.0
59	11131.0
60	9230.0
61	7944.5
62	6659.0
63	5834.0
64	5009.0
65	4222.0
66	3435.0
67	3091.5
68	2748.0
69	2838.0
70	2928.0
71	2271.0
72	1614.0
73	1409.5
74	1205.0
75	942.5
76	680.0
77	586.5
78	493.0
79	452.0
80	411.0
81	324.0
82	237.0
83	209.0
84	181.0
85	139.5
86	98.0
87	78.0
88	58.0
89	43.5
90	29.0
91	24.5
92	20.0
93	14.5
94	9.0
95	10.5
96	12.0
97	6.5
98	1.0
99	3.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00710548853635251
2	1.511806071564364E-4
3	3.023612143128728E-4
4	0.0
5	0.0
6	7.55903035782182E-5
7	1.511806071564364E-4
8	0.0
9	0.0012094448572514912
10	4.5354182146930926E-4
11	2.2677091073465463E-4
12	7.55903035782182E-5
13	5.291321250475274E-4
14	0.0
15	0.003703924875332692
16	7.55903035782182E-4
17	0.0
18	3.77951517891091E-4
19	0.0
20	5.291321250475274E-4
21	0.0
22	7.55903035782182E-5
23	2.2677091073465463E-4
24	3.023612143128728E-4
25	0.001965347893033673
26	0.004308647303958437
27	0.005820453375522801
28	0.00377951517891091
29	0.002645660625237637
30	0.004157466696802001
31	0.007407849750665384
32	0.004081876393223783
33	0.003703924875332692
34	0.003250383053863383
35	0.003250383053863383
36	0.006500766107726766
37	0.004233057000380219
38	0.007785801268556474
39	0.003552744268176255
40	0.00294802183955051
41	0.003401563661019819
42	0.001587396375142582
43	0.0012094448572514912
44	0.001587396375142582
45	0.0013606254644079276
46	0.001133854553673273
47	0.0024944800180812005
48	0.0017385769822990185
49	3.023612143128728E-4
50	0.0013606254644079276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1322921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.68163179452104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.91044624144514	53.223253268147374
2	9.786159624174143	12.26824908489784
3	2.3167500991497154	4.356530300244678
4	0.8831334138135676	2.214249738804017
5	0.4652427617624195	1.4581087743929029
6	0.29046086843597585	1.0923936723612395
7	0.21682765132877094	0.9513777702422569
8	0.15337926108895197	0.7691249894794716
9	0.11573512390526369	0.6529019780090708
>10	0.7341760377586551	8.875006817453428
>50	0.07375625843612485	3.1485732519891765
>100	0.04761624508385106	5.690266517994681
>500	0.0034011457940105808	1.5383135706908544
>1k	0.002793798330794406	3.3180641010263683
>5k	1.2146949264323504E-4	0.4435861642667253
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	5826	0.4403891086466992	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	4928	0.37250901603345926	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	4653	0.3517216825494493	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	4487	0.33917369215546506	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2934	0.22178195069849221	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2852	0.21558354580507832	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	1917	0.1449066119594443	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	1792	0.13545782401216702	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	1704	0.12880587729728382	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1660	0.1254799039398422	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	1597	0.12071771481441446	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	1498	0.11323427476017085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	7.55903035782182E-5	0.0	0.0	0.0	0.0
11	7.55903035782182E-5	0.0	0.0	0.0	0.0
12	7.55903035782182E-5	0.0	0.0	0.0	0.0
13	7.55903035782182E-5	0.0	0.0	0.0	0.0
14	7.55903035782182E-5	0.0	0.0	0.0	0.0
15	7.55903035782182E-5	0.0	0.0	7.55903035782182E-5	0.0
16	7.55903035782182E-5	0.0	0.0	7.55903035782182E-5	0.0
17	7.55903035782182E-5	0.0	0.0	7.55903035782182E-5	0.0
18	7.55903035782182E-5	0.0	0.0	7.55903035782182E-5	0.0
19	7.55903035782182E-5	0.0	0.0	7.55903035782182E-5	0.0
20	7.55903035782182E-5	0.0	0.0	1.511806071564364E-4	0.0
21	7.55903035782182E-5	0.0	0.0	1.511806071564364E-4	0.0
22	7.55903035782182E-5	0.0	0.0	3.023612143128728E-4	0.0
23	7.55903035782182E-5	0.0	0.0	4.535418214693092E-4	0.0
24	7.55903035782182E-5	0.0	0.0	6.047224286257456E-4	0.0
25	7.55903035782182E-5	0.0	0.0	9.070836429386184E-4	0.0
26	7.55903035782182E-5	0.0	0.0	0.0010582642500950549	0.0
27	7.55903035782182E-5	0.0	0.0	0.0012094448572514912	0.0
28	7.55903035782182E-5	0.0	0.0	0.0017385769822990185	0.0
29	7.55903035782182E-5	0.0	0.0	0.0027968412323940736	0.0
30	7.55903035782182E-5	0.0	0.0	0.005366911554053493	0.0
31	7.55903035782182E-5	0.0	0.0	0.012699171001140657	0.0
32	7.55903035782182E-5	0.0	0.0	0.02403771653787339	0.0
33	7.55903035782182E-5	0.0	0.0	0.034620359038823934	0.0
34	7.55903035782182E-5	0.0	0.0	0.048226613682903215	0.0
35	7.55903035782182E-5	0.0	0.0	0.06462970955937657	0.0
36	7.55903035782182E-5	0.0	0.0	0.08579499456127766	0.0
37	7.55903035782182E-5	0.0	0.0	0.12699171001140658	0.0
38	7.55903035782182E-5	0.0	0.0	0.17801516492670386	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCGAT	25	0.0023530014	35.199608	20
AGGACGC	30	0.0057476438	29.330788	5
CTACGTC	30	0.0057476438	29.330788	4
GGACGCG	30	0.0057476438	29.330788	6
GGCCGCG	55	4.9619466E-6	27.999687	40
TAGGACC	2525	0.0	26.049225	4
CGGCCGC	60	9.754731E-6	25.66638	39
TCGGAAA	130	0.0	25.385292	38
CTCGGAA	115	1.2732926E-11	24.870228	37
GTATCAA	2885	0.0	24.554317	1
CGTCGAA	45	0.0013980663	24.445097	9
GTGTAGG	235	0.0	24.340153	1
ATCGTGG	55	1.5930331E-4	24.001545	35
GAGCGAT	110	2.0190782E-10	24.000639	25
TCTCGGA	120	2.3646862E-11	23.83487	36
CCGTTCT	130	1.8189894E-12	23.69294	9
TAACGGC	70	3.2140495E-5	22.00142	36
CTGTACG	60	2.8731933E-4	21.999754	1
GACGGGA	80	3.617508E-6	21.998922	7
GCCGTTC	120	5.984475E-10	21.998922	8
>>END_MODULE