FastQCFastQC Report
Thu 2 Feb 2017
SRR4064192_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064192_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441713
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45240.3137933832877972No Hit
TATCAACGCAGAGTACTTTTTTTTT31210.2164785917862987No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21450.14878134552438663No Hit
GGTATCAACGCAGAGTACTTTTTTT17060.11833145709305529No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15270.10591567114952838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT651.9291474E-716.0650351
CGTTATT601.4774487E-615.8221742
CTTAGCG456.787426E-414.7704373
GTATTAT707.3173633E-613.5613921
TTATACA1450.013.1014854
GTTATTC957.4333E-812.9935433
TCGCGTA905.3933945E-712.6687279
GCGTAAC905.397942E-712.66784711
TATACTG1301.9826984E-1012.4220795
GCGCCGA1501.4551915E-1112.036962519
ACGGTAT2350.011.7253119
CGTCTTA658.011855E-411.69420915
CGCGTAA1001.9260988E-611.40145910
ACTCTAA2100.011.31097110
ATCCCCG2200.011.22909812
CAAGACG1950.011.203454
GTTTTCG2150.011.04830715
TTTTCGG2150.011.04830716
TATTCCA2700.010.906535
CGATCCC2100.010.85928517
Produced by FastQC (version 0.11.5)