##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064192_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1441713
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248423229866138	32.0	32.0	32.0	32.0	32.0
2	30.83691691758346	32.0	32.0	32.0	32.0	32.0
3	30.860666443321243	32.0	32.0	32.0	32.0	32.0
4	30.88771343533699	32.0	32.0	32.0	32.0	32.0
5	30.795876849275828	32.0	32.0	32.0	32.0	32.0
6	34.44781659040322	36.0	36.0	36.0	32.0	36.0
7	34.393855781282404	36.0	36.0	36.0	32.0	36.0
8	34.33897384569606	36.0	36.0	36.0	32.0	36.0
9	34.48405542573314	36.0	36.0	36.0	32.0	36.0
10	34.18254673433617	36.0	36.0	36.0	32.0	36.0
11	34.48271743405241	36.0	36.0	36.0	32.0	36.0
12	34.28667148038479	36.0	36.0	36.0	32.0	36.0
13	34.37019157072177	36.0	36.0	36.0	32.0	36.0
14	34.26597387968341	36.0	36.0	36.0	32.0	36.0
15	34.20179050892931	36.0	36.0	36.0	32.0	36.0
16	34.206628503731324	36.0	36.0	36.0	32.0	36.0
17	34.12518233518044	36.0	36.0	36.0	32.0	36.0
18	34.12087565278249	36.0	36.0	36.0	32.0	36.0
19	34.14130066108858	36.0	36.0	36.0	32.0	36.0
20	34.11122602071286	36.0	36.0	36.0	32.0	36.0
21	34.11280747277718	36.0	36.0	36.0	32.0	36.0
22	34.08973075778605	36.0	36.0	36.0	32.0	36.0
23	34.03731394528592	36.0	36.0	36.0	32.0	36.0
24	34.02514092610665	36.0	36.0	36.0	32.0	36.0
25	33.6254788574425	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	7.0
5	13.0
6	59.0
7	21.0
8	92.0
9	86.0
10	110.0
11	41.0
12	93.0
13	79.0
14	130.0
15	256.0
16	427.0
17	577.0
18	731.0
19	984.0
20	1425.0
21	2127.0
22	3336.0
23	5195.0
24	7726.0
25	11324.0
26	16101.0
27	21434.0
28	29008.0
29	38811.0
30	51091.0
31	70138.0
32	97797.0
33	139553.0
34	298744.0
35	644194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.58473828868493	17.15554741390377	11.473553393854488	25.786160903556816
2	16.652712467423825	19.7724644688167	37.63915758718831	25.935665476571167
3	18.69159656404679	24.07504772157237	28.848453488114	28.38490222626684
4	12.473892924748501	15.631505092397024	36.177834009290684	35.71676797356379
5	14.365001134155705	36.86533236311573	33.82343658196685	14.946229920761708
6	33.66645439044119	35.80343776491171	17.17952880227649	13.350579042370612
7	29.965019973719887	30.701112950379976	20.971642729191327	18.36222434670881
8	28.114403472070443	32.79219269852173	19.550795682809298	19.542608146598532
9	27.492670198850135	14.129635990798281	18.593302730329242	39.784391080022345
10	15.670377549540321	26.88616882400338	32.02678609227447	25.41666753418183
11	36.560477020619814	21.38993604192807	22.773681697553826	19.27590523989829
12	24.535263350767895	23.843433165650577	29.108505180397774	22.51279830318375
13	29.332243216381737	19.75022625941822	25.47514477826577	25.44238574593427
14	23.50076278868324	19.643370262763003	25.85208146222218	31.003785486331576
15	25.027778491084856	27.10222970539184	22.720411309055756	25.14958049446755
16	25.353278900157417	26.06788785025972	23.982015397129906	24.59681785245296
17	23.82693754493894	26.02981308177707	25.45320659280255	24.690042780481438
18	24.34991396047666	25.36510397975312	26.289846642098492	23.995135417671733
19	25.242263565267308	25.08858645517439	25.849406149722043	23.819743829836266
20	25.593757657330595	24.468611625745428	25.42389940948749	24.513731307436487
21	26.56916472021407	24.239738174649723	24.988217165108924	24.20287994002728
22	25.606610450442446	24.371107600296536	25.275157778413305	24.747124170847716
23	24.35015667322422	24.37014864866741	25.79374390001791	25.485950778090462
24	24.494541597628245	25.06207383888776	25.635575719671284	24.807808843812712
25	24.71108507346342	24.690816430650354	25.741870504196374	24.856227991689856
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	176.0
1	176.0
2	430.5
3	685.0
4	685.0
5	685.0
6	1861.5
7	3038.0
8	3038.0
9	3038.0
10	3202.5
11	3367.0
12	3367.0
13	3367.0
14	3881.0
15	4395.0
16	4395.0
17	4395.0
18	7514.5
19	10634.0
20	10634.0
21	10634.0
22	16480.0
23	22326.0
24	22326.0
25	22326.0
26	32434.0
27	42542.0
28	42542.0
29	42542.0
30	52978.5
31	63415.0
32	63415.0
33	63415.0
34	77830.0
35	92245.0
36	92245.0
37	92245.0
38	105891.0
39	119537.0
40	119537.0
41	119537.0
42	136989.0
43	154441.0
44	154441.0
45	154441.0
46	171728.0
47	189015.0
48	189015.0
49	189015.0
50	193357.5
51	197700.0
52	197700.0
53	197700.0
54	182964.0
55	168228.0
56	168228.0
57	168228.0
58	153282.5
59	138337.0
60	138337.0
61	138337.0
62	120286.0
63	102235.0
64	102235.0
65	102235.0
66	83220.5
67	64206.0
68	64206.0
69	64206.0
70	48228.0
71	32250.0
72	32250.0
73	32250.0
74	24387.0
75	16524.0
76	16524.0
77	16524.0
78	12916.0
79	9308.0
80	9308.0
81	9308.0
82	6382.0
83	3456.0
84	3456.0
85	3456.0
86	2623.5
87	1791.0
88	1791.0
89	1791.0
90	1281.5
91	772.0
92	772.0
93	772.0
94	557.0
95	342.0
96	342.0
97	342.0
98	545.0
99	748.0
100	748.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03912013001200655
2	0.006381297803376955
3	0.0011791528549718287
4	0.003745544362851691
5	0.007768536456284989
6	0.01352557686585333
7	0.022126456513883137
8	0.03454224245741004
9	0.04702739033358234
10	0.05659933703864778
11	0.0533393262043139
12	0.06221765358292532
13	0.061662758121762105
14	0.0656857502151954
15	0.058957642748591436
16	0.06415978769699655
17	0.060275519468854066
18	0.07602067817936024
19	0.07144279062476373
20	0.07629812590994185
21	0.07338492473883498
22	0.07525769692026082
23	0.07872579355253091
24	0.07560450658348783
25	0.0738010963347074
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1441713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.638403795790055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.88003543864156	42.00602420131147
2	13.55007409091343	14.807088392844017
3	4.215807574584736	6.910349897565333
4	1.8134416374968338	3.96334225798603
5	0.9321549559755093	2.546572944241839
6	0.5637104625941459	1.8480143927478332
7	0.36804101717351867	1.4076421596818012
8	0.27027607174546747	1.1813962515495022
9	0.20651547834502243	1.0155308486306465
>10	1.0534602422994075	10.378954233408601
>50	0.07378560707532736	2.841301345343263
>100	0.06531315311301371	7.352204222839384
>500	0.0056018600318859055	2.1269547469495182
>1k	0.0017824100101455154	1.6146241049007477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4524	0.3137933832877972	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3121	0.2164785917862987	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2145	0.14878134552438663	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1706	0.11833145709305529	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1527	0.10591567114952838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3872386529080336E-4
2	0.0	0.0	0.0	0.0	1.3872386529080336E-4
3	0.0	0.0	0.0	0.0	1.3872386529080336E-4
4	0.0	0.0	0.0	0.0	1.3872386529080336E-4
5	0.0	0.0	0.0	0.0	1.3872386529080336E-4
6	0.0	0.0	0.0	0.0	4.161715958724101E-4
7	0.0	0.0	0.0	0.0	4.161715958724101E-4
8	0.0	0.0	0.0	6.936193264540168E-5	4.161715958724101E-4
9	0.0	0.0	0.0	6.936193264540168E-5	4.855335285178118E-4
10	0.0	0.0	0.0	6.936193264540168E-5	6.242573938086152E-4
11	0.0	0.0	0.0	6.936193264540168E-5	6.242573938086152E-4
12	0.0	0.0	0.0	6.936193264540168E-5	0.0011097909223264269
13	0.0	0.0	0.0	6.936193264540168E-5	0.0011791528549718287
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	65	1.9291474E-7	16.065035	1
CGTTATT	60	1.4774487E-6	15.822174	2
CTTAGCG	45	6.787426E-4	14.770437	3
GTATTAT	70	7.3173633E-6	13.561392	1
TTATACA	145	0.0	13.101485	4
GTTATTC	95	7.4333E-8	12.993543	3
TCGCGTA	90	5.3933945E-7	12.668727	9
GCGTAAC	90	5.397942E-7	12.667847	11
TATACTG	130	1.9826984E-10	12.422079	5
GCGCCGA	150	1.4551915E-11	12.0369625	19
ACGGTAT	235	0.0	11.725311	9
CGTCTTA	65	8.011855E-4	11.694209	15
CGCGTAA	100	1.9260988E-6	11.401459	10
ACTCTAA	210	0.0	11.310971	10
ATCCCCG	220	0.0	11.229098	12
CAAGACG	195	0.0	11.20345	4
GTTTTCG	215	0.0	11.048307	15
TTTTCGG	215	0.0	11.048307	16
TATTCCA	270	0.0	10.90653	5
CGATCCC	210	0.0	10.859285	17
>>END_MODULE