##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1441713
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31462017752493	32.0	32.0	32.0	32.0	32.0
2	31.42210620282955	32.0	32.0	32.0	32.0	32.0
3	31.493591304233227	32.0	32.0	32.0	32.0	32.0
4	31.578497245984465	32.0	32.0	32.0	32.0	32.0
5	31.529945973990664	32.0	32.0	32.0	32.0	32.0
6	35.12689418767813	36.0	36.0	36.0	36.0	36.0
7	35.14244582659656	36.0	36.0	36.0	36.0	36.0
8	35.09167081104214	36.0	36.0	36.0	36.0	36.0
9	35.19632756311416	36.0	36.0	36.0	36.0	36.0
10	35.05835072583795	36.0	36.0	36.0	36.0	36.0
11	35.194734319521295	36.0	36.0	36.0	36.0	36.0
12	35.10053595965355	36.0	36.0	36.0	36.0	36.0
13	35.14422565378824	36.0	36.0	36.0	36.0	36.0
14	35.08646172990047	36.0	36.0	36.0	36.0	36.0
15	35.06374708419776	36.0	36.0	36.0	36.0	36.0
16	35.07858568244859	36.0	36.0	36.0	36.0	36.0
17	35.04695109220767	36.0	36.0	36.0	36.0	36.0
18	35.042278178805354	36.0	36.0	36.0	36.0	36.0
19	35.04047754303388	36.0	36.0	36.0	36.0	36.0
20	35.03399220233153	36.0	36.0	36.0	36.0	36.0
21	35.026293721427216	36.0	36.0	36.0	36.0	36.0
22	35.011045194154455	36.0	36.0	36.0	36.0	36.0
23	34.96509638187351	36.0	36.0	36.0	36.0	36.0
24	34.944988357599605	36.0	36.0	36.0	36.0	36.0
25	34.915184228761206	36.0	36.0	36.0	32.0	36.0
26	34.8619212006828	36.0	36.0	36.0	32.0	36.0
27	34.85585966138892	36.0	36.0	36.0	32.0	36.0
28	34.83414798923226	36.0	36.0	36.0	32.0	36.0
29	34.8065370847041	36.0	36.0	36.0	32.0	36.0
30	34.79782591958316	36.0	36.0	36.0	32.0	36.0
31	34.77985008111878	36.0	36.0	36.0	32.0	36.0
32	34.748763450145766	36.0	36.0	36.0	32.0	36.0
33	34.71927561172023	36.0	36.0	36.0	32.0	36.0
34	34.70060337945208	36.0	36.0	36.0	32.0	36.0
35	34.662042306617195	36.0	36.0	36.0	32.0	36.0
36	34.64039652829655	36.0	36.0	36.0	32.0	36.0
37	34.63101879500289	36.0	36.0	36.0	32.0	36.0
38	34.59370068800101	36.0	36.0	36.0	32.0	36.0
39	34.58982335596613	36.0	36.0	36.0	32.0	36.0
40	34.56657046166609	36.0	36.0	36.0	32.0	36.0
41	34.55283610538297	36.0	36.0	36.0	32.0	36.0
42	34.48259951876691	36.0	36.0	36.0	32.0	36.0
43	34.47016500510157	36.0	36.0	36.0	32.0	36.0
44	34.40822965458451	36.0	36.0	36.0	32.0	36.0
45	34.37186943587247	36.0	36.0	36.0	32.0	36.0
46	34.34843203883158	36.0	36.0	36.0	32.0	36.0
47	34.305018405188825	36.0	36.0	36.0	32.0	36.0
48	34.248543226009616	36.0	36.0	36.0	32.0	36.0
49	34.23616004017443	36.0	36.0	36.0	32.0	36.0
50	33.64570063528594	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	16.0
21	41.0
22	165.0
23	474.0
24	1154.0
25	2599.0
26	5299.0
27	9762.0
28	16076.0
29	24461.0
30	35369.0
31	49291.0
32	71754.0
33	114748.0
34	240184.0
35	870314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73731004520693	18.094011277801712	12.012266985203174	26.156411691788183
2	15.88844620408571	20.49943539191015	37.11243885724432	26.499679546759815
3	17.953559089493467	24.60603883046647	29.092469114651216	28.34793296538885
4	12.074039701383008	16.2366573652315	36.331988405459335	35.35731452792616
5	14.027341086610164	37.192145732194966	33.54710680974647	15.23340637144841
6	33.82372219713633	36.0934527190918	16.87034798187989	13.212477101891986
7	29.93057564161522	30.879585604069604	20.89236900825615	18.29746974605903
8	28.075421391081306	33.008442040822274	19.383746973218663	19.53238959487776
9	27.596922238167938	13.918389231579173	18.48932195877497	39.995366571477916
10	15.627997760978177	27.0481188540481	32.06795306377675	25.255930321196974
11	36.78506054949911	21.33899049255989	22.711316329949163	19.16463262799184
12	24.501495444305103	23.960194546483628	29.091871330751218	22.446438678460055
13	29.488038509825138	19.773740904897657	25.397964916661465	25.34025566861574
14	23.619937435406566	19.52362125531487	25.730764162002068	31.125677147276498
15	25.0476362897313	27.20821168478129	22.676816127043054	25.067335898444355
16	25.48061449847957	25.960119192950526	23.91093545379886	24.648330854771046
17	23.81389361128047	26.00316429136728	25.51062520765229	24.67231688969996
18	24.5073714802959	25.22860085801187	26.354836807807423	23.90919085388481
19	25.308643259788877	25.052350918664118	25.907514186249276	23.731491635297733
20	25.614152152003566	24.396772428163082	25.466826385184742	24.522249034648606
21	26.50603830304645	24.278410474206723	25.07385311778419	24.14169810496264
22	25.659493713030063	24.385591574461472	25.354439721664246	24.60047499084422
23	24.436464678553545	24.251058817980415	25.95536386853871	25.357112634927333
24	24.584209029555183	25.03388337460377	25.665147637180848	24.7167599586602
25	24.85475319140672	24.501000219188114	25.804892056789143	24.839354532616024
26	24.55928114304582	25.110951414448447	26.032344123494234	24.2974233190115
27	24.938871065637734	25.01427208878869	25.341194832220587	24.705662013352985
28	24.451203651359027	24.86697580211632	26.037991058887183	24.643829487637472
29	24.46912556063143	25.008913224728406	25.914177784498342	24.607783430141822
30	24.47666195681503	25.179116559463782	25.958981978401276	24.385239505319912
31	24.78761548743867	24.96054750011619	25.293925459573934	24.957911552871206
32	24.67745694570456	25.028786100544647	25.230704988943366	25.063051964807432
33	24.442098370480533	24.637984756376714	25.621439848174177	25.29847702496858
34	24.899214525316367	24.814590795712768	25.799416789902864	24.486777889068
35	25.374132342444767	24.723220101146655	25.66823591268176	24.23441164372682
36	24.58537532819323	25.158139712334517	25.456487734157413	24.79999722531484
37	25.34578002702425	24.967815058668155	25.288138461837264	24.39826645247033
38	24.485105857560345	24.902001724464434	25.688623875993944	24.924268541981277
39	24.882392030265237	24.996913267677265	25.248083104545195	24.8726115975123
40	25.18702152143438	25.064872277393235	25.53120354808525	24.21690265308713
41	24.286998526690216	25.487704383582166	25.739637582440146	24.485659507287473
42	25.32453344523942	25.381550061213094	25.27244162212966	24.02147487141782
43	24.745992902783673	24.70562366996557	25.553793736803698	24.994589690447057
44	24.52327113823958	25.21939377124228	25.258514254005686	24.99882083651245
45	24.615018592723974	25.375790823705184	25.268139610860285	24.741050972710557
46	24.52228172771394	25.16353991164579	25.381754973479172	24.9324233871611
47	24.683336651237834	25.138086098581304	25.58139050949834	24.597186740682528
48	25.272684771895072	25.504705264610877	24.812944315775383	24.409665647718672
49	24.56317407073698	25.643698754323868	24.952296132293178	24.840831042645974
50	24.61798412145712	25.759072313542262	24.944613766860375	24.678329798140243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	47.5
2	40.0
3	132.0
4	224.0
5	267.5
6	311.0
7	290.0
8	269.0
9	376.5
10	484.0
11	759.0
12	1034.0
13	1942.5
14	2851.0
15	3786.0
16	4721.0
17	5042.0
18	5363.0
19	5380.5
20	5398.0
21	5965.0
22	6532.0
23	7343.0
24	8154.0
25	9017.5
26	9881.0
27	11789.0
28	13697.0
29	16111.0
30	18525.0
31	21138.0
32	23751.0
33	27349.5
34	30948.0
35	35158.5
36	39369.0
37	45778.5
38	52188.0
39	57548.0
40	62908.0
41	69255.0
42	75602.0
43	78650.0
44	81698.0
45	88816.0
46	95934.0
47	101714.5
48	107495.0
49	110618.0
50	113741.0
51	111356.0
52	108971.0
53	107092.5
54	105214.0
55	103900.0
56	102586.0
57	98924.0
58	95262.0
59	86439.5
60	77617.0
61	67814.5
62	58012.0
63	50376.0
64	42740.0
65	36073.0
66	29406.0
67	24943.5
68	20481.0
69	18337.0
70	16193.0
71	12435.5
72	8678.0
73	7378.0
74	6078.0
75	4723.0
76	3368.0
77	2884.0
78	2400.0
79	1973.5
80	1547.0
81	1242.0
82	937.0
83	749.5
84	562.0
85	403.5
86	245.0
87	164.5
88	84.0
89	67.0
90	50.0
91	40.0
92	30.0
93	28.5
94	27.0
95	26.5
96	26.0
97	20.0
98	14.0
99	13.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008323431917448202
2	3.468096632270084E-4
3	6.936193264540168E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.001109790922326427
10	8.323431917448203E-4
11	0.0
12	6.936193264540169E-5
13	2.0808579793620506E-4
14	2.0808579793620506E-4
15	0.004161715958724101
16	0.0011791528549718287
17	0.0
18	5.548954611632135E-4
19	0.0
20	6.936193264540168E-4
21	0.0
22	6.936193264540169E-5
23	7.629812590994185E-4
24	2.0808579793620506E-4
25	0.0020114960467166485
26	0.006311935870731554
27	0.006103850072795348
28	0.004022992093433297
29	0.002427667642589059
30	0.003745544362851691
31	0.007352364860412579
32	0.0031212869690430763
33	0.004231077891369503
34	0.002566391507879862
35	0.003051925036397674
36	0.007491088725703382
37	0.0031212869690430763
38	0.007352364860412579
39	0.004092354026078699
40	0.0023583057099436575
41	0.004231077891369503
42	0.0019421341140712473
43	0.0014566005855534353
44	9.017051243902219E-4
45	0.0013872386529080337
46	0.001109790922326427
47	0.0027744773058160674
48	0.0020808579793620504
49	4.161715958724101E-4
50	0.001317876720262632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1441713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.9779553123689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9318069918674	45.183268231866464
2	13.29563040969282	15.417069314858864
3	3.9594022404451845	6.886741384806726
4	1.6362966584072627	3.7947653815565934
5	0.8642850288123214	2.505473938881512
6	0.5056823707000186	1.7591057934419068
7	0.3284340261316062	1.3329353303083764
8	0.23899098383507694	1.1084966864679333
9	0.18860917032108557	0.9841656643540987
>10	0.9245615794425665	9.500289280927552
>50	0.06440596592637043	2.5750090407326596
>100	0.05611459610401484	6.464827442353774
>500	0.005057481024958929	2.012483132796933
>1k	7.224972892798469E-4	0.47536937664658013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0808579793620506E-4
2	0.0	0.0	0.0	0.0	2.0808579793620506E-4
3	0.0	0.0	0.0	0.0	2.0808579793620506E-4
4	0.0	0.0	0.0	0.0	2.0808579793620506E-4
5	0.0	0.0	0.0	0.0	2.0808579793620506E-4
6	0.0	0.0	0.0	0.0	2.774477305816067E-4
7	0.0	0.0	0.0	0.0	3.468096632270084E-4
8	0.0	0.0	0.0	0.0	3.468096632270084E-4
9	0.0	0.0	0.0	6.936193264540168E-5	3.468096632270084E-4
10	0.0	0.0	0.0	6.936193264540168E-5	3.468096632270084E-4
11	0.0	0.0	0.0	6.936193264540168E-5	3.468096632270084E-4
12	0.0	0.0	0.0	6.936193264540168E-5	8.323431917448203E-4
13	0.0	0.0	0.0	6.936193264540168E-5	9.710670570356236E-4
14	0.0	0.0	0.0	6.936193264540168E-5	0.0010404289896810252
15	0.0	0.0	0.0	6.936193264540168E-5	0.0012485147876172304
16	0.0	0.0	0.0	6.936193264540168E-5	0.001317876720262632
17	0.0	0.0	0.0	6.936193264540168E-5	0.001317876720262632
18	0.0	0.0	0.0	2.0808579793620506E-4	0.001317876720262632
19	0.0	0.0	0.0	2.0808579793620506E-4	0.001317876720262632
20	0.0	0.0	0.0	2.0808579793620506E-4	0.0014566005855534353
21	0.0	0.0	0.0	4.855335285178118E-4	0.0018034102487804438
22	0.0	0.0	0.0	6.936193264540168E-4	0.0018727721814258455
23	0.0	0.0	0.0	0.0011097909223264269	0.0018727721814258455
24	0.0	0.0	0.0	0.0021502199120074523	0.0018727721814258455
25	0.0	0.0	0.0	0.0028438392384614692	0.0018727721814258455
26	0.0	0.0	0.0	0.003745544362851691	0.0019421341140712471
27	0.0	0.0	0.0	0.004785973352532716	0.0019421341140712471
28	0.0	0.0	0.0	0.007283002927767177	0.0019421341140712471
29	0.0	0.0	0.0	0.011860890482363688	0.0019421341140712471
30	0.0	0.0	0.0	0.022750713907691754	0.0019421341140712471
31	0.0	0.0	0.0	0.04557078974802891	0.0019421341140712471
32	0.0	0.0	0.0	0.06908448491482008	0.002011496046716649
33	0.0	0.0	0.0	0.09287562781219286	0.002011496046716649
34	0.0	0.0	0.0	0.12075912473564433	0.0022889437772982556
35	0.0	0.0	0.0	0.1518332705607843	0.0022889437772982556
36	0.0	0.0	0.0	0.19677980291500458	0.0022889437772982556
37	0.0	0.0	0.0	0.2592749042285115	0.0022889437772982556
38	0.0	0.0	0.0	0.34750328255346247	0.0023583057099436575
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3215	0.0	20.93849	1
GTATAGG	160	5.2750693E-11	19.24921	1
GTCTATA	130	3.5903213E-8	18.614622	1
GTATTAC	155	7.221388E-10	18.450857	1
CGCAATA	365	0.0	18.08396	36
ATACGAA	365	0.0	18.081451	40
TACGAAT	360	0.0	17.721495	41
CGAGCCG	365	0.0	17.479948	15
AACGCAG	3870	0.0	17.450874	6
AATACGA	395	0.0	17.265715	39
TAGCGGC	375	0.0	17.014406	30
CAATACG	405	0.0	16.297325	38
CGAATGC	400	0.0	15.948793	43
CTAGCGG	415	0.0	15.904067	29
CTAGACA	210	1.5097612E-10	15.713098	4
CGCTAGA	465	0.0	15.613889	37
CGTATTG	495	0.0	15.556537	27
TCTAGCG	425	0.0	15.529852	28
ATACCGT	340	0.0	15.528236	6
GGGTATA	115	8.222158E-5	15.30372	1
>>END_MODULE