FastQCFastQC Report
Thu 2 Feb 2017
SRR4064191_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064191_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8034
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT931.157580283793876No Hit
CTGTAGGACGTGGAATATGGCAAGA680.8464027881503611No Hit
GTCCTACAGTGGACATTTCTAAATT660.8215085884988798No Hit
CTTTAGGACGTGAAATATGGCGAGG650.8090614886731391No Hit
ATTTAGAAATGTCCACTGTAGGACG550.6845904904157332No Hit
GTCCTACAGTGTGCATTTCTCATTT480.597460791635549No Hit
GATATACACTGTTCTACAAATCCCG430.5352252925068459No Hit
CTGTAGGACCTGGAATATGGCGAGA390.48543689320388345No Hit
TTTCTAAATTTTCCACCTTTTTCAG380.4729897933781429No Hit
TTGTAGAACAGTGTATATCAATGAG360.4480955937266617No Hit
CTGAAGGACCTGGAATATGGCGAGA340.42320139407518054No Hit
GTCCTTCAGTGTGCATTTCTCATTT340.42320139407518054No Hit
GTGTATATCAATGAGTTACAATGAA330.4107542942494399No Hit
GAATATGGCAAGAAAACTGAAAATC310.38586009459795867No Hit
TATCAACGCAGAGTACTTTTTTTTT300.37341299477221807No Hit
GATATACACTGTTCTACAATGCCGG300.37341299477221807No Hit
GTATCAACGCAGAGTACTTTTTTTT280.34851879512073686No Hit
CTGTAGGACATGGAATATGGCAAGA240.2987303958177745No Hit
GAAATATGGCGAGGAAAACTGAAAA220.27383619616629323No Hit
GTGTATATCAATGAGTTACAATGAG210.2613890963405527No Hit
GTTCTACAGTGTGGTTTTTATCATT200.24894199651481203No Hit
GGTATCAACGCAGAGTACTTTTTTT190.23649489668907145No Hit
ATTCCAGGTCCTTCAGTGTGCATTT190.23649489668907145No Hit
CTGTAGAACATATTAGATGAGTGAG190.23649489668907145No Hit
GTAGAACATATTAGATGAGTGAGTT190.23649489668907145No Hit
GTACTTTTTTTTTTTTTTTTTTTTT180.22404779686333084No Hit
GTGTATTTCTCATTTTCCGTGATTT180.22404779686333084No Hit
ACAGTGGACATTTCTAAATTTTCCA180.22404779686333084No Hit
CCATATTCCAGGTCCTTCAGTGTGC180.22404779686333084No Hit
CACTTTAGGACGTGAAATATGGCGA180.22404779686333084No Hit
GTGCATTTCTCATTTTTCACGTTTT170.21160069703759027No Hit
GGAATATGGCGAGAAAACTGAAAAT170.21160069703759027No Hit
TATTAGATGAGTGAGTTACACTGAA160.19915359721184964No Hit
GCCATATTCCACGTCCTACAGTGGA160.19915359721184964No Hit
GTCCACTGTAGGACGTGGAATATGG150.18670649738610903No Hit
TTGATATACACTGTTCTACAAATCC150.18670649738610903No Hit
TTCCAGGTCCTTCAGTGTGCATTTC140.17425939756036843No Hit
GAAATACACACTTTAGGACGTGAAA140.17425939756036843No Hit
CCATATTTCACGTCCTAAAGTGTGT140.17425939756036843No Hit
ATACACACTTTAGGACGTGAAATAT140.17425939756036843No Hit
ATGTTCTACAGTGTGGTTTTTATCA130.16181229773462785No Hit
CCACTGTAGGACGTGGAATATGGCA130.16181229773462785No Hit
AGTGTGTATTTCTCATTTTCCGTGA130.16181229773462785No Hit
ACCTGGAATATGGCGAGAAAACTGA130.16181229773462785No Hit
GTTCTACAAATCCCGTTTCCAACGA130.16181229773462785No Hit
CCTAAAGTGTGTATTTCTCATTTTC120.14936519790888725No Hit
GTAGGACGTGGAATATGGCAAGAAA120.14936519790888725No Hit
CCTTCAGTGTGCATTTCTCATTTTT120.14936519790888725No Hit
CACTGAAGGACCTGGAATATGGCGA100.12447099825740601No Hit
GGTCCTTCAGTGTGCATTTCTCATT100.12447099825740601No Hit
TTTCTAAATATTCCACCTTTTTCAG100.12447099825740601No Hit
ATGAGAAACATCCACTTGACGACTT100.12447099825740601No Hit
TCCTAAAGTGTGTATTTCTCATTTT100.12447099825740601No Hit
CATCTAATATGTTCTACAGTGTGGT100.12447099825740601No Hit
CTGTTCTACAATGCCGGTTTCCAAC90.11202389843166542No Hit
GACCTGGAATATGGCGAGAAAACTG90.11202389843166542No Hit
CATTGATATACACTGTTCTACAAAT90.11202389843166542No Hit
GTATTTCTCATTTTCCGTGATTTTC90.11202389843166542No Hit
ATTCCACGTCCTACAGTGGACATTT90.11202389843166542No Hit
GTGTGTATTTCTCATTTTCCGTGAT90.11202389843166542No Hit
ATATTTCACGTCCTAAAGTGTGTAT90.11202389843166542No Hit
CATCTAAAATGTTCTACAGTGTGGT90.11202389843166542No Hit
ATTTAGAAATGTCCACTGTAGGACA90.11202389843166542No Hit
GTAGAACAGTGTATATCAATGAGTT90.11202389843166542No Hit
GTGGAATATGGCAAGAAAACTGAAA90.11202389843166542No Hit
GATTAGATGAGTGAGTTACACTGAA90.11202389843166542No Hit
ACGCAGAGTACTTTTTTTTTTTTTT90.11202389843166542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACATG250.005534230719.06
ATATGGC1000.00230952358.54999915
AATATGG1100.0054773677.772727514
Produced by FastQC (version 0.11.5)