##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064191_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8034
Sequences flagged as poor quality	0
Sequence length	25
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.663057007717203	32.0	32.0	32.0	14.0	32.0
2	26.648369429922827	32.0	21.0	32.0	14.0	32.0
3	27.21197411003236	32.0	21.0	32.0	14.0	32.0
4	26.84341548419218	32.0	21.0	32.0	14.0	32.0
5	26.845407020164302	32.0	21.0	32.0	14.0	32.0
6	30.382623848643266	36.0	21.0	36.0	14.0	36.0
7	30.07580283793876	36.0	21.0	36.0	14.0	36.0
8	29.3915857605178	36.0	21.0	36.0	14.0	36.0
9	30.586631814787154	36.0	27.0	36.0	14.0	36.0
10	29.058750311177494	36.0	21.0	36.0	14.0	36.0
11	31.09447348767737	36.0	32.0	36.0	14.0	36.0
12	29.934528254916604	36.0	21.0	36.0	14.0	36.0
13	30.29512073686831	36.0	21.0	36.0	14.0	36.0
14	29.407518048294747	36.0	21.0	36.0	14.0	36.0
15	28.99763505103311	36.0	21.0	36.0	14.0	36.0
16	29.506348020911126	36.0	21.0	36.0	14.0	36.0
17	29.04991287030122	36.0	21.0	36.0	14.0	36.0
18	29.14251929300473	36.0	21.0	36.0	14.0	36.0
19	29.668782673637043	36.0	21.0	36.0	14.0	36.0
20	29.23512571570824	36.0	21.0	36.0	14.0	36.0
21	29.199651481204878	36.0	21.0	36.0	14.0	36.0
22	28.790639780931045	36.0	21.0	36.0	14.0	36.0
23	28.773711725168035	36.0	21.0	36.0	14.0	36.0
24	29.0949713716704	36.0	21.0	36.0	14.0	36.0
25	27.8991784914115	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	0.0
13	0.0
14	14.0
15	25.0
16	71.0
17	126.0
18	209.0
19	296.0
20	312.0
21	314.0
22	300.0
23	281.0
24	285.0
25	330.0
26	266.0
27	307.0
28	288.0
29	333.0
30	354.0
31	394.0
32	486.0
33	551.0
34	1016.0
35	1474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.69463463214241	21.64820117017304	13.45698991659405	24.200174281090504
2	10.53024645257655	20.998257406024397	52.34005476723923	16.13144137415982
3	20.338561115260145	26.84839432412248	32.00149365197909	20.81155090863829
4	10.007468259895443	17.550410754294248	42.867811799850635	29.574309185959674
5	9.783420463032114	41.37415982076176	37.888971869554396	10.95344784665173
6	27.548860948587077	40.918710319930284	20.07967135565791	11.452757375824724
7	24.175276982447404	33.337482883107185	23.017552595543382	19.46968753890203
8	20.204157848873397	48.462591808788744	19.955184862442422	11.378065479895431
9	26.518924302788843	14.330179282868528	21.887450199203187	37.26344621513944
10	14.904744116548374	30.818079940231602	36.90698543145312	17.370190511766904
11	33.901892430278885	18.849601593625497	31.698207171314742	15.550298804780876
12	25.68190310125794	22.319093286835223	37.850292689002366	14.14871092290447
13	31.225099601593627	23.979083665338646	27.278386454183266	17.517430278884465
14	17.006972111553782	27.290836653386453	28.448705179282868	27.253486055776893
15	18.364043824701195	43.936752988047814	22.086653386454184	15.612549800796813
16	15.189243027888446	28.548306772908365	39.11852589641434	17.143924302788843
17	16.01294981944963	33.00958784709251	32.785456356618106	18.192005976839745
18	16.63760896637609	27.496886674968867	41.38231631382317	14.483188044831879
19	24.794520547945208	23.8107098381071	28.306351183063512	23.088418430884182
20	23.02615193026152	30.747198007471983	31.880448318804483	14.346201743462018
21	20.273972602739725	26.50062266500623	27.75840597758406	25.46699875466999
22	21.058530510585307	35.84059775840598	28.866749688667497	14.234122042341221
23	17.322540473225402	34.27148194271482	31.40722291407223	16.998754669987548
24	23.636363636363637	28.268991282689914	33.038605230386054	15.0560398505604
25	20.144422310756973	30.75199203187251	33.62798804780876	15.475597609561753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	13.0
7	24.0
8	24.0
9	24.0
10	19.0
11	14.0
12	14.0
13	14.0
14	21.0
15	28.0
16	28.0
17	28.0
18	54.5
19	81.0
20	81.0
21	81.0
22	193.5
23	306.0
24	306.0
25	306.0
26	525.5
27	745.0
28	745.0
29	745.0
30	1044.5
31	1344.0
32	1344.0
33	1344.0
34	1306.5
35	1269.0
36	1269.0
37	1269.0
38	1248.0
39	1227.0
40	1227.0
41	1227.0
42	1105.0
43	983.0
44	983.0
45	983.0
46	929.0
47	875.0
48	875.0
49	875.0
50	722.0
51	569.0
52	569.0
53	569.0
54	388.0
55	207.0
56	207.0
57	207.0
58	164.0
59	121.0
60	121.0
61	121.0
62	109.5
63	98.0
64	98.0
65	98.0
66	81.0
67	64.0
68	64.0
69	64.0
70	47.5
71	31.0
72	31.0
73	31.0
74	25.5
75	20.0
76	20.0
77	20.0
78	16.0
79	12.0
80	12.0
81	12.0
82	8.0
83	4.0
84	4.0
85	4.0
86	2.5
87	1.0
88	1.0
89	1.0
90	1.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	3.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012447099825740602
2	0.0
3	0.0
4	0.0
5	0.0
6	0.012447099825740602
7	0.012447099825740602
8	0.012447099825740602
9	0.024894199651481205
10	0.03734129947722181
11	0.024894199651481205
12	0.062235499128703006
13	0.024894199651481205
14	0.024894199651481205
15	0.024894199651481205
16	0.024894199651481205
17	0.03734129947722181
18	0.04978839930296241
19	0.04978839930296241
20	0.04978839930296241
21	0.04978839930296241
22	0.04978839930296241
23	0.04978839930296241
24	0.04978839930296241
25	0.024894199651481205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	8034.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.84067712223052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.27107467474603	62.34752302713468
2	5.221885581892711	7.294000497883993
3	2.1208340759222954	4.443614637789395
4	0.9089288896809837	2.539208364451083
5	0.6415968633042238	2.2404779686333085
6	0.3742648369274639	1.568334578043316
7	0.03564427018356799	0.17425939756036843
8	0.23168775619319196	1.2944983818770228
9	0.23168775619319196	1.4563106796116505
>10	0.8732846194974158	12.322628827483197
>50	0.08911067545891997	4.319143639531989
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	93	1.157580283793876	No Hit
CTGTAGGACGTGGAATATGGCAAGA	68	0.8464027881503611	No Hit
GTCCTACAGTGGACATTTCTAAATT	66	0.8215085884988798	No Hit
CTTTAGGACGTGAAATATGGCGAGG	65	0.8090614886731391	No Hit
ATTTAGAAATGTCCACTGTAGGACG	55	0.6845904904157332	No Hit
GTCCTACAGTGTGCATTTCTCATTT	48	0.597460791635549	No Hit
GATATACACTGTTCTACAAATCCCG	43	0.5352252925068459	No Hit
CTGTAGGACCTGGAATATGGCGAGA	39	0.48543689320388345	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	38	0.4729897933781429	No Hit
TTGTAGAACAGTGTATATCAATGAG	36	0.4480955937266617	No Hit
CTGAAGGACCTGGAATATGGCGAGA	34	0.42320139407518054	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	34	0.42320139407518054	No Hit
GTGTATATCAATGAGTTACAATGAA	33	0.4107542942494399	No Hit
GAATATGGCAAGAAAACTGAAAATC	31	0.38586009459795867	No Hit
TATCAACGCAGAGTACTTTTTTTTT	30	0.37341299477221807	No Hit
GATATACACTGTTCTACAATGCCGG	30	0.37341299477221807	No Hit
GTATCAACGCAGAGTACTTTTTTTT	28	0.34851879512073686	No Hit
CTGTAGGACATGGAATATGGCAAGA	24	0.2987303958177745	No Hit
GAAATATGGCGAGGAAAACTGAAAA	22	0.27383619616629323	No Hit
GTGTATATCAATGAGTTACAATGAG	21	0.2613890963405527	No Hit
GTTCTACAGTGTGGTTTTTATCATT	20	0.24894199651481203	No Hit
GGTATCAACGCAGAGTACTTTTTTT	19	0.23649489668907145	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	19	0.23649489668907145	No Hit
CTGTAGAACATATTAGATGAGTGAG	19	0.23649489668907145	No Hit
GTAGAACATATTAGATGAGTGAGTT	19	0.23649489668907145	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	18	0.22404779686333084	No Hit
GTGTATTTCTCATTTTCCGTGATTT	18	0.22404779686333084	No Hit
ACAGTGGACATTTCTAAATTTTCCA	18	0.22404779686333084	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	18	0.22404779686333084	No Hit
CACTTTAGGACGTGAAATATGGCGA	18	0.22404779686333084	No Hit
GTGCATTTCTCATTTTTCACGTTTT	17	0.21160069703759027	No Hit
GGAATATGGCGAGAAAACTGAAAAT	17	0.21160069703759027	No Hit
TATTAGATGAGTGAGTTACACTGAA	16	0.19915359721184964	No Hit
GCCATATTCCACGTCCTACAGTGGA	16	0.19915359721184964	No Hit
GTCCACTGTAGGACGTGGAATATGG	15	0.18670649738610903	No Hit
TTGATATACACTGTTCTACAAATCC	15	0.18670649738610903	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	14	0.17425939756036843	No Hit
GAAATACACACTTTAGGACGTGAAA	14	0.17425939756036843	No Hit
CCATATTTCACGTCCTAAAGTGTGT	14	0.17425939756036843	No Hit
ATACACACTTTAGGACGTGAAATAT	14	0.17425939756036843	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	13	0.16181229773462785	No Hit
CCACTGTAGGACGTGGAATATGGCA	13	0.16181229773462785	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	13	0.16181229773462785	No Hit
ACCTGGAATATGGCGAGAAAACTGA	13	0.16181229773462785	No Hit
GTTCTACAAATCCCGTTTCCAACGA	13	0.16181229773462785	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	12	0.14936519790888725	No Hit
GTAGGACGTGGAATATGGCAAGAAA	12	0.14936519790888725	No Hit
CCTTCAGTGTGCATTTCTCATTTTT	12	0.14936519790888725	No Hit
CACTGAAGGACCTGGAATATGGCGA	10	0.12447099825740601	No Hit
GGTCCTTCAGTGTGCATTTCTCATT	10	0.12447099825740601	No Hit
TTTCTAAATATTCCACCTTTTTCAG	10	0.12447099825740601	No Hit
ATGAGAAACATCCACTTGACGACTT	10	0.12447099825740601	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	10	0.12447099825740601	No Hit
CATCTAATATGTTCTACAGTGTGGT	10	0.12447099825740601	No Hit
CTGTTCTACAATGCCGGTTTCCAAC	9	0.11202389843166542	No Hit
GACCTGGAATATGGCGAGAAAACTG	9	0.11202389843166542	No Hit
CATTGATATACACTGTTCTACAAAT	9	0.11202389843166542	No Hit
GTATTTCTCATTTTCCGTGATTTTC	9	0.11202389843166542	No Hit
ATTCCACGTCCTACAGTGGACATTT	9	0.11202389843166542	No Hit
GTGTGTATTTCTCATTTTCCGTGAT	9	0.11202389843166542	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	9	0.11202389843166542	No Hit
CATCTAAAATGTTCTACAGTGTGGT	9	0.11202389843166542	No Hit
ATTTAGAAATGTCCACTGTAGGACA	9	0.11202389843166542	No Hit
GTAGAACAGTGTATATCAATGAGTT	9	0.11202389843166542	No Hit
GTGGAATATGGCAAGAAAACTGAAA	9	0.11202389843166542	No Hit
GATTAGATGAGTGAGTTACACTGAA	9	0.11202389843166542	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	9	0.11202389843166542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.012447099825740602	0.0
10	0.0	0.0	0.0	0.012447099825740602	0.0
11	0.0	0.0	0.0	0.012447099825740602	0.0
12	0.0	0.0	0.0	0.012447099825740602	0.0
13	0.0	0.0	0.0	0.012447099825740602	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACATG	25	0.0055342307	19.0	6
ATATGGC	100	0.0023095235	8.549999	15
AATATGG	110	0.005477367	7.7727275	14
>>END_MODULE