FastQCFastQC Report
Thu 2 Feb 2017
SRR4064191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8034
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA370.4605426935524023No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA310.38586009459795867No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA310.38586009459795867No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC260.3236245954692557No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG240.2987303958177745No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA220.27383619616629323No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA200.24894199651481203No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA160.19915359721184964No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC150.18670649738610903No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC140.17425939756036843No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT130.16181229773462785No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA120.14936519790888725No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC120.14936519790888725No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATG110.13691809808314662No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA100.12447099825740601No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCAC90.11202389843166542No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCATA90.11202389843166542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA250.002216272835.21
CTAAAGT250.002216272835.24
AGGACGT352.943219E-431.4285725
TATTTCA352.943219E-431.42857238
GGACGTG352.943219E-431.4285726
TCCTAAA300.00541475829.3333322
CCTAAAG300.00541475829.3333323
TCACGTC300.00541475829.33333242
TAGGACG406.436471E-427.5000024
ATATTTC406.436471E-427.50000237
ATTTCAC406.436471E-427.50000239
TTCCACC500.002365168622.011
CCACCTT550.004112431320.013
CTCATTT707.201994E-418.85714319
CACCTTT600.006803192718.33333214
TCCACCT600.006803192718.33333212
CTGTAGG600.006803192718.3333321
AATATGG1104.587724E-516.014
TCATTTT900.003877278914.66666820
ATATGGC1251.3681891E-414.0815
Produced by FastQC (version 0.11.5)