##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064191_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8034
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.04232013940752	32.0	32.0	32.0	21.0	32.0
2	30.03659447348768	32.0	32.0	32.0	21.0	32.0
3	31.05663430420712	32.0	32.0	32.0	32.0	32.0
4	31.301219815782922	32.0	32.0	32.0	32.0	32.0
5	31.03510082150859	32.0	32.0	32.0	32.0	32.0
6	34.58576051779935	36.0	36.0	36.0	32.0	36.0
7	34.6899427433408	36.0	36.0	36.0	32.0	36.0
8	34.51555887478217	36.0	36.0	36.0	32.0	36.0
9	34.80134428678118	36.0	36.0	36.0	32.0	36.0
10	34.33457804331591	36.0	36.0	36.0	32.0	36.0
11	34.93054518297237	36.0	36.0	36.0	36.0	36.0
12	34.76985312422206	36.0	36.0	36.0	32.0	36.0
13	34.78043315907394	36.0	36.0	36.0	32.0	36.0
14	34.612023898431666	36.0	36.0	36.0	32.0	36.0
15	34.49029126213592	36.0	36.0	36.0	32.0	36.0
16	34.65670898680607	36.0	36.0	36.0	32.0	36.0
17	34.72031366691561	36.0	36.0	36.0	32.0	36.0
18	34.75553895942245	36.0	36.0	36.0	32.0	36.0
19	34.65148120487926	36.0	36.0	36.0	32.0	36.0
20	34.59111277072442	36.0	36.0	36.0	32.0	36.0
21	34.72242967388598	36.0	36.0	36.0	32.0	36.0
22	34.65098332088623	36.0	36.0	36.0	32.0	36.0
23	34.53734129947722	36.0	36.0	36.0	32.0	36.0
24	34.63380632312671	36.0	36.0	36.0	32.0	36.0
25	34.53012198157829	36.0	36.0	36.0	32.0	36.0
26	34.449464774707494	36.0	36.0	36.0	32.0	36.0
27	34.42718446601942	36.0	36.0	36.0	32.0	36.0
28	34.21894448593478	36.0	36.0	36.0	32.0	36.0
29	34.1428927059995	36.0	36.0	36.0	32.0	36.0
30	34.09385113268608	36.0	36.0	36.0	32.0	36.0
31	34.03933283544934	36.0	36.0	36.0	32.0	36.0
32	34.03746577047548	36.0	36.0	36.0	32.0	36.0
33	34.16430171769978	36.0	36.0	36.0	32.0	36.0
34	34.14413741598208	36.0	36.0	36.0	32.0	36.0
35	34.05090863828728	36.0	36.0	36.0	32.0	36.0
36	33.96751306945482	36.0	36.0	36.0	32.0	36.0
37	33.822255414488424	36.0	36.0	36.0	32.0	36.0
38	33.74981329350261	36.0	36.0	36.0	27.0	36.0
39	33.35611152601444	36.0	36.0	36.0	21.0	36.0
40	33.60592481951705	36.0	36.0	36.0	21.0	36.0
41	33.562111028130445	36.0	36.0	36.0	21.0	36.0
42	33.5184217077421	36.0	36.0	36.0	21.0	36.0
43	33.474856858352005	36.0	36.0	36.0	21.0	36.0
44	33.38287279063978	36.0	36.0	36.0	21.0	36.0
45	33.279063978093106	36.0	36.0	36.0	21.0	36.0
46	33.06248444112522	36.0	36.0	36.0	14.0	36.0
47	33.226537216828476	36.0	36.0	36.0	21.0	36.0
48	33.01406522280309	36.0	36.0	36.0	14.0	36.0
49	33.05987055016181	36.0	36.0	36.0	14.0	36.0
50	32.60654717450834	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	2.0
24	6.0
25	20.0
26	40.0
27	61.0
28	132.0
29	206.0
30	321.0
31	531.0
32	785.0
33	1166.0
34	1903.0
35	2859.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.843415484192185	23.848643266118994	14.563106796116504	24.744834453572317
2	10.268857356235998	22.666168782673637	49.763505103310926	17.301468757779435
3	20.923574807069954	25.964650236494897	33.70674632810555	19.4050286283296
4	11.127707244212099	20.03983071944237	41.511077918844904	27.321384117500624
5	11.090365944734877	40.16679113766492	37.25416977844162	11.488673139158575
6	28.03086880756784	42.481951705252676	18.247448344535723	11.239731142643764
7	24.209609161065472	32.10107045058501	23.58725416977844	20.102066218571075
8	19.093851132686083	49.19093851132686	19.79088872292756	11.924321633059497
9	25.24271844660194	14.239482200647249	21.04804580532736	39.46975354742345
10	14.438635797859098	30.61986557132188	37.50311177495643	17.438386855862582
11	33.17152103559871	18.222554144884242	31.86457555389594	16.74134926562111
12	24.98132935026139	21.86955439382624	37.64002987303958	15.509086382872791
13	30.682101070450585	24.085138162808065	26.47498132935026	18.757779437391086
14	16.915608663181477	26.985312422205627	26.972865322379885	29.126213592233007
15	17.724670151854617	44.946477470749315	21.50858849887976	15.820263878516306
16	16.56708986806074	28.827483196415237	36.18371919342793	18.421707742096093
17	16.69156086631815	31.964152352501866	33.30843913368185	18.035847647498134
18	17.99850634802091	27.595220313666918	40.390838934528254	14.01543440378392
19	24.645257654966393	23.711725168035848	29.213343291013196	22.429673885984567
20	22.467015185461786	31.814787154592977	30.383370674632808	15.334826985312421
21	20.6621857107294	26.462534229524522	27.24670151854618	25.628578541199897
22	21.86955439382624	35.051033109285534	28.329599203385612	14.749813293502614
23	18.25989544436146	33.54493403037092	30.458053273587254	17.73711725168036
24	23.263629574309185	28.802588996763756	31.62808065720687	16.30570077172019
25	20.774209609161066	30.9932785660941	32.847896440129446	15.384615384615385
26	14.177246701518545	33.283544934030374	35.1630570077172	17.37615135673388
27	17.264127458302216	31.802340054767242	33.86855862584018	17.064973861090365
28	15.934271131582223	34.24623428358023	29.627785385285698	20.19170919955185
29	18.521284540702016	28.366940502862832	28.205128205128204	24.906646751306948
30	16.181229773462782	31.74010455563854	30.20911127707244	21.86955439382624
31	21.010704505850136	27.532984814538214	30.96838436644262	20.48792631316903
32	18.869803335822755	32.511824744834456	31.60318645755539	17.015185461787404
33	16.679113766492407	32.68608414239482	34.77719691311925	15.857605177993527
34	19.01916853373164	32.462036345531494	33.59472242967389	14.924072691062982
35	16.1563355738113	34.42867811799851	31.914363953198905	17.50062235499129
36	15.235250186706498	32.88523773960667	32.95992033856112	18.919591735125714
37	15.971617079546869	33.02626665006847	28.656790738204908	22.345325532179757
38	19.60418222554145	34.60293751555887	28.0806572068708	17.71222305202888
39	22.7159571819766	26.997759522031366	30.196664177246703	20.089619118745333
40	25.093353248693056	28.59098829972616	30.918595967139655	15.397062484441125
41	21.27209360219069	26.12646253422952	35.59870550161812	17.002738361961665
42	19.36768732885238	34.46601941747573	31.889469753547424	14.276823500124472
43	14.426188698033357	34.802091112770725	34.366442618869804	16.405277570326113
44	14.575553895942244	33.91834702514314	30.81901916853373	20.68707991038088
45	15.03609658949465	34.20463032113518	31.665421956684092	19.093851132686083
46	19.25566343042071	27.869056509833207	30.682101070450585	22.193178989295497
47	22.382671480144403	29.216979957674592	30.59878003236649	17.801568529814517
48	17.861588249937764	29.28802588996764	29.449838187702266	23.40054767239233
49	20.21409011700274	29.039083893452826	32.200647249190936	18.546178740353497
50	18.77022653721683	34.67762011451332	27.172018919591736	19.380134428678115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	2.0
6	3.0
7	2.5
8	2.0
9	1.5
10	1.0
11	2.5
12	4.0
13	5.0
14	6.0
15	14.5
16	23.0
17	18.0
18	13.0
19	18.5
20	24.0
21	29.5
22	35.0
23	46.0
24	57.0
25	76.0
26	95.0
27	187.5
28	280.0
29	369.0
30	458.0
31	559.0
32	660.0
33	829.0
34	998.0
35	1173.5
36	1349.0
37	1262.0
38	1175.0
39	1002.5
40	830.0
41	656.5
42	483.0
43	375.0
44	267.0
45	240.0
46	213.0
47	192.5
48	172.0
49	161.0
50	150.0
51	141.5
52	133.0
53	128.0
54	123.0
55	109.5
56	96.0
57	95.0
58	94.0
59	79.5
60	65.0
61	58.0
62	51.0
63	47.5
64	44.0
65	39.5
66	35.0
67	31.0
68	27.0
69	24.0
70	21.0
71	15.5
72	10.0
73	10.0
74	10.0
75	9.0
76	8.0
77	7.5
78	7.0
79	4.0
80	1.0
81	1.0
82	1.0
83	2.0
84	3.0
85	3.0
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.012447099825740602
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.012447099825740602
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.012447099825740602
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	8034.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.05078416728902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.27480916030534	83.00970873786407
2	3.548204693242861	6.248444112521782
3	0.9895391574780887	2.6138909634055265
4	0.49476957873904437	1.7425939756036841
5	0.14136273678258413	0.6223549912870301
6	0.11309018942606729	0.597460791635549
7	0.1272264631043257	0.784167289021658
8	0.07068136839129206	0.49788399302962405
9	0.02827254735651682	0.22404779686333084
>10	0.21204410517387615	3.6594473487677375
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	37	0.4605426935524023	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	31	0.38586009459795867	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	31	0.38586009459795867	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	26	0.3236245954692557	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	24	0.2987303958177745	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	22	0.27383619616629323	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	20	0.24894199651481203	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	16	0.19915359721184964	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	15	0.18670649738610903	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC	14	0.17425939756036843	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	13	0.16181229773462785	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	12	0.14936519790888725	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	12	0.14936519790888725	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATG	11	0.13691809808314662	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	10	0.12447099825740601	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCAC	9	0.11202389843166542	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCATA	9	0.11202389843166542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.024894199651481205	0.0
33	0.0	0.0	0.0	0.024894199651481205	0.0
34	0.0	0.0	0.0	0.024894199651481205	0.0
35	0.0	0.0	0.0	0.024894199651481205	0.0
36	0.0	0.0	0.0	0.024894199651481205	0.0
37	0.0	0.0	0.0	0.024894199651481205	0.0
38	0.0	0.0	0.0	0.06223549912870301	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAA	25	0.0022162728	35.2	1
CTAAAGT	25	0.0022162728	35.2	4
AGGACGT	35	2.943219E-4	31.428572	5
TATTTCA	35	2.943219E-4	31.428572	38
GGACGTG	35	2.943219E-4	31.428572	6
TCCTAAA	30	0.005414758	29.333332	2
CCTAAAG	30	0.005414758	29.333332	3
TCACGTC	30	0.005414758	29.333332	42
TAGGACG	40	6.436471E-4	27.500002	4
ATATTTC	40	6.436471E-4	27.500002	37
ATTTCAC	40	6.436471E-4	27.500002	39
TTCCACC	50	0.0023651686	22.0	11
CCACCTT	55	0.0041124313	20.0	13
CTCATTT	70	7.201994E-4	18.857143	19
CACCTTT	60	0.0068031927	18.333332	14
TCCACCT	60	0.0068031927	18.333332	12
CTGTAGG	60	0.0068031927	18.333332	1
AATATGG	110	4.587724E-5	16.0	14
TCATTTT	90	0.0038772789	14.666668	20
ATATGGC	125	1.3681891E-4	14.08	15
>>END_MODULE