FastQCFastQC Report
Thu 2 Feb 2017
SRR4064190_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064190_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637867
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29590.4638898077498914No Hit
TATCAACGCAGAGTACTTTTTTTTT21580.3383150405962371No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20550.3221674737837198No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12700.19910106652327209No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12080.1893811719370966No Hit
GGTATCAACGCAGAGTACTTTTTTT11330.17762323493769078No Hit
GTACATGGGGTGGTATCAACGCAAA10510.16476789048500706No Hit
GTATCAACGCAGAGTACATGGGGTG7300.1144439201275501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAGG402.756521E-416.6260991
CGTTATT601.4660855E-615.8306522
ACTCTAA1200.015.0414810
CTTGCTC456.759241E-414.7764363
GCGTTAT653.36228E-614.6163491
TAATACC400.00527776214.2487064
CGCAATA400.005280510514.2475872
GGACCTT759.648302E-713.9331616
TCCCCGA759.648302E-713.93316113
GGCGAGG1101.8553692E-1013.81909619
CGGTCCA903.6259735E-813.72205310
ATCCCCG852.6909765E-713.41159912
TTAATTC500.001498880613.2987923
CGAGCCG1153.7471182E-1013.21826515
CCGCCTG1207.348717E-1012.66750219
CACTCTA1352.7284841E-1112.666519
AGGCCCG1207.366907E-1012.6665110
CGTCGTA751.4755329E-512.6665110
TATTATA604.0919843E-412.6645222
GCCGCCT1301.9645086E-1012.42389718
Produced by FastQC (version 0.11.5)