##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064190_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 637867 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.17815782913993 32.0 32.0 32.0 32.0 32.0 2 30.754740408266926 32.0 32.0 32.0 32.0 32.0 3 30.786685939231845 32.0 32.0 32.0 32.0 32.0 4 30.812785423920662 32.0 32.0 32.0 32.0 32.0 5 30.704582616752393 32.0 32.0 32.0 32.0 32.0 6 34.35482631959327 36.0 36.0 36.0 32.0 36.0 7 34.258150993859225 36.0 36.0 36.0 32.0 36.0 8 34.220549111335124 36.0 36.0 36.0 32.0 36.0 9 34.367614251873825 36.0 36.0 36.0 32.0 36.0 10 34.049991612671604 36.0 36.0 36.0 32.0 36.0 11 34.37206188750947 36.0 36.0 36.0 32.0 36.0 12 34.157587710290706 36.0 36.0 36.0 32.0 36.0 13 34.24883400458089 36.0 36.0 36.0 32.0 36.0 14 34.15086687350184 36.0 36.0 36.0 32.0 36.0 15 34.09299744304063 36.0 36.0 36.0 32.0 36.0 16 34.0915817874259 36.0 36.0 36.0 32.0 36.0 17 34.01255120581563 36.0 36.0 36.0 32.0 36.0 18 33.99582985167754 36.0 36.0 36.0 32.0 36.0 19 34.01129702586903 36.0 36.0 36.0 32.0 36.0 20 33.98478522952277 36.0 36.0 36.0 32.0 36.0 21 33.96856554736332 36.0 36.0 36.0 32.0 36.0 22 33.94482078552426 36.0 36.0 36.0 32.0 36.0 23 33.908317878178366 36.0 36.0 36.0 32.0 36.0 24 33.88378925387267 36.0 36.0 36.0 32.0 36.0 25 33.49499190270072 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 8.0 5 5.0 6 32.0 7 13.0 8 37.0 9 42.0 10 61.0 11 23.0 12 41.0 13 32.0 14 93.0 15 128.0 16 215.0 17 276.0 18 351.0 19 494.0 20 695.0 21 1015.0 22 1680.0 23 2545.0 24 3725.0 25 5418.0 26 7719.0 27 10091.0 28 13854.0 29 18276.0 30 24451.0 31 32854.0 32 46241.0 33 64934.0 34 135491.0 35 267023.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56146018920479 18.175516298210837 12.205935861082533 25.057087651501845 2 15.87946631547591 20.78014517975009 38.45664205195742 24.88374645281658 3 18.410499053065934 25.48177622254832 29.138760331615053 26.96896439277069 4 12.400346797065707 16.149894095199873 36.7614296442508 34.68832946348362 5 14.0620884289746 37.35340232047663 33.60175603637504 14.982753214173721 6 32.140633991304064 36.405814644487265 17.935177156619403 13.518374207589268 7 28.74761841290252 31.538877694231658 21.605758148360135 18.10774574450569 8 27.452613287412998 32.969954173763306 20.338568377073706 19.23886416174999 9 27.78619560307183 14.409068664357777 19.463842041861167 38.34089369070923 10 16.137108400093492 27.171633350641038 31.954207262647827 24.73705098661764 11 35.81652677102238 21.764426297139288 23.49337018567872 18.925676746159603 12 24.782919042725926 24.314636002102176 29.511244283730893 21.39120067144101 13 29.341564431614948 20.45699852530514 25.71836465752565 24.483072385554266 14 23.367937508530765 20.457515622082873 26.39822152215488 29.77632534723148 15 24.401454518073233 28.06473170165437 23.48469398960252 24.049119790669877 16 24.272242207833976 26.326515563936788 25.467090678895453 23.934151549333784 17 22.87440818516392 26.612293433482993 26.26622490375655 24.247073477596533 18 23.54455762459754 25.46506373444296 27.881700589331775 23.108678051627724 19 24.944772531865624 25.126929038979295 26.723818728387545 23.204479700767536 20 25.558381568191198 24.541173923208117 26.217534632601797 23.682909875998888 21 26.269916607175077 24.507919572601967 25.53499282178412 23.687170998438837 22 25.430228325404496 25.07029259291802 25.87834360525688 23.6211354764206 23 23.633259637125942 25.108700598304413 26.590888763708197 24.667151000861445 24 24.101497164769714 25.424970737661962 26.76946926120834 23.704062836359984 25 24.090292901209388 25.26610266290943 26.731863911081543 23.911740524799637 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 611.0 1 611.0 2 684.5 3 758.0 4 758.0 5 758.0 6 1704.5 7 2651.0 8 2651.0 9 2651.0 10 2529.0 11 2407.0 12 2407.0 13 2407.0 14 2319.0 15 2231.0 16 2231.0 17 2231.0 18 3803.5 19 5376.0 20 5376.0 21 5376.0 22 8666.5 23 11957.0 24 11957.0 25 11957.0 26 17562.0 27 23167.0 28 23167.0 29 23167.0 30 28746.5 31 34326.0 32 34326.0 33 34326.0 34 39881.5 35 45437.0 36 45437.0 37 45437.0 38 50381.5 39 55326.0 40 55326.0 41 55326.0 42 62141.5 43 68957.0 44 68957.0 45 68957.0 46 76711.5 47 84466.0 48 84466.0 49 84466.0 50 84549.0 51 84632.0 52 84632.0 53 84632.0 54 76497.5 55 68363.0 56 68363.0 57 68363.0 58 61515.5 59 54668.0 60 54668.0 61 54668.0 62 47395.0 63 40122.0 64 40122.0 65 40122.0 66 32723.5 67 25325.0 68 25325.0 69 25325.0 70 19057.5 71 12790.0 72 12790.0 73 12790.0 74 9803.5 75 6817.0 76 6817.0 77 6817.0 78 5490.0 79 4163.0 80 4163.0 81 4163.0 82 2855.0 83 1547.0 84 1547.0 85 1547.0 86 1201.0 87 855.0 88 855.0 89 855.0 90 617.5 91 380.0 92 380.0 93 380.0 94 270.0 95 160.0 96 160.0 97 160.0 98 267.5 99 375.0 100 375.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03934989582467818 2 0.005800582253040211 3 0.002978677373182811 4 0.004703174799762333 5 0.010503757052802544 6 0.015363704345890287 7 0.0253971439187166 8 0.03778217089142408 9 0.051891695290711075 10 0.06051418242360868 11 0.05769227754375128 12 0.06772571711657759 13 0.07007730451645876 14 0.07336952687629239 15 0.06349285979679149 16 0.07274243690299075 17 0.06600121968999807 18 0.08387328392909493 19 0.07885656414268177 20 0.08371651143576953 21 0.08183524151586459 22 0.08324619395579329 23 0.08873323122218268 24 0.08293264896914247 25 0.08136492403588835 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 637867.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.11449720022352 #Duplication Level Percentage of deduplicated Percentage of total 1 81.24799108236101 48.84182132444376 2 11.961367923155496 14.381032370547487 3 3.2721381159873753 5.9010881283680305 4 1.2055814795858986 2.898916979168313 5 0.5821687518783569 1.749839090242455 6 0.36281546650944957 1.3086281607408057 7 0.2321882901294575 0.977051762183837 8 0.17860808866170702 0.8589548356633168 9 0.11652833656675811 0.6304538126060175 >10 0.6744195211666215 7.759724473839369 >50 0.08991198751686795 3.8318862761088264 >100 0.07287321615712047 8.451985476629075 >500 0.0015728032264094342 0.5438101625739158 >1k 0.0018349370974776733 1.8648071468848793 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2959 0.4638898077498914 No Hit TATCAACGCAGAGTACTTTTTTTTT 2158 0.3383150405962371 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2055 0.3221674737837198 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1270 0.19910106652327209 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1208 0.1893811719370966 No Hit GGTATCAACGCAGAGTACTTTTTTT 1133 0.17762323493769078 No Hit GTACATGGGGTGGTATCAACGCAAA 1051 0.16476789048500706 No Hit GTATCAACGCAGAGTACATGGGGTG 730 0.1144439201275501 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.567724933254111E-4 10 0.0 0.0 0.0 0.0 3.135449866508222E-4 11 0.0 0.0 0.0 0.0 3.135449866508222E-4 12 0.0 0.0 0.0 0.0 3.135449866508222E-4 13 0.0 0.0 0.0 0.0 3.135449866508222E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTAGG 40 2.756521E-4 16.626099 1 CGTTATT 60 1.4660855E-6 15.830652 2 ACTCTAA 120 0.0 15.04148 10 CTTGCTC 45 6.759241E-4 14.776436 3 GCGTTAT 65 3.36228E-6 14.616349 1 TAATACC 40 0.005277762 14.248706 4 CGCAATA 40 0.0052805105 14.247587 2 GGACCTT 75 9.648302E-7 13.933161 6 TCCCCGA 75 9.648302E-7 13.933161 13 GGCGAGG 110 1.8553692E-10 13.819096 19 CGGTCCA 90 3.6259735E-8 13.722053 10 ATCCCCG 85 2.6909765E-7 13.411599 12 TTAATTC 50 0.0014988806 13.298792 3 CGAGCCG 115 3.7471182E-10 13.218265 15 CCGCCTG 120 7.348717E-10 12.667502 19 CACTCTA 135 2.7284841E-11 12.66651 9 AGGCCCG 120 7.366907E-10 12.66651 10 CGTCGTA 75 1.4755329E-5 12.66651 10 TATTATA 60 4.0919843E-4 12.664522 2 GCCGCCT 130 1.9645086E-10 12.423897 18 >>END_MODULE