FastQCFastQC Report
Thu 2 Feb 2017
SRR4064190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637867
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9920.1555183133788078No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7880.12353672474042394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT651.8133987E-523.6893521
GTATATA755.472695E-520.5307711
TATAGTG1101.2628698E-720.000645
TATCTAG1252.2861059E-819.3575861
GCATTCG2350.018.72400322
CGTATTG2400.018.33535827
ACCCTCG600.007403640518.33535824
ATAATGC851.4299985E-418.1168083
CCTTAGG1102.7955539E-617.9977532
GACGGCC2450.017.95694411
CCGCTAG2350.017.78919836
GCCGCTA2350.017.78919835
CGCTAGA2350.017.78919837
TTCGTAT2550.017.25545525
TAAACGC902.2114391E-417.11328
AACGCAG24600.016.9924146
TAAGAGG2850.016.9834
ATTCGTA2600.016.92494824
CGCCCGA1307.301005E-716.9236226
GTATCAA25050.016.8601721
Produced by FastQC (version 0.11.5)