##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	637867
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2308302514474	32.0	32.0	32.0	32.0	32.0
2	31.39985294740126	32.0	32.0	32.0	32.0	32.0
3	31.469093086803362	32.0	32.0	32.0	32.0	32.0
4	31.55243334425515	32.0	32.0	32.0	32.0	32.0
5	31.49092052104906	32.0	32.0	32.0	32.0	32.0
6	35.06673021178396	36.0	36.0	36.0	36.0	36.0
7	35.08616843323138	36.0	36.0	36.0	36.0	36.0
8	35.04749109140307	36.0	36.0	36.0	36.0	36.0
9	35.148653245896085	36.0	36.0	36.0	36.0	36.0
10	35.0105648983252	36.0	36.0	36.0	36.0	36.0
11	35.1391355878263	36.0	36.0	36.0	36.0	36.0
12	35.043772447861386	36.0	36.0	36.0	36.0	36.0
13	35.087675016892234	36.0	36.0	36.0	36.0	36.0
14	35.03809728360301	36.0	36.0	36.0	36.0	36.0
15	35.012113810559256	36.0	36.0	36.0	36.0	36.0
16	35.023417107328015	36.0	36.0	36.0	36.0	36.0
17	34.98075147326951	36.0	36.0	36.0	36.0	36.0
18	34.9762489672612	36.0	36.0	36.0	36.0	36.0
19	34.9703935146355	36.0	36.0	36.0	36.0	36.0
20	34.9722089401082	36.0	36.0	36.0	36.0	36.0
21	34.953861855214335	36.0	36.0	36.0	36.0	36.0
22	34.943605798700986	36.0	36.0	36.0	36.0	36.0
23	34.89375528127337	36.0	36.0	36.0	32.0	36.0
24	34.87269916769483	36.0	36.0	36.0	32.0	36.0
25	34.852129048845605	36.0	36.0	36.0	32.0	36.0
26	34.78607609423281	36.0	36.0	36.0	32.0	36.0
27	34.78843552025736	36.0	36.0	36.0	32.0	36.0
28	34.757857045434235	36.0	36.0	36.0	32.0	36.0
29	34.71446712245656	36.0	36.0	36.0	32.0	36.0
30	34.68964846903822	36.0	36.0	36.0	32.0	36.0
31	34.679240343206345	36.0	36.0	36.0	32.0	36.0
32	34.6247493599763	36.0	36.0	36.0	32.0	36.0
33	34.57999081313189	36.0	36.0	36.0	32.0	36.0
34	34.560134009127296	36.0	36.0	36.0	32.0	36.0
35	34.500187343129525	36.0	36.0	36.0	32.0	36.0
36	34.476384575467925	36.0	36.0	36.0	32.0	36.0
37	34.447659151515914	36.0	36.0	36.0	32.0	36.0
38	34.40067443526629	36.0	36.0	36.0	32.0	36.0
39	34.38537343991773	36.0	36.0	36.0	32.0	36.0
40	34.35390136188265	36.0	36.0	36.0	32.0	36.0
41	34.331669454604175	36.0	36.0	36.0	32.0	36.0
42	34.24720984155004	36.0	36.0	36.0	32.0	36.0
43	34.22723545817545	36.0	36.0	36.0	32.0	36.0
44	34.146634016182055	36.0	36.0	36.0	32.0	36.0
45	34.11087891362933	36.0	36.0	36.0	32.0	36.0
46	34.04182533349429	36.0	36.0	36.0	32.0	36.0
47	33.995930186073274	36.0	36.0	36.0	32.0	36.0
48	33.93522160575794	36.0	36.0	36.0	32.0	36.0
49	33.87261921372324	36.0	36.0	36.0	32.0	36.0
50	33.30989061983141	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	9.0
21	34.0
22	88.0
23	223.0
24	613.0
25	1446.0
26	2875.0
27	5219.0
28	8435.0
29	12944.0
30	17548.0
31	24592.0
32	34759.0
33	54119.0
34	109887.0
35	365072.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.71981963195763	19.1857411411289	12.758561759278278	25.335877467635193
2	15.209849136883625	21.605893428526187	37.91362722089226	25.270630213697924
3	17.772726203820557	26.062097779310665	29.497777742939334	26.66739827392944
4	12.119924686494207	16.94679298349029	36.77663211923489	34.15665021078061
5	13.617101997751883	37.78812824617044	33.37749091895332	15.217278837124354
6	32.27381256594243	36.6286388855357	17.552718670193002	13.544829878328867
7	28.551563256917195	31.483522427089035	21.77303419051307	18.191880125480704
8	27.433775379507015	33.33030239846237	20.181166293286847	19.054755928743766
9	27.75348629390692	14.185355605897893	19.424007023539833	38.63715107665535
10	15.89815224173557	27.412072355115747	31.988197926171193	24.70157747697749
11	35.95802253137806	21.710202456315276	23.46731131616985	18.864463696136806
12	24.7214932289854	24.38129638513418	29.52171772754779	21.37549265833263
13	29.454711349127717	20.457182095242874	25.802992487426785	24.285114068202628
14	23.450029551614993	20.382148629730022	26.34263882596215	29.825182992692834
15	24.299929918520483	28.15503841938827	23.54627964548492	23.998752016606332
16	24.207311624319406	26.321251808779657	25.458211114960783	24.01322545194016
17	23.023921914756524	26.497059731887685	26.26143067441959	24.217587678936205
18	23.624084055271798	25.329144365744526	28.10402184784892	22.94274973113475
19	24.934665063406637	24.972290461804732	26.9518567350247	23.141187739763932
20	25.51155105163483	24.520901007111235	26.536377660441723	23.43117028081221
21	26.276637606272153	24.38799937918093	25.796286686723096	23.539076327823825
22	25.33400432065669	24.95398720107358	26.135583335684924	23.576425142584807
23	23.46447977775757	24.863214927366734	27.053030279276708	24.619275015598983
24	24.146764367437676	25.302493000097197	26.90753230302289	23.643210329442237
25	24.08897933075804	25.10707745948929	26.93397882907741	23.869964380675263
26	23.531551419218083	25.88175900259636	27.302388149560375	23.284301428625184
27	24.20499602556806	25.451572291546142	26.503111345239383	23.840320337646414
28	23.410139549322007	25.573224591158585	27.013713491962704	24.002922367556703
29	23.663548881704987	25.325862355490198	26.87623658375219	24.134352179052623
30	23.494652146510177	25.535334087980104	27.194720817276185	23.77529294823353
31	24.14345115648989	25.33615975114144	26.377408308664897	24.142980783703777
32	23.788687149305236	25.802511911275694	26.112463764480488	24.296337174938582
33	23.329581050869034	25.247321403374546	26.932085777804254	24.491011767952166
34	24.139752916091812	25.502320331117524	26.863633513106734	23.494293239683934
35	24.530567771166975	25.49145952386552	26.720441486568053	23.257531218399453
36	23.54836433762141	26.030118451275058	26.468490079411744	23.953027131691794
37	24.358612558714153	25.97768078314802	26.084604820047787	23.57910183809004
38	23.716562741553375	25.88966116284284	26.45645414934799	23.937321946255796
39	24.429006829341713	25.473475940523898	26.04509623163321	24.05242099850118
40	24.75315513051658	25.759347809046012	26.406051579524963	23.08144548091244
41	23.714180000689826	25.89575474804105	26.82106226933943	23.569002981929692
42	24.48118273248203	26.557392469957435	26.193981296396462	22.767443501164074
43	23.76362578838698	25.758442776000113	26.45766344283087	24.020267992782035
44	23.405512860309727	26.24095796216689	26.029781109783745	24.32374806773964
45	23.73866119833065	26.340353748663485	25.90718252139204	24.013802531613816
46	23.42045317367372	25.930576916142645	26.082021518929793	24.56694839125384
47	24.07120852244668	25.787299422273435	26.035165283101698	24.106326772178193
48	24.158657991690834	26.397899192600143	25.361762169789138	24.081680645919885
49	23.880049477709164	26.339511776039682	25.498108528006796	24.282330218244358
50	23.610584173907046	27.06417415788467	25.32130975859831	24.003931909609978
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	286.0
1	172.5
2	59.0
3	180.5
4	302.0
5	283.5
6	265.0
7	258.0
8	251.0
9	363.0
10	475.0
11	723.5
12	972.0
13	1633.5
14	2295.0
15	2964.5
16	3634.0
17	3760.5
18	3887.0
19	3871.0
20	3855.0
21	4077.0
22	4299.0
23	4733.0
24	5167.0
25	5291.0
26	5415.0
27	6332.5
28	7250.0
29	8635.5
30	10021.0
31	11816.0
32	13611.0
33	15980.5
34	18350.0
35	21045.5
36	23741.0
37	26076.0
38	28411.0
39	29503.0
40	30595.0
41	32387.5
42	34180.0
43	34612.5
44	35045.0
45	38039.0
46	41033.0
47	43146.0
48	45259.0
49	46212.0
50	47165.0
51	45415.0
52	43665.0
53	42704.0
54	41743.0
55	41451.0
56	41159.0
57	39352.0
58	37545.0
59	34255.5
60	30966.0
61	27039.5
62	23113.0
63	19905.5
64	16698.0
65	14170.5
66	11643.0
67	9981.0
68	8319.0
69	7371.5
70	6424.0
71	5049.0
72	3674.0
73	3173.0
74	2672.0
75	2103.5
76	1535.0
77	1369.0
78	1203.0
79	965.0
80	727.0
81	574.0
82	421.0
83	354.0
84	287.0
85	206.5
86	126.0
87	90.0
88	54.0
89	38.0
90	22.0
91	20.0
92	18.0
93	12.5
94	7.0
95	11.0
96	15.0
97	10.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009406349599524666
2	6.270899733016444E-4
3	3.135449866508222E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0018812699199049331
10	0.0020380424132303444
11	1.567724933254111E-4
12	1.567724933254111E-4
13	4.703174799762333E-4
14	0.0
15	0.005957354746365622
16	9.406349599524666E-4
17	0.0
18	1.567724933254111E-4
19	0.0
20	4.703174799762333E-4
21	0.0
22	1.567724933254111E-4
23	7.838624666270555E-4
24	1.567724933254111E-4
25	0.0017244974265795219
26	0.007995397159595966
27	0.006270899733016444
28	0.00438962981311151
29	0.0032922223598336334
30	0.006427672226341856
31	0.011757936999405832
32	0.004076084826460689
33	0.0051734922797385665
34	0.004232857319786099
35	0.0034489948531590438
36	0.008152169652921377
37	0.004859947293087744
38	0.010660529546127956
39	0.004859947293087744
40	0.002665132386531989
41	0.0039193123331352775
42	0.0034489948531590438
43	0.002194814906555755
44	0.0020380424132303444
45	0.0020380424132303444
46	0.001567724933254111
47	0.0034489948531590438
48	0.002665132386531989
49	6.270899733016444E-4
50	0.0014109524399286998
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	637867.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.15079388102428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60679937202961	53.81898559058228
2	11.415299824377021	14.874316918961602
3	2.904135712939587	5.676202417087456
4	1.0941921910541343	2.8514995962237704
5	0.5333269496304347	1.7373337083283935
6	0.32133585686105876	1.2561171706162297
7	0.20414418356324454	0.9310108937737251
8	0.14192637572927735	0.7397292841135166
9	0.10082337749298068	0.5911850776890498
>10	0.5511637398714517	6.847547655472612
>50	0.06996254325655688	3.2840499246071886
>100	0.054469027526852926	6.433557218490985
>500	0.0024208456678282766	0.9584645440531324
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	992	0.1555183133788078	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	788	0.12353672474042394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.567724933254111E-4
13	0.0	0.0	0.0	0.0	1.567724933254111E-4
14	0.0	0.0	0.0	0.0	1.567724933254111E-4
15	0.0	0.0	0.0	0.0	3.135449866508222E-4
16	0.0	0.0	0.0	1.567724933254111E-4	4.703174799762333E-4
17	0.0	0.0	0.0	1.567724933254111E-4	4.703174799762333E-4
18	0.0	0.0	0.0	1.567724933254111E-4	4.703174799762333E-4
19	0.0	0.0	0.0	1.567724933254111E-4	4.703174799762333E-4
20	0.0	0.0	0.0	1.567724933254111E-4	6.270899733016444E-4
21	0.0	0.0	0.0	3.135449866508222E-4	6.270899733016444E-4
22	0.0	0.0	0.0	7.838624666270554E-4	6.270899733016444E-4
23	0.0	0.0	0.0	0.002978677373182811	7.838624666270554E-4
24	0.0	0.0	0.0	0.005330264773063977	7.838624666270554E-4
25	0.0	0.0	0.0	0.007368307186294321	7.838624666270554E-4
26	0.0	0.0	0.0	0.00877925962622302	7.838624666270554E-4
27	0.0	0.0	0.0	0.010817302039453365	7.838624666270554E-4
28	0.0	0.0	0.0	0.01740174675912063	7.838624666270554E-4
29	0.0	0.0	0.0	0.02884613877187564	7.838624666270554E-4
30	0.0	0.0	0.0	0.05345942022396519	7.838624666270554E-4
31	0.0	0.0	0.0	0.09531767594184995	7.838624666270554E-4
32	0.0	0.0	0.0	0.13874365659298882	9.406349599524666E-4
33	0.0	0.0	0.0	0.1782503249109924	9.406349599524666E-4
34	0.0	0.0	0.0	0.22559561789526658	9.406349599524666E-4
35	0.0	0.0	0.0	0.2762331332393744	9.406349599524666E-4
36	0.0	0.0	0.0	0.3463104377558331	9.406349599524666E-4
37	0.0	0.0	0.0	0.45040737332390607	9.406349599524666E-4
38	0.0	0.0	0.0	0.5795879078240448	9.406349599524666E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAAT	65	1.8133987E-5	23.689352	1
GTATATA	75	5.472695E-5	20.530771	1
TATAGTG	110	1.2628698E-7	20.00064	5
TATCTAG	125	2.2861059E-8	19.357586	1
GCATTCG	235	0.0	18.724003	22
CGTATTG	240	0.0	18.335358	27
ACCCTCG	60	0.0074036405	18.335358	24
ATAATGC	85	1.4299985E-4	18.116808	3
CCTTAGG	110	2.7955539E-6	17.997753	2
GACGGCC	245	0.0	17.956944	11
CCGCTAG	235	0.0	17.789198	36
GCCGCTA	235	0.0	17.789198	35
CGCTAGA	235	0.0	17.789198	37
TTCGTAT	255	0.0	17.255455	25
TAAACGC	90	2.2114391E-4	17.113	28
AACGCAG	2460	0.0	16.992414	6
TAAGAGG	285	0.0	16.983	4
ATTCGTA	260	0.0	16.924948	24
CGCCCGA	130	7.301005E-7	16.92362	26
GTATCAA	2505	0.0	16.860172	1
>>END_MODULE