FastQCFastQC Report
Thu 2 Feb 2017
SRR4064189_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064189_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100241
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3540.35314891112419067No Hit
TATCAACGCAGAGTACTTTTTTTTT2550.25438692750471364No Hit
GTCCTAAAGTGTGTATTTCTCATTT2160.21548069153340452No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG1740.17358166817968695No Hit
CTTTAGGACGTGAAATATGGCGAGG1700.16959128500314244No Hit
CTGTAGGACGTGGAATATGGCAAGA1640.16360571023832565No Hit
GTCCTACAGTGGACATTTCTAAATT1640.16360571023832565No Hit
GGTATCAACGCAGAGTACTTTTTTT1360.13567302800251393No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1110.11073313314911065No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1110.11073313314911065No Hit
GTCCTACAGTGTGCATTTCTCATTT1070.10674274997256611No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1020.10175477100188546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGT400.00523260114.2503756
GTATCAA1153.601599E-1013.211131
ACGTGAA655.352329E-513.1541928
AGGACGT1155.0713425E-912.391635
GACGTGA701.07108426E-412.2146077
TGAGTTA550.003033457312.09122712
GGACGTG1103.655441E-812.0912276
TATGGCA951.0075328E-612.00031716
CGTGAAA657.907068E-411.69261559
GGCGAGG657.907068E-411.692615519
TTAGGAC907.2650437E-611.6114173
ATGGCAA907.2650437E-611.61141717
TAAAGTG752.0365657E-411.40035
TAGGACG1251.7456841E-811.40034
GTCCTAC752.0460694E-411.3946011
CTAAAGT600.005809602311.0836254
GAGTTAC600.005809602311.08362513
GTGTGCA700.001470044610.8574299
AGTGTGC700.001470044610.8574298
AAATATG1002.3367244E-510.45027413
Produced by FastQC (version 0.11.5)