##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064189_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	100241
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.00543689707804	32.0	32.0	32.0	21.0	32.0
2	28.647768877006413	32.0	32.0	32.0	14.0	32.0
3	28.59554473718339	32.0	32.0	32.0	14.0	32.0
4	28.536596801707883	32.0	32.0	32.0	14.0	32.0
5	27.907512893925638	32.0	32.0	32.0	14.0	32.0
6	31.69618220089584	36.0	32.0	36.0	14.0	36.0
7	31.561656408056585	36.0	32.0	36.0	14.0	36.0
8	31.333905288255306	36.0	32.0	36.0	14.0	36.0
9	31.90678464899592	36.0	32.0	36.0	14.0	36.0
10	30.604742570405325	36.0	32.0	36.0	14.0	36.0
11	32.07225586336928	36.0	32.0	36.0	14.0	36.0
12	31.245099310661306	36.0	32.0	36.0	14.0	36.0
13	31.681088576530563	36.0	32.0	36.0	14.0	36.0
14	31.297652657096396	36.0	32.0	36.0	14.0	36.0
15	31.005766103690107	36.0	32.0	36.0	14.0	36.0
16	31.02331381370896	36.0	32.0	36.0	14.0	36.0
17	30.87637792919065	36.0	32.0	36.0	14.0	36.0
18	30.937839806067377	36.0	32.0	36.0	14.0	36.0
19	30.974980297483064	36.0	32.0	36.0	14.0	36.0
20	31.026146985764306	36.0	32.0	36.0	14.0	36.0
21	30.999830408714995	36.0	32.0	36.0	14.0	36.0
22	30.909478157640088	36.0	32.0	36.0	14.0	36.0
23	30.733352620185354	36.0	32.0	36.0	14.0	36.0
24	30.716642890633572	36.0	32.0	36.0	14.0	36.0
25	30.11628974172245	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	0.0
6	4.0
7	2.0
8	11.0
9	6.0
10	6.0
11	2.0
12	5.0
13	1.0
14	54.0
15	114.0
16	283.0
17	444.0
18	809.0
19	1078.0
20	1372.0
21	1506.0
22	1823.0
23	2076.0
24	2433.0
25	2919.0
26	3593.0
27	4107.0
28	5020.0
29	6171.0
30	7399.0
31	9270.0
32	11038.0
33	12267.0
34	15816.0
35	10611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.81118706651365	18.559952098198693	11.966468739084876	25.662392096202787
2	17.20205909934356	19.359923382350008	40.450727269099545	22.987290249206886
3	20.003790864017716	23.43053241687533	30.39873903891621	26.16693768019074
4	12.856145251396647	15.504788507581804	37.96687948922586	33.67218675179569
5	15.281782176242306	36.15132136835699	34.5900216486926	13.9768748067081
6	33.82917801522453	36.53587141959235	16.939530893019263	12.695419672163858
7	29.298079321526565	30.906460463956098	21.661262160139685	18.134198054377652
8	27.509953997066187	35.146840167247106	19.61461316621928	17.728592669467425
9	27.62364775857251	13.61322901281386	19.4104027783322	39.352720450281424
10	16.21041706170597	26.77019191425235	33.488687737647325	23.530703286394345
11	37.19187241771621	20.577433584159994	24.281950460070657	17.948743538053133
12	25.276216901380337	23.231163853762236	31.023125368039683	20.469493876817744
13	30.360618431165097	20.105001547076025	26.010839513319826	23.52354050843905
14	23.368301276612733	20.366913871061115	26.63219779013245	29.6325870621937
15	25.009480848685655	29.685036226821822	22.76202071814934	22.543462206343186
16	24.572820185244332	26.003074097732355	26.84046630469499	22.58363941232833
17	23.20006387353041	26.944649593804264	27.24705083933811	22.60823569332721
18	23.748016090875517	25.192401752827383	29.588444914704386	21.471137241592718
19	25.9290312921096	24.632430004491688	26.39516893746569	23.043369765933026
20	25.858712903902013	25.497359726090295	26.739136944868687	21.904790425139
21	26.23078458774206	24.320223597524457	25.696745857456577	23.7522459572769
22	25.52779469160819	26.176620317225822	26.024894940158312	22.270690051007676
23	23.962586222386374	25.59669385188217	27.169909260608723	23.27081066512273
24	25.290970433810468	25.177177536882873	26.992872971192426	22.53897905811423
25	25.244307802876794	24.810093730348072	27.1009472854134	22.844651181361737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	11.0
2	33.5
3	56.0
4	56.0
5	56.0
6	125.5
7	195.0
8	195.0
9	195.0
10	248.0
11	301.0
12	301.0
13	301.0
14	396.0
15	491.0
16	491.0
17	491.0
18	752.0
19	1013.0
20	1013.0
21	1013.0
22	1596.0
23	2179.0
24	2179.0
25	2179.0
26	3359.5
27	4540.0
28	4540.0
29	4540.0
30	5790.0
31	7040.0
32	7040.0
33	7040.0
34	7784.5
35	8529.0
36	8529.0
37	8529.0
38	8993.0
39	9457.0
40	9457.0
41	9457.0
42	10111.0
43	10765.0
44	10765.0
45	10765.0
46	11259.0
47	11753.0
48	11753.0
49	11753.0
50	11571.0
51	11389.0
52	11389.0
53	11389.0
54	10334.5
55	9280.0
56	9280.0
57	9280.0
58	8321.5
59	7363.0
60	7363.0
61	7363.0
62	6549.0
63	5735.0
64	5735.0
65	5735.0
66	4748.0
67	3761.0
68	3761.0
69	3761.0
70	3012.5
71	2264.0
72	2264.0
73	2264.0
74	1808.0
75	1352.0
76	1352.0
77	1352.0
78	1121.5
79	891.0
80	891.0
81	891.0
82	734.0
83	577.0
84	577.0
85	577.0
86	506.0
87	435.0
88	435.0
89	435.0
90	368.0
91	301.0
92	301.0
93	301.0
94	280.0
95	259.0
96	259.0
97	259.0
98	281.5
99	304.0
100	304.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03591344858890075
2	0.0029927873824083955
3	0.0
4	9.975957941361318E-4
5	0.003990383176544527
6	0.007980766353089055
7	0.01596153270617811
8	0.02992787382408396
9	0.03691104438303688
10	0.03990383176544528
11	0.03890623597130915
12	0.04788459811853433
13	0.05187498129507886
14	0.05387017288335113
15	0.03890623597130915
16	0.048882193912670464
17	0.04289661914785367
18	0.05985574764816792
19	0.05586536447162339
20	0.061850939236440174
21	0.060853343442304045
22	0.05985574764816792
23	0.06384613082471244
24	0.05885815185403178
25	0.05985574764816792
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	100241.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.3084566195469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.93806365059253	74.63712452988298
2	4.2657325283843885	6.851487914126954
3	1.032272490124469	2.487006314781377
4	0.46831134630195526	1.504374457557287
5	0.28819159772428016	1.157211121197913
6	0.1826041589028844	0.8798794904280683
7	0.11055625947181437	0.6215021797468102
8	0.10807184914660506	0.6943266727187478
9	0.08819656654493056	0.6374637124529883
>10	0.47949119276539715	7.175706547221197
>50	0.02360189808948846	1.2948793407886992
>100	0.01490646195125587	2.0590377190969766
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	354	0.35314891112419067	No Hit
TATCAACGCAGAGTACTTTTTTTTT	255	0.25438692750471364	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	216	0.21548069153340452	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	174	0.17358166817968695	No Hit
CTTTAGGACGTGAAATATGGCGAGG	170	0.16959128500314244	No Hit
CTGTAGGACGTGGAATATGGCAAGA	164	0.16360571023832565	No Hit
GTCCTACAGTGGACATTTCTAAATT	164	0.16360571023832565	No Hit
GGTATCAACGCAGAGTACTTTTTTT	136	0.13567302800251393	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	111	0.11073313314911065	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	111	0.11073313314911065	No Hit
GTCCTACAGTGTGCATTTCTCATTT	107	0.10674274997256611	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	102	0.10175477100188546	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAGTGT	40	0.005232601	14.250375	6
GTATCAA	115	3.601599E-10	13.21113	1
ACGTGAA	65	5.352329E-5	13.154192	8
AGGACGT	115	5.0713425E-9	12.39163	5
GACGTGA	70	1.07108426E-4	12.214607	7
TGAGTTA	55	0.0030334573	12.091227	12
GGACGTG	110	3.655441E-8	12.091227	6
TATGGCA	95	1.0075328E-6	12.000317	16
CGTGAAA	65	7.907068E-4	11.6926155	9
GGCGAGG	65	7.907068E-4	11.6926155	19
TTAGGAC	90	7.2650437E-6	11.611417	3
ATGGCAA	90	7.2650437E-6	11.611417	17
TAAAGTG	75	2.0365657E-4	11.4003	5
TAGGACG	125	1.7456841E-8	11.4003	4
GTCCTAC	75	2.0460694E-4	11.394601	1
CTAAAGT	60	0.0058096023	11.083625	4
GAGTTAC	60	0.0058096023	11.083625	13
GTGTGCA	70	0.0014700446	10.857429	9
AGTGTGC	70	0.0014700446	10.857429	8
AAATATG	100	2.3367244E-5	10.450274	13
>>END_MODULE