FastQCFastQC Report
Thu 2 Feb 2017
SRR4064189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100241
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG2430.24241577797508004No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC1210.12070909109047195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTAGA357.2712864E-637.678361
TAACCCC250.0023477635.1840255
CATGAGT300.005704968729.34932735
ACAATTC300.005732916329.320028
TATTGGA300.00574693129.305392
AACGCAG1450.027.297956
GTATCAA1450.027.2843281
TCAACGC1600.024.7387684
TTAAACC450.001392969624.433354
TGACTTG450.001392969624.4333541
ATCAACG1650.023.9771373
TATACAC752.049921E-623.4443113
CAACGCA1700.023.2835455
TGTAGAA852.3295797E-723.2719272
ACGCAGA1750.022.61837
ACATGAG500.00255598222.01199334
AGACGGA500.002563416722.00140
CATGGGT500.002570869421.99001517
AGAGTAC1800.021.99001511
ACGGACC500.002570869421.99001542
Produced by FastQC (version 0.11.5)