FastQCFastQC Report
Thu 2 Feb 2017
SRR4064188_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064188_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617026
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36550.22603223448478876No Hit
TATCAACGCAGAGTACTTTTTTTTT24220.14978114142877108No Hit
GCGCAAGACGGACCAGAGCGAAAGC18570.11484045401867378No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17850.11038783544605962No Hit
GTATCTGATCGTCTTCGAACCTCCG16450.10172996599930985No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16370.1012352306023527No Hit
GAATAGGACCGCGGTTCTATTTTGT16220.10030760173305808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA905.3963413E-712.66842116
CGTCTTA1051.9897925E-812.6684215
CGTTATT1457.2759576E-1212.4434532
ATCCCCG1551.8189894E-1212.25938212
GTATTAG2100.012.211821
TAGTACT701.0930111E-412.211824
CGCGGTC1103.8113285E-812.0914610
CGACCAT2050.012.04933110
GCTCGTA1501.4551915E-1112.0342549
CGTCGTA3000.012.03388210
TCTATAG802.8733657E-511.8722353
CCGTCGT3000.011.7175649
GTATCAA22600.011.5153731
TATACCG752.0766123E-411.3984035
ATTTGCG2100.011.311089516
TTTGCGC2100.011.310389517
ACCGTCG3050.011.2139738
GTCGTAG3050.011.21362611
TACACCG855.330531E-511.1749055
AGGCCCG3850.011.10440310
Produced by FastQC (version 0.11.5)