##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064188_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1617026 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25887338855405 32.0 32.0 32.0 32.0 32.0 2 30.896691828084396 32.0 32.0 32.0 32.0 32.0 3 30.909987223458373 32.0 32.0 32.0 32.0 32.0 4 30.932416052679425 32.0 32.0 32.0 32.0 32.0 5 30.857768520728794 32.0 32.0 32.0 32.0 32.0 6 34.49982560577257 36.0 36.0 36.0 32.0 36.0 7 34.44412149217143 36.0 36.0 36.0 32.0 36.0 8 34.41461547309691 36.0 36.0 36.0 32.0 36.0 9 34.5315579341334 36.0 36.0 36.0 32.0 36.0 10 34.268292532092865 36.0 36.0 36.0 32.0 36.0 11 34.49257464011092 36.0 36.0 36.0 32.0 36.0 12 34.33052282399912 36.0 36.0 36.0 32.0 36.0 13 34.40786295334769 36.0 36.0 36.0 32.0 36.0 14 34.30965302969773 36.0 36.0 36.0 32.0 36.0 15 34.25051297876472 36.0 36.0 36.0 32.0 36.0 16 34.248654010510656 36.0 36.0 36.0 32.0 36.0 17 34.18362413467687 36.0 36.0 36.0 32.0 36.0 18 34.170541475523585 36.0 36.0 36.0 32.0 36.0 19 34.19735304194243 36.0 36.0 36.0 32.0 36.0 20 34.17678565465243 36.0 36.0 36.0 32.0 36.0 21 34.16144205473505 36.0 36.0 36.0 32.0 36.0 22 34.133998463846595 36.0 36.0 36.0 32.0 36.0 23 34.09166271909048 36.0 36.0 36.0 32.0 36.0 24 34.07202419750826 36.0 36.0 36.0 32.0 36.0 25 33.68459319763566 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 16.0 5 18.0 6 61.0 7 26.0 8 84.0 9 109.0 10 145.0 11 39.0 12 106.0 13 81.0 14 149.0 15 292.0 16 401.0 17 607.0 18 754.0 19 1030.0 20 1504.0 21 2226.0 22 3550.0 23 5487.0 24 8230.0 25 12298.0 26 17231.0 27 23061.0 28 31501.0 29 42650.0 30 55958.0 31 76187.0 32 107570.0 33 152475.0 34 331670.0 35 741501.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.19078368610488 17.19911311166899 11.65080256712599 25.959300635100142 2 16.781802978989734 19.465664434444662 37.043237653717796 26.709294932847804 3 18.83206957093922 23.323914639297413 28.572532911316472 29.271482878446896 4 12.862935045851586 15.211483800424997 35.27690302024297 36.64867813348044 5 14.968795167533862 36.15214821970725 33.28616479590227 15.592891816856607 6 34.6169641642236 34.88155762546233 16.72400143491545 13.777476775398622 7 30.66474338831831 30.250438551999032 20.476295924249886 18.608522135432775 8 28.45934944260916 32.30720215780162 19.30576692278188 19.927681476807344 9 27.44045497742932 14.1411385325097 18.547494951294844 39.87091153876614 10 16.435125027535015 26.1776942858345 30.86545339342773 26.52172729320275 11 37.02388723714294 21.043353432726843 22.38897121836975 19.543788111760467 12 24.950882627579862 23.405735176454353 28.298351450811154 23.34503074515463 13 29.295723923183836 19.183002127456817 25.307949064797707 26.213324884561644 14 23.91393942244263 19.456700524388324 24.78839439645837 31.840965656710672 15 25.26262899404293 26.84109468504631 22.15619069306085 25.740085627849908 16 25.99748015435911 25.481320220894915 23.37057820308768 25.150621421658297 17 24.182477930647842 25.79722556715729 24.872868403172692 25.14742809902218 18 25.069981117630785 24.605874638644927 25.78145839225693 24.54268585146736 19 25.805726931953 24.63129061744394 25.15461008182127 24.40837236878179 20 26.101013902006386 24.080636835922657 24.560541374901128 25.257807887169825 21 26.99662033997115 23.907644492688956 24.201670583095016 24.89406458424488 22 26.2454828290255 23.859512965572645 24.578980169936607 25.316024035465247 23 24.694268481755756 23.83941474826934 25.27933453610321 26.186982233871692 24 25.02506516944055 24.586084313041066 24.950674222113296 25.43817629540509 25 25.052760960973597 24.206667004996973 25.026891070431706 25.713680963597724 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 172.0 1 172.0 2 355.0 3 538.0 4 538.0 5 538.0 6 1297.0 7 2056.0 8 2056.0 9 2056.0 10 2273.5 11 2491.0 12 2491.0 13 2491.0 14 3097.5 15 3704.0 16 3704.0 17 3704.0 18 6247.0 19 8790.0 20 8790.0 21 8790.0 22 14354.0 23 19918.0 24 19918.0 25 19918.0 26 30153.0 27 40388.0 28 40388.0 29 40388.0 30 51055.5 31 61723.0 32 61723.0 33 61723.0 34 78377.0 35 95031.0 36 95031.0 37 95031.0 38 111002.5 39 126974.0 40 126974.0 41 126974.0 42 147499.0 43 168024.0 44 168024.0 45 168024.0 46 190034.5 47 212045.0 48 212045.0 49 212045.0 50 218539.0 51 225033.0 52 225033.0 53 225033.0 54 211166.5 55 197300.0 56 197300.0 57 197300.0 58 181631.5 59 165963.0 60 165963.0 61 165963.0 62 145241.5 63 124520.0 64 124520.0 65 124520.0 66 101791.5 67 79063.0 68 79063.0 69 79063.0 70 59608.5 71 40154.0 72 40154.0 73 40154.0 74 30438.0 75 20722.0 76 20722.0 77 20722.0 78 16724.5 79 12727.0 80 12727.0 81 12727.0 82 8707.0 83 4687.0 84 4687.0 85 4687.0 86 3675.5 87 2664.0 88 2664.0 89 2664.0 90 1884.0 91 1104.0 92 1104.0 93 1104.0 94 763.0 95 422.0 96 422.0 97 422.0 98 617.5 99 813.0 100 813.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03636305167634905 2 0.006307876311203406 3 0.001793415813969596 4 0.0043289347233748865 5 0.007977608275933721 6 0.012739436471646097 7 0.021149938219917307 8 0.035002529334716946 9 0.047618281957123754 10 0.0557814160069164 11 0.05510115483610034 12 0.0617800826950216 13 0.06258402771507694 14 0.06481033700138403 15 0.05955377340871452 16 0.06518138854910187 17 0.06085245382572699 18 0.07625109305601765 19 0.07340636452351415 20 0.07965239891009793 21 0.07315899682503559 22 0.07711688000069263 23 0.08107476317634967 24 0.07656030267911586 25 0.07717872192531228 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1617026.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.50244626953839 #Duplication Level Percentage of deduplicated Percentage of total 1 77.21256553186208 38.22210876574215 2 12.612838431804182 12.487327135535107 3 3.933968013475284 5.842231206394289 4 1.8028534656081328 3.569826272524707 5 1.0422461534793137 2.579686710612139 6 0.6696546958617765 1.9889727360624998 7 0.4617414756153333 1.6000132810945784 8 0.3421097073650417 1.3548213925700385 9 0.273754075191693 1.219634675841956 >10 1.4532304270241496 12.95636831731002 >50 0.09903322011504577 3.3584279791056946 >100 0.0815350214389226 8.57821140288657 >500 0.01082087982331594 3.6036839413176724 >1k 0.0036489013357693285 2.6386861830026076 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3655 0.22603223448478876 No Hit TATCAACGCAGAGTACTTTTTTTTT 2422 0.14978114142877108 No Hit GCGCAAGACGGACCAGAGCGAAAGC 1857 0.11484045401867378 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 1785 0.11038783544605962 No Hit GTATCTGATCGTCTTCGAACCTCCG 1645 0.10172996599930985 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 1637 0.1012352306023527 No Hit GAATAGGACCGCGGTTCTATTTTGT 1622 0.10030760173305808 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 6.184192461964125E-5 0.0 6.184192461964125E-5 6.184192461964125E-5 6 0.0 6.184192461964125E-5 0.0 6.184192461964125E-5 6.184192461964125E-5 7 0.0 6.184192461964125E-5 0.0 6.184192461964125E-5 6.184192461964125E-5 8 0.0 6.184192461964125E-5 0.0 6.184192461964125E-5 6.184192461964125E-5 9 0.0 6.184192461964125E-5 0.0 6.184192461964125E-5 6.184192461964125E-5 10 0.0 6.184192461964125E-5 0.0 6.184192461964125E-5 6.184192461964125E-5 11 0.0 6.184192461964125E-5 0.0 6.184192461964125E-5 6.184192461964125E-5 12 0.0 6.184192461964125E-5 0.0 6.184192461964125E-5 6.184192461964125E-5 13 0.0 6.184192461964125E-5 0.0 6.184192461964125E-5 1.236838492392825E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGCTA 90 5.3963413E-7 12.668421 16 CGTCTTA 105 1.9897925E-8 12.66842 15 CGTTATT 145 7.2759576E-12 12.443453 2 ATCCCCG 155 1.8189894E-12 12.259382 12 GTATTAG 210 0.0 12.21182 1 TAGTACT 70 1.0930111E-4 12.21182 4 CGCGGTC 110 3.8113285E-8 12.09146 10 CGACCAT 205 0.0 12.049331 10 GCTCGTA 150 1.4551915E-11 12.034254 9 CGTCGTA 300 0.0 12.033882 10 TCTATAG 80 2.8733657E-5 11.872235 3 CCGTCGT 300 0.0 11.717564 9 GTATCAA 2260 0.0 11.515373 1 TATACCG 75 2.0766123E-4 11.398403 5 ATTTGCG 210 0.0 11.3110895 16 TTTGCGC 210 0.0 11.3103895 17 ACCGTCG 305 0.0 11.213973 8 GTCGTAG 305 0.0 11.213626 11 TACACCG 85 5.330531E-5 11.174905 5 AGGCCCG 385 0.0 11.104403 10 >>END_MODULE