FastQCFastQC Report
Thu 2 Feb 2017
SRR4064188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617026
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17730.10964573235062393No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17480.1080996842351329No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16710.10333785603942051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28200.024.497281
GGTATCA13050.020.3991571
CGTCTTA1750.020.11434215
TCAATAC1800.019.5544013
CGGATCG708.123103E-418.85777926
TCTAGAT4050.018.4686162
AACGCAG37600.018.3712546
TCTAGTA1254.8564107E-717.5995042
TAATACT2650.017.4329344
TCAACGC39500.017.3761884
CAACGCA40000.017.1589875
GTCTAGA2450.017.0618021
TATCAAC40500.017.056312
GTCCTAA2201.8189894E-1217.0005741
ATCAACG40500.016.9471453
CGCACGA1053.774523E-516.7624738
CTGTACG800.00198911516.4995379
GTAATAC1351.0866697E-616.2953343
TAGGACG2450.016.1623124
GTGCGTA1105.6229932E-516.00004610
Produced by FastQC (version 0.11.5)