FastQCFastQC Report
Thu 2 Feb 2017
SRR4064187_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064187_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5651
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT390.6901433374623961No Hit
TATCAACGCAGAGTACTTTTTTTTT380.6724473544505397No Hit
GGTATCAACGCAGAGTACTTTTTTT190.33622367722526986No Hit
GTACTTTTTTTTTTTTTTTTTTTTT170.3008317112015573No Hit
GAGTACTTTTTTTTTTTTTTTTTTT150.26543974517784463No Hit
ATATTAAAGTTGCTGCAGTTAAAAA100.1769598301185631No Hit
GGACTCTTTCGAGGCCCTGTAATTG90.15926384710670677No Hit
ACCATACTCCCCCCGGAACCCAAAG90.15926384710670677No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG80.14156786409485048No Hit
CTTTAGGACGTGAAATATGGCGAGG80.14156786409485048No Hit
CTATTGGAGCTGGAATTACCGCGGC70.12387188108299416No Hit
CTCCACTCCTGGTGGTGCCCTTCCG70.12387188108299416No Hit
GTATCTGATCGTCTTCGAACCTCCG60.10617589807113785No Hit
GATTAAGAGGGACGGCCGGGGGCAT60.10617589807113785No Hit
GCGCAAGACGGACCAGAGCGAAAGC60.10617589807113785No Hit
ATCAACGCAGAGTACTTTTTTTTTT60.10617589807113785No Hit
GTCCTAAAGTGTGTATTTCTCATTT60.10617589807113785No Hit
GTCCTATTCCATTATTCCTAGCTGC60.10617589807113785No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT60.10617589807113785No Hit
GTCCTACAGTGTGCATTTCTCATTT60.10617589807113785No Hit
CCTTTAACGAGGATCCATTGGAGGG60.10617589807113785No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)