##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064187_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5651
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.29676163510883	32.0	32.0	32.0	14.0	32.0
2	27.88922314634578	32.0	21.0	32.0	14.0	32.0
3	28.061758980711378	32.0	21.0	32.0	14.0	32.0
4	27.8617943726774	32.0	21.0	32.0	14.0	32.0
5	28.00495487524332	32.0	21.0	32.0	14.0	32.0
6	31.348787825163686	36.0	32.0	36.0	21.0	36.0
7	31.487170412316406	36.0	32.0	36.0	21.0	36.0
8	31.119801804990267	36.0	32.0	36.0	14.0	36.0
9	31.51583790479561	36.0	32.0	36.0	14.0	36.0
10	30.30826402406654	36.0	21.0	36.0	14.0	36.0
11	31.783578127764997	36.0	32.0	36.0	14.0	36.0
12	31.072730490178728	36.0	32.0	36.0	14.0	36.0
13	31.528755972394265	36.0	32.0	36.0	14.0	36.0
14	30.867811006901434	36.0	32.0	36.0	14.0	36.0
15	30.769244381525393	36.0	32.0	36.0	14.0	36.0
16	30.846752787117325	36.0	32.0	36.0	14.0	36.0
17	30.53972748186162	36.0	21.0	36.0	14.0	36.0
18	30.637586267917182	36.0	27.0	36.0	14.0	36.0
19	30.654397451778447	36.0	27.0	36.0	14.0	36.0
20	30.61829764643426	36.0	27.0	36.0	14.0	36.0
21	30.64926561670501	36.0	27.0	36.0	14.0	36.0
22	30.59458502919837	36.0	27.0	36.0	14.0	36.0
23	30.348079985843214	36.0	27.0	36.0	14.0	36.0
24	30.489470890107945	36.0	27.0	36.0	14.0	36.0
25	29.404353211820915	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	12.0
16	24.0
17	48.0
18	71.0
19	103.0
20	133.0
21	147.0
22	146.0
23	166.0
24	166.0
25	183.0
26	188.0
27	204.0
28	224.0
29	259.0
30	322.0
31	360.0
32	465.0
33	520.0
34	829.0
35	1074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.511771995043375	19.560984244999116	12.426978226234732	26.500265533722782
2	15.377809237303133	21.075915767120865	39.07273049017873	24.473544505397275
3	18.244558485223855	24.385064590337993	29.269155901610333	28.101221022827815
4	11.183861263493187	16.77579189523978	35.94054149708016	36.09980534418687
5	14.634577950805166	37.16156432489825	33.852415501681115	14.351442222615466
6	31.19801804990267	36.80764466466112	17.288975402583613	14.705361882852591
7	28.331268801981953	30.826402406653692	21.85453901964254	18.98778977172182
8	26.543974517784463	33.6577596885507	19.890284905326492	19.907980888338347
9	25.871527163333923	13.466643072022652	20.297292514599185	40.36453725004424
10	16.01769911504425	27.398230088495573	31.840707964601773	24.743362831858406
11	37.26774022296939	21.146699699168288	23.464873473721465	18.12068660414086
12	24.853956452469465	23.915737298636927	29.598158966188702	21.632147282704903
13	30.106194690265486	19.787610619469024	25.76991150442478	24.336283185840706
14	22.58007432312865	20.173420633516194	26.933286144045304	30.313218899309856
15	23.575221238938056	28.61946902654867	22.707964601769913	25.097345132743364
16	24.495575221238937	26.10619469026549	24.265486725663717	25.13274336283186
17	21.889930985666254	26.437798619713327	26.73863033091488	24.93364006370554
18	22.41898353107845	25.110678236231625	29.077386222773153	23.39295200991677
19	25.21246458923513	24.539660056657222	26.363314447592067	23.88456090651558
20	25.221395678356355	24.79631597591215	26.620616365568544	23.36167198016295
21	24.68589630153955	24.668200318527695	25.92461511236949	24.721288267563263
22	24.20325779036827	25.63739376770538	26.363314447592067	23.79603399433428
23	22.972015586255758	25.07970244420829	27.78958554729012	24.158696422245836
24	23.79182156133829	24.092759780492123	26.907417241989734	25.208001416179854
25	23.371104815864022	25.01770538243626	27.638101983002834	23.973087818696882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	3.5
3	6.0
4	6.0
5	6.0
6	13.5
7	21.0
8	21.0
9	21.0
10	25.0
11	29.0
12	29.0
13	29.0
14	21.5
15	14.0
16	14.0
17	14.0
18	31.0
19	48.0
20	48.0
21	48.0
22	84.5
23	121.0
24	121.0
25	121.0
26	171.5
27	222.0
28	222.0
29	222.0
30	284.5
31	347.0
32	347.0
33	347.0
34	372.0
35	397.0
36	397.0
37	397.0
38	436.5
39	476.0
40	476.0
41	476.0
42	551.5
43	627.0
44	627.0
45	627.0
46	655.5
47	684.0
48	684.0
49	684.0
50	698.0
51	712.0
52	712.0
53	712.0
54	656.0
55	600.0
56	600.0
57	600.0
58	548.0
59	496.0
60	496.0
61	496.0
62	426.5
63	357.0
64	357.0
65	357.0
66	297.0
67	237.0
68	237.0
69	237.0
70	175.0
71	113.0
72	113.0
73	113.0
74	87.0
75	61.0
76	61.0
77	61.0
78	49.0
79	37.0
80	37.0
81	37.0
82	30.0
83	23.0
84	23.0
85	23.0
86	15.5
87	8.0
88	8.0
89	8.0
90	7.0
91	6.0
92	6.0
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	4.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03539196602371262
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.01769598301185631
11	0.0
12	0.03539196602371262
13	0.01769598301185631
14	0.0
15	0.01769598301185631
16	0.01769598301185631
17	0.0
18	0.07078393204742524
19	0.053087949035568925
20	0.08847991505928154
21	0.0
22	0.053087949035568925
23	0.08847991505928154
24	0.03539196602371262
25	0.053087949035568925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5651.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.20545036276765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.18766117833763	84.02052734029375
2	3.8682801031541363	6.901433374623961
3	0.9323546915294585	2.4951336046717394
4	0.35707200952192025	1.2741107768536541
5	0.23804800634794684	1.0617589807113785
6	0.17853600476096013	0.9555830826402406
7	0.0396746677246578	0.2477437621659883
8	0.0396746677246578	0.28313572818970095
9	0.0396746677246578	0.31852769421341354
>10	0.11902400317397342	2.4420456556361705
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	39	0.6901433374623961	No Hit
TATCAACGCAGAGTACTTTTTTTTT	38	0.6724473544505397	No Hit
GGTATCAACGCAGAGTACTTTTTTT	19	0.33622367722526986	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	17	0.3008317112015573	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	15	0.26543974517784463	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	10	0.1769598301185631	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	9	0.15926384710670677	No Hit
ACCATACTCCCCCCGGAACCCAAAG	9	0.15926384710670677	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	8	0.14156786409485048	No Hit
CTTTAGGACGTGAAATATGGCGAGG	8	0.14156786409485048	No Hit
CTATTGGAGCTGGAATTACCGCGGC	7	0.12387188108299416	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	7	0.12387188108299416	No Hit
GTATCTGATCGTCTTCGAACCTCCG	6	0.10617589807113785	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	6	0.10617589807113785	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	6	0.10617589807113785	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	6	0.10617589807113785	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6	0.10617589807113785	No Hit
GTCCTATTCCATTATTCCTAGCTGC	6	0.10617589807113785	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	6	0.10617589807113785	No Hit
GTCCTACAGTGTGCATTTCTCATTT	6	0.10617589807113785	No Hit
CCTTTAACGAGGATCCATTGGAGGG	6	0.10617589807113785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE