##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064187_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5651
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.95717572111131	32.0	32.0	32.0	21.0	32.0
2	29.838435675101753	32.0	32.0	32.0	21.0	32.0
3	30.800920191116617	32.0	32.0	32.0	32.0	32.0
4	31.043709078039285	32.0	32.0	32.0	32.0	32.0
5	30.87453548044594	32.0	32.0	32.0	32.0	32.0
6	34.42665015041585	36.0	36.0	36.0	32.0	36.0
7	34.49654928331269	36.0	36.0	36.0	32.0	36.0
8	34.21677579189524	36.0	36.0	36.0	32.0	36.0
9	34.625022119978766	36.0	36.0	36.0	32.0	36.0
10	34.28809060343302	36.0	36.0	36.0	32.0	36.0
11	34.713325075207926	36.0	36.0	36.0	32.0	36.0
12	34.61971332507521	36.0	36.0	36.0	32.0	36.0
13	34.59918598478146	36.0	36.0	36.0	32.0	36.0
14	34.45797204034684	36.0	36.0	36.0	32.0	36.0
15	34.39992921606795	36.0	36.0	36.0	32.0	36.0
16	34.380817554415145	36.0	36.0	36.0	32.0	36.0
17	34.37055388426827	36.0	36.0	36.0	32.0	36.0
18	34.42576535126526	36.0	36.0	36.0	32.0	36.0
19	34.41196248451602	36.0	36.0	36.0	32.0	36.0
20	34.47301362590692	36.0	36.0	36.0	32.0	36.0
21	34.50911343125111	36.0	36.0	36.0	32.0	36.0
22	34.43496726243143	36.0	36.0	36.0	32.0	36.0
23	34.300300831711205	36.0	36.0	36.0	32.0	36.0
24	34.22845514068307	36.0	36.0	36.0	32.0	36.0
25	34.43213590514953	36.0	36.0	36.0	32.0	36.0
26	34.19943372854362	36.0	36.0	36.0	32.0	36.0
27	34.11449301008671	36.0	36.0	36.0	32.0	36.0
28	34.10688373739161	36.0	36.0	36.0	32.0	36.0
29	34.11608564855778	36.0	36.0	36.0	32.0	36.0
30	34.153247212882675	36.0	36.0	36.0	32.0	36.0
31	33.97186338701115	36.0	36.0	36.0	32.0	36.0
32	33.960007078393204	36.0	36.0	36.0	32.0	36.0
33	33.84250575119448	36.0	36.0	36.0	32.0	36.0
34	34.042824278888695	36.0	36.0	36.0	32.0	36.0
35	33.777561493540965	36.0	36.0	36.0	32.0	36.0
36	33.801628030437094	36.0	36.0	36.0	32.0	36.0
37	33.6549283312688	36.0	36.0	36.0	27.0	36.0
38	33.64147938417979	36.0	36.0	36.0	27.0	36.0
39	33.697752610157494	36.0	36.0	36.0	27.0	36.0
40	33.808529463811716	36.0	36.0	36.0	32.0	36.0
41	33.76340470713148	36.0	36.0	36.0	32.0	36.0
42	33.493187046540434	36.0	36.0	36.0	21.0	36.0
43	33.630684834542556	36.0	36.0	36.0	27.0	36.0
44	33.38170235356574	36.0	36.0	36.0	21.0	36.0
45	33.44381525393736	36.0	36.0	36.0	21.0	36.0
46	33.28809060343302	36.0	36.0	36.0	21.0	36.0
47	33.210759157671205	36.0	36.0	36.0	21.0	36.0
48	33.28048133073792	36.0	36.0	36.0	21.0	36.0
49	33.32188993098567	36.0	36.0	36.0	21.0	36.0
50	32.31799681472306	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	1.0
24	11.0
25	14.0
26	31.0
27	60.0
28	97.0
29	172.0
30	254.0
31	413.0
32	555.0
33	810.0
34	1240.0
35	1992.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.74482392496903	19.46558131304194	13.289683241904088	25.49991152008494
2	16.280304370907803	21.90762696867811	37.3031321889931	24.508936471420988
3	21.730667138559546	24.15501681118386	29.23376393558662	24.88055211466997
4	14.139090426473192	17.324367368607327	37.48009201911166	31.056450185807822
5	18.12068660414086	34.31251105998938	32.17129711555477	15.39550522031499
6	34.436382941072374	36.913820562732255	15.76712086356397	12.882675632631393
7	30.401698814369137	30.11856308617944	20.226508582551762	19.253229516899665
8	26.420102636701472	34.47177490709608	19.69562909219607	19.41249336400637
9	26.88019819500973	13.590514953105645	18.84622190762697	40.68306494425765
10	15.997168642718105	26.632454432843744	31.81737745531764	25.552999469120508
11	36.77225269863741	20.98743585206158	23.128649796496195	19.111661652804813
12	25.26986374093081	23.836489116970448	27.747301362590694	23.146345779508053
13	28.844452309325785	19.624845160148645	25.057511944788537	26.47319058573704
14	23.11095381348434	20.562732259777032	25.145991859847815	31.180322066890813
15	25.022119978764824	28.66749247920722	21.90762696867811	24.40276057334985
16	24.969032029729252	25.960007078393204	24.36736860732614	24.703592284551405
17	21.76605910458326	26.526278534772608	26.190054857547334	25.517607503096794
18	24.615112369492127	25.110599893824105	27.110245974163867	23.164041762519908
19	25.429127588037513	24.455848522385416	25.78304724827464	24.331976641302425
20	25.977703061405062	25.12829587683596	24.06653689612458	24.8274641656344
21	25.535303486108653	26.136966908511766	24.420456556361707	23.907273049017874
22	24.809768182622545	25.960007078393204	24.73898425057512	24.49124048840913
23	22.77473013625907	26.526278534772608	25.853831180322068	24.845160148646258
24	24.314280658290567	25.94231109538135	26.190054857547334	23.553353388780746
25	24.06653689612458	25.694567333215364	25.836135197310213	24.40276057334985
26	23.411785524685897	26.06618297646434	26.59706246682003	23.92496903202973
27	24.331976641302425	25.88922314634578	25.58839143514422	24.190408777207573
28	23.411785524685897	26.844806228986016	25.48221553707308	24.261192709255
29	23.659529286851885	25.88922314634578	25.287559723942664	25.163687842859673
30	23.53565740576889	25.075207927800392	25.641479384179792	25.747655282250932
31	24.915944080693684	26.013095027428772	24.43815253937356	24.632808352503982
32	23.659529286851885	26.190054857547334	26.136966908511766	24.01344894708901
33	23.871881082994157	25.517607503096794	25.376039639001945	25.234471774907096
34	24.93364006370554	24.562024420456556	26.27853477260662	24.22580074323129
35	24.77437621659883	26.190054857547334	25.305255706954522	23.73031321889931
36	24.048840913112723	25.729959299239074	25.022119978764824	25.199079808883383
37	24.526632454432846	27.517253583436556	24.668200318527695	23.287913643602902
38	23.64183330384003	25.94231109538135	25.322951689966377	25.092903910812247
39	24.579720403468414	26.190054857547334	24.845160148646258	24.385064590337993
40	24.8274641656344	25.836135197310213	25.818439214298355	23.517961422757033
41	23.35869757565033	25.78304724827464	27.18102990621129	23.67722526986374
42	25.03981596177668	27.18102990621129	25.057511944788537	22.7216421872235
43	23.323305609626615	27.0571580251283	27.12794195717572	22.491594408069368
44	23.624137320828172	27.021766059104586	25.429127588037513	23.92496903202973
45	23.6949212528756	27.464165634400988	24.455848522385416	24.385064590337993
46	23.78340116793488	27.03946204211644	25.464519554061226	23.712617235887453
47	24.137320828172005	26.561670500796318	26.136966908511766	23.164041762519908
48	24.845160148646258	26.667846398867457	24.172712794195718	24.314280658290567
49	24.06653689612458	26.61475844983189	24.420456556361707	24.898248097681826
50	24.5443284374447	26.349318704654046	24.668200318527695	24.43815253937356
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	1.0
4	0.0
5	0.5
6	1.0
7	2.0
8	3.0
9	4.5
10	6.0
11	7.0
12	8.0
13	6.0
14	4.0
15	9.0
16	14.0
17	16.0
18	18.0
19	24.0
20	30.0
21	27.5
22	25.0
23	36.0
24	47.0
25	49.0
26	51.0
27	69.0
28	87.0
29	100.5
30	114.0
31	130.5
32	147.0
33	158.5
34	170.0
35	196.0
36	222.0
37	248.5
38	275.0
39	257.5
40	240.0
41	263.5
42	287.0
43	289.0
44	291.0
45	288.0
46	285.0
47	324.0
48	363.0
49	368.0
50	373.0
51	377.5
52	382.0
53	375.5
54	369.0
55	379.5
56	390.0
57	374.0
58	358.0
59	326.5
60	295.0
61	250.5
62	206.0
63	180.0
64	154.0
65	143.5
66	133.0
67	108.0
68	83.0
69	75.5
70	68.0
71	55.5
72	43.0
73	39.5
74	36.0
75	28.5
76	21.0
77	17.0
78	13.0
79	10.5
80	8.0
81	8.5
82	9.0
83	7.5
84	6.0
85	6.0
86	6.0
87	3.0
88	0.0
89	1.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5651.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.48787825163689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.79784213515047	89.55937002300477
2	2.7635812985046373	5.167227039462042
3	0.7950028392958546	2.229693859493895
4	0.3028582244936589	1.1325429127588038
5	0.18928639030853683	0.8847991505928154
6	0.01892863903085368	0.10617589807113785
7	0.09464319515426842	0.6193594054149708
8	0.01892863903085368	0.14156786409485048
9	0.01892863903085368	0.15926384710670677
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	9	0.15926384710670677	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	8	0.14156786409485048	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	7	0.12387188108299416	No Hit
GAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCG	7	0.12387188108299416	No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC	7	0.12387188108299416	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	7	0.12387188108299416	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	7	0.12387188108299416	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG	6	0.10617589807113785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.01769598301185631	0.0
30	0.0	0.0	0.0	0.01769598301185631	0.0
31	0.0	0.0	0.0	0.03539196602371262	0.0
32	0.0	0.0	0.0	0.07078393204742524	0.0
33	0.0	0.0	0.0	0.08847991505928154	0.0
34	0.0	0.0	0.0	0.10617589807113785	0.0
35	0.0	0.0	0.0	0.14156786409485048	0.0
36	0.0	0.0	0.0	0.1769598301185631	0.0
37	0.0	0.0	0.0	0.1946558131304194	0.0
38	0.0	0.0	0.0	0.2477437621659883	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE