FastQCFastQC Report
Thu 2 Feb 2017
SRR4064186_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064186_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715041
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27290.3816564364840618No Hit
TATCAACGCAGAGTACTTTTTTTTT20700.2894938891616005No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19770.2764876419673837No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12070.168801509284083No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11990.1676826923211396No Hit
GTACATGGGGTGGTATCAACGCAAA10190.14250931065491348No Hit
GGTATCAACGCAGAGTACTTTTTTT9210.12880380285885704No Hit
GTATCAACGCAGAGTACATGGGGTG7750.1083853932851403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAGT603.7071004E-919.0009118
CGCATCG755.8216756E-815.20179213
CATCGCC755.8216756E-815.20179215
TCGCACG456.7535555E-414.77848518
GCGTTAT456.7607267E-414.7764171
CGTTATT400.005283538714.2466952
AACCGCG551.9570251E-413.8188447
GCGTCCC655.4384294E-513.15539716
TCGCCAG905.383572E-712.668159517
ATCGCCA905.383572E-712.668159516
CGGGAGT751.4783996E-512.6646154
CCGCATC1001.4359648E-712.35145612
CCGTCGT853.9327897E-612.2947069
CGTCGTA853.9327897E-612.29470610
CGCACGC550.003064557712.09148819
GCATCGC951.0356762E-612.00141414
AGGCCCG1209.966243E-911.87556810
ATATTAT658.0203085E-411.6912321
TATAAGA658.029731E-411.6895952
ACCGTCG907.440716E-611.6116678
Produced by FastQC (version 0.11.5)