##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064186_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 715041 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.158842639792685 32.0 32.0 32.0 32.0 32.0 2 30.754745532074384 32.0 32.0 32.0 32.0 32.0 3 30.779456003222194 32.0 32.0 32.0 32.0 32.0 4 30.79199514433438 32.0 32.0 32.0 32.0 32.0 5 30.71154101652912 32.0 32.0 32.0 32.0 32.0 6 34.34086716705755 36.0 36.0 36.0 32.0 36.0 7 34.27011178379981 36.0 36.0 36.0 32.0 36.0 8 34.207224480833965 36.0 36.0 36.0 32.0 36.0 9 34.35362168043511 36.0 36.0 36.0 32.0 36.0 10 34.04960135153089 36.0 36.0 36.0 32.0 36.0 11 34.36624333429831 36.0 36.0 36.0 32.0 36.0 12 34.15164724819975 36.0 36.0 36.0 32.0 36.0 13 34.24620686086532 36.0 36.0 36.0 32.0 36.0 14 34.148230660899166 36.0 36.0 36.0 32.0 36.0 15 34.07934789753315 36.0 36.0 36.0 32.0 36.0 16 34.07983178586962 36.0 36.0 36.0 32.0 36.0 17 34.00408368191474 36.0 36.0 36.0 32.0 36.0 18 33.99116694007756 36.0 36.0 36.0 32.0 36.0 19 34.022226697490076 36.0 36.0 36.0 32.0 36.0 20 33.97641114285754 36.0 36.0 36.0 32.0 36.0 21 33.95275515669731 36.0 36.0 36.0 32.0 36.0 22 33.946899548417505 36.0 36.0 36.0 32.0 36.0 23 33.89692339320403 36.0 36.0 36.0 32.0 36.0 24 33.90379013231409 36.0 36.0 36.0 32.0 36.0 25 33.50137544560382 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 5.0 5 14.0 6 32.0 7 13.0 8 33.0 9 49.0 10 51.0 11 16.0 12 38.0 13 37.0 14 103.0 15 157.0 16 231.0 17 290.0 18 367.0 19 534.0 20 815.0 21 1168.0 22 1839.0 23 2936.0 24 4258.0 25 6215.0 26 8784.0 27 11475.0 28 15748.0 29 21275.0 30 27454.0 31 36762.0 32 51088.0 33 71823.0 34 148269.0 35 303160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.02258138532741 17.565631523278135 11.871456793163466 25.540330298230984 2 16.241512178406843 20.358603906321022 37.90669864824229 25.493185267029837 3 18.92359460049117 24.66364953540281 28.68726819089602 27.725487673209997 4 12.546817527027592 15.973203171983608 36.137536537950524 35.34244276303828 5 14.3616820403796 37.07524144538544 33.41254467001881 15.15053184421615 6 33.01759588217193 35.93224606260665 17.474053766749655 13.576104288471761 7 29.276586815876904 31.094946668998073 21.19265605875153 18.435810456373492 8 27.805881908556497 32.8752577636884 19.91446582410234 19.40439450365276 9 27.571259871164166 14.326130391712418 19.124173788456265 38.97843594866715 10 15.953235522532166 27.135011089422022 31.797885663511256 25.11386772453456 11 36.18395829077242 21.587407753595407 22.96078425885244 19.267849696779738 12 24.763997145296035 24.03674731671821 29.178572927890738 22.020682610095015 13 29.46237401796285 20.051470361859874 25.51547329502677 24.970682325150506 14 23.42611886306356 20.009460009767878 26.11172607394201 30.45269505322655 15 24.788909381463206 27.452135534590315 23.169311887963147 24.58964319598333 16 24.929678775445087 26.096521303482618 24.530422151255706 24.44337776981659 17 23.349090267923863 26.16140922410391 25.817723332857547 24.671777175114677 18 23.966931738980747 25.45114076263572 27.032197748984636 23.5497297493989 19 25.006717646136796 25.07137499020343 26.335132169687743 23.58677519397203 20 25.517287710498028 24.67263637585095 25.700493774632747 24.109582139018272 21 26.54854871665687 24.41486830688275 25.14820723822319 23.888375738237187 22 25.427628540478807 24.748293264588074 25.59277391039044 24.231304284542674 23 24.191309021285175 24.643660915228807 26.07853455901289 25.086495504473135 24 24.18840165930504 25.296144523005783 26.173381701739356 24.34207211594982 25 24.4380375224806 24.942022799000707 26.212412789273692 24.407526889245002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 585.0 1 585.0 2 650.5 3 716.0 4 716.0 5 716.0 6 1642.5 7 2569.0 8 2569.0 9 2569.0 10 2456.5 11 2344.0 12 2344.0 13 2344.0 14 2256.5 15 2169.0 16 2169.0 17 2169.0 18 3748.0 19 5327.0 20 5327.0 21 5327.0 22 8608.0 23 11889.0 24 11889.0 25 11889.0 26 17297.5 27 22706.0 28 22706.0 29 22706.0 30 28422.5 31 34139.0 32 34139.0 33 34139.0 34 40649.5 35 47160.0 36 47160.0 37 47160.0 38 53918.0 39 60676.0 40 60676.0 41 60676.0 42 68314.0 43 75952.0 44 75952.0 45 75952.0 46 84819.5 47 93687.0 48 93687.0 49 93687.0 50 95881.0 51 98075.0 52 98075.0 53 98075.0 54 89325.5 55 80576.0 56 80576.0 57 80576.0 58 73395.5 59 66215.0 60 66215.0 61 66215.0 62 57263.0 63 48311.0 64 48311.0 65 48311.0 66 39320.0 67 30329.0 68 30329.0 69 30329.0 70 22895.0 71 15461.0 72 15461.0 73 15461.0 74 11618.5 75 7776.0 76 7776.0 77 7776.0 78 6258.0 79 4740.0 80 4740.0 81 4740.0 82 3227.5 83 1715.0 84 1715.0 85 1715.0 86 1354.5 87 994.0 88 994.0 89 994.0 90 704.0 91 414.0 92 414.0 93 414.0 94 288.0 95 162.0 96 162.0 97 162.0 98 258.0 99 354.0 100 354.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040976671267801426 2 0.006433197536924456 3 0.0018180775647829983 4 0.004335415731405612 5 0.010628761147962145 6 0.014125064157160219 7 0.02321545198107521 8 0.03342465676793359 9 0.046570756082518344 10 0.05538143966569749 11 0.0530039536194428 12 0.0602762638785748 13 0.06363271476740494 14 0.06349286264703702 15 0.05859803843415972 16 0.06475153173034832 17 0.06083567236004649 18 0.07258325047095202 19 0.0706253207858011 20 0.07621940560051801 21 0.0700659123043294 22 0.07258325047095202 23 0.0773382225634614 24 0.07342236319315955 25 0.07496073651720671 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 715041.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.05919943361666 #Duplication Level Percentage of deduplicated Percentage of total 1 79.49511584237436 48.53908132217995 2 13.12708011839361 16.030580058601192 3 3.5923541759859985 6.580388102031442 4 1.4645038544795284 3.576857316878633 5 0.6631326079950441 2.024517308125187 6 0.3843994379852739 1.40826731676678 7 0.24089146827687502 1.029604814236212 8 0.1764137235691618 0.8617344584189095 9 0.1155007811632564 0.6347146708607209 >10 0.6021768939112947 6.923995784871428 >50 0.07439828602310798 3.229812838099038 >100 0.06057780211751511 6.964774269365881 >500 0.0020730046349754267 0.7610030606446261 >1k 0.0013820030899836177 1.4346686789199465 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2729 0.3816564364840618 No Hit TATCAACGCAGAGTACTTTTTTTTT 2070 0.2894938891616005 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1977 0.2764876419673837 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1207 0.168801509284083 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1199 0.1676826923211396 No Hit GTACATGGGGTGGTATCAACGCAAA 1019 0.14250931065491348 No Hit GGTATCAACGCAGAGTACTTTTTTT 921 0.12880380285885704 No Hit GTATCAACGCAGAGTACATGGGGTG 775 0.1083853932851403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.3985212036792295E-4 13 0.0 0.0 0.0 0.0 1.3985212036792295E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCAGT 60 3.7071004E-9 19.00091 18 CGCATCG 75 5.8216756E-8 15.201792 13 CATCGCC 75 5.8216756E-8 15.201792 15 TCGCACG 45 6.7535555E-4 14.778485 18 GCGTTAT 45 6.7607267E-4 14.776417 1 CGTTATT 40 0.0052835387 14.246695 2 AACCGCG 55 1.9570251E-4 13.818844 7 GCGTCCC 65 5.4384294E-5 13.155397 16 TCGCCAG 90 5.383572E-7 12.6681595 17 ATCGCCA 90 5.383572E-7 12.6681595 16 CGGGAGT 75 1.4783996E-5 12.664615 4 CCGCATC 100 1.4359648E-7 12.351456 12 CCGTCGT 85 3.9327897E-6 12.294706 9 CGTCGTA 85 3.9327897E-6 12.294706 10 CGCACGC 55 0.0030645577 12.091488 19 GCATCGC 95 1.0356762E-6 12.001414 14 AGGCCCG 120 9.966243E-9 11.875568 10 ATATTAT 65 8.0203085E-4 11.691232 1 TATAAGA 65 8.029731E-4 11.689595 2 ACCGTCG 90 7.440716E-6 11.611667 8 >>END_MODULE