FastQCFastQC Report
Thu 2 Feb 2017
SRR4064186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715041
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9910.13859345128461167No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7570.10586805511851767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT508.342523E-526.3973126
ATAGCGT1351.1277734E-1021.1830316
AGCGTAT1401.8553692E-1020.4264958
TAGCGTA1401.8553692E-1020.4264957
CGAGACT1301.6207196E-920.30846620
CGTCGTA1201.4306352E-820.16461410
TTAACGG550.00447904720.00216335
TATGCCG550.004484489619.9979675
CGACGGT1705.456968E-1219.409797
ACGACGG1705.456968E-1219.409796
ACTTAGG808.984223E-519.2493881
CGTATAT1504.656613E-1019.06472610
CCGTCCA1051.792343E-618.8552259
TACGACG1658.185452E-1118.6647685
CCGTCGT1303.58541E-818.6134919
CTAATAC1901.8189894E-1218.5244313
AATAGCG1557.203198E-1018.4497385
TTGCGGT600.00741356418.33146911
ACGAGAC1456.3027983E-918.20504419
ACGAACG1456.3027983E-918.20504415
Produced by FastQC (version 0.11.5)