##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	715041
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263898154091862	32.0	32.0	32.0	32.0	32.0
2	31.394563388672818	32.0	32.0	32.0	32.0	32.0
3	31.46224342380367	32.0	32.0	32.0	32.0	32.0
4	31.54277027471152	32.0	32.0	32.0	32.0	32.0
5	31.490381670421694	32.0	32.0	32.0	32.0	32.0
6	35.054910138020055	36.0	36.0	36.0	36.0	36.0
7	35.073492289253345	36.0	36.0	36.0	36.0	36.0
8	35.02728934424739	36.0	36.0	36.0	36.0	36.0
9	35.135325107231616	36.0	36.0	36.0	36.0	36.0
10	34.985953253030246	36.0	36.0	36.0	36.0	36.0
11	35.13079389853169	36.0	36.0	36.0	36.0	36.0
12	35.04560997201559	36.0	36.0	36.0	36.0	36.0
13	35.07106585496496	36.0	36.0	36.0	36.0	36.0
14	35.02693132281925	36.0	36.0	36.0	36.0	36.0
15	34.9995063220151	36.0	36.0	36.0	36.0	36.0
16	35.004134028678074	36.0	36.0	36.0	36.0	36.0
17	34.975660136971165	36.0	36.0	36.0	36.0	36.0
18	34.96439225163312	36.0	36.0	36.0	36.0	36.0
19	34.96165814267993	36.0	36.0	36.0	36.0	36.0
20	34.95547807748087	36.0	36.0	36.0	36.0	36.0
21	34.95134265028159	36.0	36.0	36.0	36.0	36.0
22	34.934363204347726	36.0	36.0	36.0	36.0	36.0
23	34.88258715234511	36.0	36.0	36.0	32.0	36.0
24	34.864283306831354	36.0	36.0	36.0	32.0	36.0
25	34.832710292137094	36.0	36.0	36.0	32.0	36.0
26	34.76968453557209	36.0	36.0	36.0	32.0	36.0
27	34.77051805420948	36.0	36.0	36.0	32.0	36.0
28	34.74702429650887	36.0	36.0	36.0	32.0	36.0
29	34.70721259340373	36.0	36.0	36.0	32.0	36.0
30	34.7007388387519	36.0	36.0	36.0	32.0	36.0
31	34.677005933925464	36.0	36.0	36.0	32.0	36.0
32	34.628537384569555	36.0	36.0	36.0	32.0	36.0
33	34.591258403364286	36.0	36.0	36.0	32.0	36.0
34	34.578225584267194	36.0	36.0	36.0	32.0	36.0
35	34.523603541615095	36.0	36.0	36.0	32.0	36.0
36	34.49675892711047	36.0	36.0	36.0	32.0	36.0
37	34.48521972865892	36.0	36.0	36.0	32.0	36.0
38	34.43603094088311	36.0	36.0	36.0	32.0	36.0
39	34.43204375693142	36.0	36.0	36.0	32.0	36.0
40	34.398199543802384	36.0	36.0	36.0	32.0	36.0
41	34.36845299780013	36.0	36.0	36.0	32.0	36.0
42	34.31273731156675	36.0	36.0	36.0	32.0	36.0
43	34.28762406631228	36.0	36.0	36.0	32.0	36.0
44	34.21246194274175	36.0	36.0	36.0	32.0	36.0
45	34.160622957284964	36.0	36.0	36.0	32.0	36.0
46	34.13313782006906	36.0	36.0	36.0	32.0	36.0
47	34.078492002556494	36.0	36.0	36.0	32.0	36.0
48	34.007119871447934	36.0	36.0	36.0	32.0	36.0
49	33.98295482356956	36.0	36.0	36.0	32.0	36.0
50	33.40663402518177	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	6.0
21	29.0
22	100.0
23	252.0
24	680.0
25	1603.0
26	3073.0
27	5626.0
28	9167.0
29	13844.0
30	19817.0
31	27399.0
32	38970.0
33	61006.0
34	122236.0
35	411229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26568575344765	18.524294385856948	12.370415955691067	25.83960390500434
2	15.577183150987436	21.171610261344583	37.46428486517405	25.78692172249393
3	18.068715325220932	25.365820230281788	29.144505808790317	27.420958635706967
4	12.212726263249241	16.679742839921065	36.22337740073646	34.88415349609323
5	13.891790820386523	37.421630368048824	33.3338927418148	15.352686069749847
6	33.0128846118883	36.23242928008123	17.201019804514157	13.553666303516312
7	29.136551801297827	31.105952114567014	21.401180353546657	18.3563157305885
8	27.788336612865557	33.00831700559828	19.795927785959126	19.407418595577035
9	27.62759864901718	14.15972644695714	18.954596628137086	39.25807827588859
10	15.98660766730626	27.30435950078038	31.80776911931707	24.90126371259629
11	36.365467155030274	21.56435784801151	23.022176350726742	19.047998646231477
12	24.741761976286575	24.175078807000467	29.12530522853331	21.95785398817965
13	29.6104956806431	20.078541390165825	25.56035561796103	24.75060731123005
14	23.61829214909956	19.890103896430826	26.18500529341756	30.306598661052053
15	24.78304833004905	27.481570471356786	23.177221368323448	24.55815983027072
16	24.98360224715644	26.005507407308993	24.681799683093324	24.329090662441246
17	23.41404199199766	26.071092426867832	25.95851146997165	24.556354111162857
18	24.064874873782934	25.301872068337627	27.221909884509632	23.411343173369804
19	25.245825064576717	25.046395940932058	26.395129789760308	23.312649204730917
20	25.711676458700737	24.43789622075501	25.975439033224855	23.874988287319397
21	26.611061463608383	24.297347984241462	25.42819222953649	23.66339832261367
22	25.496930936634225	24.520340848541128	25.866421272126416	24.11630694269823
23	24.323352908187452	24.425166390921817	26.388572275685178	24.86290842520555
24	24.38971860276153	25.093596293349034	26.312550783943255	24.204134319946185
25	24.575224642495016	24.780112583476104	26.320897870703817	24.32376490332506
26	24.157072368421055	25.514539697457934	26.55984268707483	23.768545247046184
27	24.541744406246764	25.105665222365502	26.125114336623806	24.227476034763928
28	23.98976796096273	25.11744607410536	26.574490670746805	24.31829529418511
29	24.18543894760544	25.1164657623525	26.28147302791402	24.416622262128033
30	24.16536249092671	25.345344337359844	26.431061899747	24.058231271966445
31	24.545029874460134	25.15230939646877	25.681825810638443	24.620834918432656
32	24.20179326414073	25.599677766293848	25.51953891489478	24.678990054670642
33	24.0893420325732	25.09325109614618	25.99464339410214	24.82276347717848
34	24.639519564542432	25.199785180233224	26.146334777586016	24.014360477638323
35	25.045488556184136	25.098914008797017	26.05511772480298	23.800479710215868
36	24.219537813656917	25.7026292565102	25.758853583181935	24.318979346650945
37	24.91528149759096	25.531492346314412	25.58911351510108	23.964112640993545
38	24.302849582227246	25.506795975283293	25.892819679376544	24.297534763112917
39	24.616821933211845	25.444086561390307	25.526183659740393	24.412907845657454
40	25.071921165731258	25.45666618649295	25.796377438921205	23.675035208854588
41	24.128517224066	25.73197560337726	26.297136991148463	23.842370181408274
42	24.909653887679923	25.990730432934093	25.63619886214246	23.46341681724352
43	24.186228646150187	25.398337144335592	25.816638346374653	24.598795863139568
44	24.043153373769808	25.877479437954438	25.404073389825054	24.675293798450696
45	24.25860065199788	25.907195891658148	25.43518970453112	24.399013751812852
46	24.247764095463857	25.647835420643744	25.337221254903607	24.76717922898879
47	24.449737210883022	25.725362296544724	25.56298967024791	24.26191082232435
48	24.813539031284044	25.981743397820228	24.928499403520696	24.27621816737503
49	24.218153440796154	26.08840369323029	25.03601207208568	24.657430793887876
50	24.27174282441691	26.41878860857392	24.975070941178608	24.33439762583056
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	293.0
1	166.0
2	39.0
3	134.0
4	229.0
5	237.5
6	246.0
7	254.0
8	262.0
9	335.5
10	409.0
11	603.0
12	797.0
13	1480.5
14	2164.0
15	2786.0
16	3408.0
17	3583.0
18	3758.0
19	3702.5
20	3647.0
21	3868.0
22	4089.0
23	4466.5
24	4844.0
25	5069.5
26	5295.0
27	6223.5
28	7152.0
29	8448.5
30	9745.0
31	11353.5
32	12962.0
33	15135.0
34	17308.0
35	19912.5
36	22517.0
37	25404.0
38	28291.0
39	30293.5
40	32296.0
41	34775.0
42	37254.0
43	38455.0
44	39656.0
45	43315.0
46	46974.0
47	49465.0
48	51956.0
49	53319.0
50	54682.0
51	53346.5
52	52011.0
53	50999.0
54	49987.0
55	49568.5
56	49150.0
57	47298.0
58	45446.0
59	41100.5
60	36755.0
61	32396.0
62	28037.0
63	24160.0
64	20283.0
65	17128.0
66	13973.0
67	11849.5
68	9726.0
69	8576.0
70	7426.0
71	5762.0
72	4098.0
73	3559.0
74	3020.0
75	2336.0
76	1652.0
77	1436.5
78	1221.0
79	1018.0
80	815.0
81	673.0
82	531.0
83	430.0
84	329.0
85	237.0
86	145.0
87	112.5
88	80.0
89	55.5
90	31.0
91	23.5
92	16.0
93	14.0
94	12.0
95	11.0
96	10.0
97	9.5
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0085309793424433
2	0.0011188169629433836
3	5.594084814716918E-4
4	0.0
5	0.0
6	2.797042407358459E-4
7	1.3985212036792295E-4
8	0.0
9	8.391127222075378E-4
10	6.992606018396149E-4
11	0.0
12	4.195563611037689E-4
13	5.594084814716918E-4
14	2.797042407358459E-4
15	0.0027970424073584595
16	5.594084814716918E-4
17	0.0
18	4.195563611037689E-4
19	0.0
20	5.594084814716918E-4
21	0.0
22	2.797042407358459E-4
23	0.0011188169629433836
24	2.797042407358459E-4
25	0.002237633925886767
26	0.004615119972141458
27	0.0065730496572923795
28	0.004475267851773534
29	0.0033564508888301514
30	0.004195563611037689
31	0.006852753898028226
32	0.004475267851773534
33	0.005034676333245227
34	0.003216598768462228
35	0.0036361551295659967
36	0.006712901777660303
37	0.0036361551295659967
38	0.008251275101707456
39	0.004754972092509381
40	0.003076746648094305
41	0.0033564508888301514
42	0.0018180775647829983
43	8.391127222075378E-4
44	0.0013985212036792297
45	0.0011188169629433836
46	8.391127222075378E-4
47	0.002657190286990536
48	0.0016782254444150757
49	4.195563611037689E-4
50	0.0016782254444150757
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	715041.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.67282939279347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.32865931838316	51.95081679449625
2	12.81519909368756	16.57590369241474
3	3.475940459413644	6.743967129335003
4	1.2983289780180833	3.3586643396433367
5	0.6384803006521235	2.0646163777367135
6	0.3489916443870045	1.3542166246170713
7	0.21569198345882812	0.9764587593338215
8	0.14096848156678707	0.7293464446503978
9	0.09913096754379294	0.5769972136352042
>10	0.5243831864469978	6.258850286173759
>50	0.06714053761380474	3.092377297057638
>100	0.045123171794294835	5.534934585086869
>500	0.0019618770339536852	0.7828504558192678
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	991	0.13859345128461167	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	757	0.10586805511851767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	2.797042407358459E-4
9	0.0	0.0	0.0	0.0	2.797042407358459E-4
10	0.0	0.0	0.0	0.0	2.797042407358459E-4
11	0.0	0.0	0.0	0.0	2.797042407358459E-4
12	0.0	0.0	0.0	0.0	2.797042407358459E-4
13	0.0	0.0	0.0	0.0	2.797042407358459E-4
14	0.0	0.0	0.0	0.0	4.1955636110376887E-4
15	0.0	0.0	0.0	0.0	4.1955636110376887E-4
16	0.0	0.0	0.0	0.0	4.1955636110376887E-4
17	0.0	0.0	0.0	0.0	4.1955636110376887E-4
18	0.0	0.0	0.0	0.0	5.594084814716918E-4
19	0.0	0.0	0.0	0.0	5.594084814716918E-4
20	0.0	0.0	0.0	0.0	6.992606018396148E-4
21	0.0	0.0	0.0	0.0	6.992606018396148E-4
22	0.0	0.0	0.0	5.594084814716918E-4	6.992606018396148E-4
23	0.0	0.0	0.0	0.0015383733240471526	8.391127222075377E-4
24	0.0	0.0	0.0	0.003076746648094305	8.391127222075377E-4
25	0.0	0.0	0.0	0.003216598768462228	8.391127222075377E-4
26	0.0	0.0	0.0	0.003636155129565997	8.391127222075377E-4
27	0.0	0.0	0.0	0.004335415731405612	8.391127222075377E-4
28	0.0	0.0	0.0	0.007272310259131994	8.391127222075377E-4
29	0.0	0.0	0.0	0.012167134472009298	8.391127222075377E-4
30	0.0	0.0	0.0	0.02167707865702806	8.391127222075377E-4
31	0.0	0.0	0.0	0.04237519247148066	8.391127222075377E-4
32	0.0	0.0	0.0	0.06559064445255587	8.391127222075377E-4
33	0.0	0.0	0.0	0.08586920190590469	8.391127222075377E-4
34	0.0	0.0	0.0	0.10908465388697991	8.391127222075377E-4
35	0.0	0.0	0.0	0.14041152884939465	8.391127222075377E-4
36	0.0	0.0	0.0	0.18054908739498854	8.391127222075377E-4
37	0.0	0.0	0.0	0.245020914884601	8.391127222075377E-4
38	0.0	0.0	0.0	0.32963144770719444	8.391127222075377E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCGT	50	8.342523E-5	26.397312	6
ATAGCGT	135	1.1277734E-10	21.183031	6
AGCGTAT	140	1.8553692E-10	20.426495	8
TAGCGTA	140	1.8553692E-10	20.426495	7
CGAGACT	130	1.6207196E-9	20.308466	20
CGTCGTA	120	1.4306352E-8	20.164614	10
TTAACGG	55	0.004479047	20.002163	35
TATGCCG	55	0.0044844896	19.997967	5
CGACGGT	170	5.456968E-12	19.40979	7
ACGACGG	170	5.456968E-12	19.40979	6
ACTTAGG	80	8.984223E-5	19.249388	1
CGTATAT	150	4.656613E-10	19.064726	10
CCGTCCA	105	1.792343E-6	18.855225	9
TACGACG	165	8.185452E-11	18.664768	5
CCGTCGT	130	3.58541E-8	18.613491	9
CTAATAC	190	1.8189894E-12	18.524431	3
AATAGCG	155	7.203198E-10	18.449738	5
TTGCGGT	60	0.007413564	18.331469	11
ACGAGAC	145	6.3027983E-9	18.205044	19
ACGAACG	145	6.3027983E-9	18.205044	15
>>END_MODULE