FastQCFastQC Report
Thu 2 Feb 2017
SRR4064185_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064185_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165154
Sequences flagged as poor quality0
Sequence length25
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT6050.3663247635540163No Hit
TATCAACGCAGAGTACTTTTTTTTT4690.2839773786889812No Hit
GAATAGGACCGCGGTTCTATTTTGT2490.15076837376024801No Hit
GCGCAAGACGGACCAGAGCGAAAGC2380.14410792351381135No Hit
GTATCTGATCGTCTTCGAACCTCCG2320.14047495065211862No Hit
ATCAGATACCGTCGTAGTTCCGACC2310.13986945517516983No Hit
CTATTGGAGCTGGAATTACCGCGGC2220.13441999588263076No Hit
GGTATCAACGCAGAGTACTTTTTTT2190.1326035094517844No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2140.12957603206704046No Hit
GATTAAGAGGGACGGCCGGGGGCAT2110.1277595456361941No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG2090.1265485546822965No Hit
GAATAATGGAATAGGACCGCGGTTC1900.11504414062026957No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1890.11443864514332078No Hit
TCGTAGTTCCGACCATAAACGATGC1850.11201666323552564No Hit
GAACTACGACGGTATCTGATCGTCT1800.10898918585078168No Hit
GAATAACGCCGCCGCATCGCCAGTC1750.10596170846603775No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1730.10475071751214018No Hit
GTCCTATTCCATTATTCCTAGCTGC1690.10232873560434502No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT1680.10172324012739625No Hit
CTTTAATATACGCTATTGGAGCTGG1680.10172324012739625No Hit
CATCTAAGGGCATCACAGACCTGTT1660.10051224917349867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGTG250.006006998519.0015147
ATCTAAG456.72853E-414.7744812
GTATTAG456.744011E-414.7700081
AGAACCG400.005249918414.2511375
CATTTGC400.005249918414.25113715
ATTTGCC400.005249918414.25113716
AGAATTT400.005249918414.25113717
CATCTAA500.001495591513.2930051
ACCTCCG604.0549747E-412.66767619
GTTCTAC604.075771E-412.6600061
AACCTCC701.078752E-412.2152618
CCGGTCC550.00304669712.0918739
TCTAAGG657.971904E-411.6896983
TGCCAAG600.005834577611.08421719
CGAACCT700.001478222810.85800816
TCTTCGA700.001478222810.85800812
TGCGCCG700.001478222810.8580085
GAACCTC700.001478222810.85800817
TTGCGCC803.740062E-410.6851154
GTATCAA2450.010.4638821
Produced by FastQC (version 0.11.5)