##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064185_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 165154 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.568263560071205 32.0 14.0 32.0 14.0 32.0 2 22.232262010002785 21.0 14.0 32.0 14.0 32.0 3 22.943791854874846 21.0 14.0 32.0 14.0 32.0 4 22.256845126366905 21.0 14.0 32.0 14.0 32.0 5 22.859500829528802 21.0 14.0 32.0 14.0 32.0 6 26.48191990505831 21.0 21.0 36.0 14.0 36.0 7 25.94822408176611 21.0 21.0 36.0 14.0 36.0 8 25.492249657895055 21.0 14.0 36.0 14.0 36.0 9 25.763227048693945 32.0 14.0 36.0 14.0 36.0 10 23.552078665972367 21.0 14.0 32.0 14.0 36.0 11 26.903962362401153 32.0 21.0 36.0 14.0 36.0 12 24.93259018855129 21.0 14.0 36.0 14.0 36.0 13 25.66716519127602 27.0 14.0 36.0 14.0 36.0 14 24.643193625343617 21.0 14.0 36.0 14.0 36.0 15 24.742785521392154 21.0 14.0 36.0 14.0 36.0 16 24.804733763638787 21.0 14.0 36.0 14.0 36.0 17 24.38671785121765 21.0 14.0 36.0 14.0 36.0 18 24.605973818375578 21.0 14.0 36.0 14.0 36.0 19 24.57909587415382 21.0 14.0 36.0 14.0 36.0 20 24.495670707339816 21.0 14.0 36.0 14.0 36.0 21 24.524322753309033 21.0 14.0 36.0 14.0 36.0 22 24.384780265691415 21.0 14.0 36.0 14.0 36.0 23 24.038097775409618 21.0 14.0 36.0 14.0 36.0 24 23.985546822965233 21.0 14.0 36.0 14.0 36.0 25 23.340706249924313 21.0 14.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores fail #Quality Count 3 1.0 4 2.0 5 8.0 6 7.0 7 13.0 8 10.0 9 2.0 10 8.0 11 9.0 12 7.0 13 3.0 14 551.0 15 1491.0 16 3282.0 17 6329.0 18 10200.0 19 13704.0 20 14992.0 21 14727.0 22 13257.0 23 11044.0 24 9501.0 25 8298.0 26 7292.0 27 6558.0 28 6158.0 29 5504.0 30 5082.0 31 5135.0 32 5115.0 33 5027.0 34 6199.0 35 5638.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.52836999709274 19.34356526795232 12.385526698323481 26.74253803663146 2 15.548477729067262 20.71354179281614 38.55329764817013 25.18468282994647 3 17.555236659340125 24.714053029614963 29.78874134893099 27.94196896211392 4 11.591815974471848 15.717737101198297 37.54685106357212 35.143595860757735 5 13.724991522550017 37.07309984013952 34.26827496003488 14.93363367727559 6 32.19166883655587 37.51082421475242 17.22124997729186 13.076256971399852 7 28.653529383266314 31.432585630541155 21.76372268785427 18.15016229833826 8 27.04274146170106 33.13568612250089 20.237540049300765 19.584032366497283 9 26.48672877269919 13.809708408543012 19.445891431548088 40.25767138720971 10 14.556743495774905 27.039403943423086 32.52869733773511 25.875155223066905 11 35.925688117852296 21.679346772630353 23.179152936615623 19.215812172901725 12 23.304923037781453 24.5134206843913 29.98285690055185 22.1987993772754 13 28.163655968693586 19.895443366166297 26.412362640691068 25.52853802444905 14 22.714991882526835 20.09074608059318 26.459327824759505 30.73493421212048 15 24.15299156171819 27.92326191384835 23.243135188180347 24.68061133625311 16 24.48947448129638 26.658791458427988 24.663334847796456 24.188399212479176 17 22.335166832246962 26.74222782233638 26.57018585378856 24.3524194916281 18 23.42467330259722 25.431810058099735 27.637661228273185 23.50585541102986 19 24.135633061315982 25.72169414102494 26.628620587290914 23.514052210368163 20 24.579973220074038 24.72599046343813 25.952898836103216 24.741137480384612 21 25.737739678308547 24.966528337321662 25.410595825886773 23.88513615848302 22 24.363557052672395 24.819154479031614 26.54824364768748 24.269044820608514 23 22.992565844082666 24.826566655962775 26.794467097649786 25.38640040230477 24 23.1387184131927 25.54510150792737 26.669534293382448 24.646645785497483 25 23.346440234348968 24.99197227557209 26.76170684567987 24.899880644399072 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 17.0 1 17.0 2 56.5 3 96.0 4 96.0 5 96.0 6 215.0 7 334.0 8 334.0 9 334.0 10 396.5 11 459.0 12 459.0 13 459.0 14 546.0 15 633.0 16 633.0 17 633.0 18 1113.5 19 1594.0 20 1594.0 21 1594.0 22 2507.5 23 3421.0 24 3421.0 25 3421.0 26 4890.0 27 6359.0 28 6359.0 29 6359.0 30 7675.0 31 8991.0 32 8991.0 33 8991.0 34 10755.0 35 12519.0 36 12519.0 37 12519.0 38 13794.0 39 15069.0 40 15069.0 41 15069.0 42 16574.5 43 18080.0 44 18080.0 45 18080.0 46 19776.0 47 21472.0 48 21472.0 49 21472.0 50 21409.0 51 21346.0 52 21346.0 53 21346.0 54 19200.5 55 17055.0 56 17055.0 57 17055.0 58 15316.5 59 13578.0 60 13578.0 61 13578.0 62 11912.5 63 10247.0 64 10247.0 65 10247.0 66 8397.5 67 6548.0 68 6548.0 69 6548.0 70 4938.5 71 3329.0 72 3329.0 73 3329.0 74 2576.5 75 1824.0 76 1824.0 77 1824.0 78 1571.5 79 1319.0 80 1319.0 81 1319.0 82 866.5 83 414.0 84 414.0 85 414.0 86 328.0 87 242.0 88 242.0 89 242.0 90 159.0 91 76.0 92 76.0 93 76.0 94 51.0 95 26.0 96 26.0 97 26.0 98 66.0 99 106.0 100 106.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03027477384743936 2 0.003632972861692723 3 6.054954769487872E-4 4 0.0018164864308463616 5 0.006054954769487872 6 0.009082432154231808 7 0.015742882400668467 8 0.02724729646269542 9 0.03632972861692723 10 0.04177918790946632 11 0.03996270147861995 12 0.044201169817261465 13 0.04601765624810783 14 0.0472286472020054 15 0.044201169817261465 16 0.04783414267895419 17 0.0472286472020054 18 0.056311079356237206 19 0.05510008840233963 20 0.06357702507962265 21 0.05388909744844206 22 0.05812756578708357 23 0.06357702507962265 24 0.056311079356237206 25 0.0611550431718275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 165154.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.26641077788042 #Duplication Level Percentage of deduplicated Percentage of total 1 87.31839284770572 57.862764889104035 2 7.892863143338066 10.460634225400655 3 1.8530425166344844 3.683834297885342 4 0.8120221424826608 2.1523917141796223 5 0.46828617332641354 1.5515821961624914 6 0.2436178208636819 0.9686207154098899 7 0.18470909600078725 0.8568006180999381 8 0.14876557726287537 0.7886528682008166 9 0.10583321691636863 0.6311868683512145 >10 0.8157144962376878 10.565432819830157 >50 0.10982692111744911 5.04883322892372 >100 0.04592762155820599 5.028984585643978 >500 9.984265556131737E-4 0.40028097280813146 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 605 0.3663247635540163 No Hit TATCAACGCAGAGTACTTTTTTTTT 469 0.2839773786889812 No Hit GAATAGGACCGCGGTTCTATTTTGT 249 0.15076837376024801 No Hit GCGCAAGACGGACCAGAGCGAAAGC 238 0.14410792351381135 No Hit GTATCTGATCGTCTTCGAACCTCCG 232 0.14047495065211862 No Hit ATCAGATACCGTCGTAGTTCCGACC 231 0.13986945517516983 No Hit CTATTGGAGCTGGAATTACCGCGGC 222 0.13441999588263076 No Hit GGTATCAACGCAGAGTACTTTTTTT 219 0.1326035094517844 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 214 0.12957603206704046 No Hit GATTAAGAGGGACGGCCGGGGGCAT 211 0.1277595456361941 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 209 0.1265485546822965 No Hit GAATAATGGAATAGGACCGCGGTTC 190 0.11504414062026957 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 189 0.11443864514332078 No Hit TCGTAGTTCCGACCATAAACGATGC 185 0.11201666323552564 No Hit GAACTACGACGGTATCTGATCGTCT 180 0.10898918585078168 No Hit GAATAACGCCGCCGCATCGCCAGTC 175 0.10596170846603775 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 173 0.10475071751214018 No Hit GTCCTATTCCATTATTCCTAGCTGC 169 0.10232873560434502 No Hit CTCCTGGTGGTGCCCTTCCGTCAAT 168 0.10172324012739625 No Hit CTTTAATATACGCTATTGGAGCTGG 168 0.10172324012739625 No Hit CATCTAAGGGCATCACAGACCTGTT 166 0.10051224917349867 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTGTG 25 0.0060069985 19.001514 7 ATCTAAG 45 6.72853E-4 14.774481 2 GTATTAG 45 6.744011E-4 14.770008 1 AGAACCG 40 0.0052499184 14.251137 5 CATTTGC 40 0.0052499184 14.251137 15 ATTTGCC 40 0.0052499184 14.251137 16 AGAATTT 40 0.0052499184 14.251137 17 CATCTAA 50 0.0014955915 13.293005 1 ACCTCCG 60 4.0549747E-4 12.667676 19 GTTCTAC 60 4.075771E-4 12.660006 1 AACCTCC 70 1.078752E-4 12.21526 18 CCGGTCC 55 0.003046697 12.091873 9 TCTAAGG 65 7.971904E-4 11.689698 3 TGCCAAG 60 0.0058345776 11.084217 19 CGAACCT 70 0.0014782228 10.858008 16 TCTTCGA 70 0.0014782228 10.858008 12 TGCGCCG 70 0.0014782228 10.858008 5 GAACCTC 70 0.0014782228 10.858008 17 TTGCGCC 80 3.740062E-4 10.685115 4 GTATCAA 245 0.0 10.463882 1 >>END_MODULE