FastQCFastQC Report
Thu 2 Feb 2017
SRR4064185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165154
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG4900.2966927837049057No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC2860.17317170640735313No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA1870.1132276541894232No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG1840.11141116775857685No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1820.11020017680467926No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG1780.10777819489688412No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1760.10656720394298655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA357.252407E-637.71428736
ATAATAC250.00234693435.23
GATGCGG505.893162E-835.234
ATGCGGC505.893162E-835.235
GGCGTTA551.3615681E-732.042
CGTTATT551.3615681E-732.044
TGCGGCG602.9194234E-729.33333236
AGACTGT407.0062815E-427.56
TCGCACG407.0062815E-427.513
GATCGCA407.0062815E-427.511
CCGATCG407.0062815E-427.59
CGATCGC407.0062815E-427.510
GCGTTAT655.87921E-727.07692343
GCGATGC655.87921E-727.07692332
CGATGCG655.87921E-727.07692333
TAGCGGC508.298695E-526.40000230
ATACGAA508.298695E-526.40000240
CAATACG508.298695E-526.40000238
CTAGCGG508.298695E-526.40000229
GGATGAA450.001393114724.4444451